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Blood, Vol. 95 No. 2 (January 15), 2000:
pp. 660-665
Elevated cytosolic phospholipase A2 expression and
activity in human neutrophils during sepsis
R. Levy,
R. Dana,
I. Hazan,
I. Levy,
G. Weber,
R. Smoliakov,
I. Pesach,
K. Riesenberg, and
F. Schlaeffer
From the Laboratory of Infectious Diseases and the Departments of
Clinical Biochemistry and Internal Medicine E, Faculty of Health
Sciences, Ben-Gurion University of the Neger and Soroka Medical Center,
Beer-Sheva, Israel.
Sepsis is defined as the systemic inflammatory response to
infection. Phospholipase A2 (PLA2) plays an
important role in inflammation processes by initiating the production
of inflammatory mediators. The role of cytosolic PLA
(cPLA2) has not yet been identified in inflammatory and
infectious disease clinical settings. The aim of the present research
was to determine whether cPLA2 activity has a role during
sepsis. Since neutrophil activation has been documented during sepsis,
these cells were chosen as a model to evaluate the function of
cPLA2 in this clinical setting. cPLA2 was studied at 3 levels: activity, protein expression, and messenger RNA
(mRNA). Neutrophils from 32 septic patients with and without bacteremia
were examined. cPLA2 activity was measured using labeled phosphatidyl choline vesicles as a substrate, and total
PLA2 was determined by the release of labeled arachidonic
acid from prelabeled cells. A significant increase in cPLA2
activity, protein expression, and total PLA2 activity in
neutrophils was detected during sepsis. mRNA levels, detected by
reverse transcriptase-polymerase chain reaction, were significantly
higher during sepsis, indicating that the increase in the amount of
cPLA2 is regulated on the mRNA level. The significant
elevation of cPLA2 activity and expression in neutrophils
during sepsis suggests that this enzyme plays a major role in
neutrophil function in this clinical setting.
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