|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 95 No. 3 (February 1), 2000:
pp. 952-958
Expression of VEGFR-2 and AC133 by circulating human
CD34+ cells identifies a population of functional
endothelial precursors
Mario Peichev,
Afzal J. Naiyer,
Daniel Pereira,
Zhenping Zhu,
William J. Lane,
Mathew Williams,
Mehmet C. Oz,
Daniel J. Hicklin,
Larry Witte,
Malcolm A. S. Moore, and
Shahin Rafii
From the Division of Hematology-Oncology, Weill Medical College of
Cornell University, New York, NY; Division of Molecular, Cell Biology
and Immunology, ImClone Systems, New York, NY; Department of
Cardiothoracic Surgery, Columbia-Presbyterian Medical Center, New York,
NY; and Division of Developmental Hematopoiesis, Sloan Kettering Cancer
Center, New York, NY.
Emerging data suggest that a subset of circulating human
CD34+ cells have phenotypic features of endothelial
cells. Whether these cells are sloughed mature endothelial cells or
functional circulating endothelial precursors (CEPs) is not known.
Using monoclonal antibodies (MoAbs) to the extracellular domain of the human vascular endothelial receptor-2 (VEGFR-2), we have shown that
1.2 ± 0.3% of CD34+ cells isolated from fetal liver
(FL), 2 ± 0.5% from mobilized peripheral blood, and 1.4 ± 0.5%
from cord blood were VEGFR-2+. In addition, most
CD34+VEGFR-2+ cells express hematopoietic
stem cell marker AC133. Because mature endothelial cells do not express
AC133, coexpression of VEGFR-2 and AC133 on CD34+ cells
phenotypically identifies a unique population of CEPs. CD34+VEGFR-2+ cells express
endothelial-specific markers, including VE-cadherin and E-selectin.
Also, virtually all CD34+VEGFR-2+ cells
express the chemokine receptor CXCR4 and migrate in response to stromal-derived factor (SDF)-1 or VEGF. To quantitate the plating efficiency of CD34+ cells that give rise to endothelial
colonies, CD34+ cells derived from FL were incubated with
VEGF and fibroblast growth factor (FGF)-2. Subsequent isolation and
plating of nonadherent FL-derived VEGFR-2+ cells with
VEGF and FGF-2 resulted in differentiation of AC133+
VEGFR-2+ cells into adherent
AC133 VEGFR-2+Ac-LDL+
(acetylated low-density lipoprotein) colonies (plating
efficiency of 3%). In an in vivo human model, we have found that the
neo-intima formed on the surface of left ventricular assist devices
is colonized with AC133+VEGFR-2+ cells.
These data suggest that circulating CD34+ cells
expressing VEGFR-2 and AC133 constitute a phenotypically and
functionally distinct population of circulating endothelial cells that
may play a role in neo-angiogenesis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. P.W. Rouhl, R. J. van Oostenbrugge, J. Damoiseaux, J.-W. C. Tervaert, and J. Lodder
Endothelial Progenitor Cell Research in Stroke: A Potential Shift in Pathophysiological and Therapeutical Concepts
Stroke,
July 1, 2008;
39(7):
2158 - 2165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Reddy, Z. Zhou, K. Schadler, S.-F. Jia, and E. S. Kleinerman
Bone Marrow Subsets Differentiate into Endothelial Cells and Pericytes Contributing to Ewing's Tumor Vessels
Mol. Cancer Res.,
June 1, 2008;
6(6):
929 - 936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Jelic, M. Padeletti, S. M. Kawut, C. Higgins, S. M. Canfield, D. Onat, P. C. Colombo, R. C. Basner, P. Factor, and T. H. LeJemtel
Inflammation, Oxidative Stress, and Repair Capacity of the Vascular Endothelium in Obstructive Sleep Apnea
Circulation,
April 29, 2008;
117(17):
2270 - 2278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. -T. Lee, K. Chu, K. -H. Jung, D. -H. Kim, E. -H. Kim, V. N. Choe, J. -H. Kim, W. -S. Im, L. Kang, J. -E. Park, et al.
Decreased number and function of endothelial progenitor cells in patients with migraine
Neurology,
April 22, 2008;
70(17):
1510 - 1517.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ria, C. Piccoli, T. Cirulli, F. Falzetti, G. Mangialardi, D. Guidolin, A. Tabilio, N. Di Renzo, A. Guarini, D. Ribatti, et al.
Endothelial Differentiation of Hematopoietic Stem and Progenitor Cells from Patients with Multiple Myeloma
Clin. Cancer Res.,
March 15, 2008;
14(6):
1678 - 1685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Hess, T. P. Craft, L. Wirthlin, S. Hohm, P. Zhou, W. C. Eades, M. H. Creer, M. S. Sands, and J. A. Nolta
Widespread Nonhematopoietic Tissue Distribution by Transplanted Human Progenitor Cells with High Aldehyde Dehydrogenase Activity
Stem Cells,
March 1, 2008;
26(3):
611 - 620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. P. Sieveking, A. Buckle, D. S. Celermajer, and M. K.C. Ng
Strikingly different angiogenic properties of endothelial progenitor cell subpopulations: insights from a novel human angiogenesis assay.
J. Am. Coll. Cardiol.,
February 12, 2008;
51(6):
660 - 668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Shantsila, T. Watson, H.-F. Tse, and G. Y.H. Lip
New insights on endothelial progenitor cell subpopulations and their angiogenic properties.
J. Am. Coll. Cardiol.,
February 12, 2008;
51(6):
669 - 671.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ieta, F. Tanaka, N. Haraguchi, Y. Kita, H. Sakashita, K. Mimori, T. Matsumoto, H. Inoue, H. Kuwano, and M. Mori
Biological and Genetic Characteristics of Tumor-Initiating Cells in Colon Cancer
Ann. Surg. Oncol.,
February 1, 2008;
15(2):
638 - 648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Atluri, C. M. Panlilio, G. P. Liao, E. E. Suarez, R. C. McCormick, W. Hiesinger, J. E. Cohen, M. J. Smith, A. B. Patel, W. Feng, et al.
Transmyocardial revascularization to enhance myocardial vasculogenesis and hemodynamic function.
J. Thorac. Cardiovasc. Surg.,
February 1, 2008;
135(2):
283 - 291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Whittaker, J. S. Moore, M. Vasa-Nicotera, S. Stevens, and N. J. Samani
Evidence for genetic regulation of endothelial progenitor cells and their role as biological markers of atherosclerotic susceptibility
Eur. Heart J.,
February 1, 2008;
29(3):
332 - 338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Balestrieri, C. Schiano, F. Felice, A. Casamassimi, A. Balestrieri, L. Milone, L. Servillo, and C. Napoli
Effect of Low Doses of Red Wine and Pure Resveratrol on Circulating Endothelial Progenitor Cells
J. Biochem.,
February 1, 2008;
143(2):
179 - 186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Tang, J. Lu, J. P. Walterscheid, H.-H. Chen, D. A. Engler, T. Sawamura, P.-Y. Chang, H. J. Safi, C.-Y. Yang, and C.-H. Chen
Electronegative LDL circulating in smokers impairs endothelial progenitor cell differentiation by inhibiting Akt phosphorylation via LOX-1
J. Lipid Res.,
January 1, 2008;
49(1):
33 - 47.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mints, M. Jansson, B. Sadeghi, M. Westgren, M. Uzunel, M. Hassan, and J. Palmblad
Endometrial endothelial cells are derived from donor stem cells in a bone marrow transplant recipient
Hum. Reprod.,
January 1, 2008;
23(1):
139 - 143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W Wojakowski, M Kucia, M Kazmierski, M Z Ratajczak, and M Tendera
Circulating progenitor cells in stable coronary heart disease and acute coronary syndromes: relevant reparatory mechanism?
Heart,
January 1, 2008;
94(1):
27 - 33.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Kleinman, M. R. Greives, S. S. Churgin, K. M. Blechman, E. I. Chang, D. J. Ceradini, O. M. Tepper, and G. C. Gurtner
Hypoxia-Induced Mediators of Stem/Progenitor Cell Trafficking Are Increased in Children With Hemangioma
Arterioscler. Thromb. Vasc. Biol.,
December 1, 2007;
27(12):
2664 - 2670.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Diez, J. A. Barbera, E. Ferrer, R. Fernandez-Lloris, S. Pizarro, J. Roca, and V. I. Peinado
Plasticity of CD133+ cells: Role in pulmonary vascular remodeling
Cardiovasc Res,
December 1, 2007;
76(3):
517 - 527.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kanayasu-Toyoda, A. Ishii-Watabe, T. Suzuki, T. Oshizawa, and T. Yamaguchi
A New Role of Thrombopoietin Enhancing ex Vivo Expansion of Endothelial Precursor Cells Derived from AC133-positive Cells
J. Biol. Chem.,
November 16, 2007;
282(46):
33507 - 33514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Santini, B. Vincenzi, and G. Tonini
Zoledronic Acid and Angiogenesis
Clin. Cancer Res.,
November 15, 2007;
13(22):
6850 - 6851.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Farha, K. Asosingh, D. Laskowski, L. Licina, H. Sekigushi, D. W. Losordo, R. A. Dweik, H. P. Wiedemann, and S. C. Erzurum
Pulmonary gas transfer related to markers of angiogenesis during the menstrual cycle
J Appl Physiol,
November 1, 2007;
103(5):
1789 - 1795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Zhou, K. Reddy, H. Guan, and E. S. Kleinerman
VEGF165, but not VEGF189, Stimulates Vasculogenesis and Bone Marrow Cell Migration into Ewing's Sarcoma Tumors In vivo
Mol. Cancer Res.,
November 1, 2007;
5(11):
1125 - 1132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wu, M. W. Lensch, J. Wylie-Sears, G. Q. Daley, and J. Bischoff
Hemogenic Endothelial Progenitor Cells Isolated from Human Umbilical Cord Blood
Stem Cells,
November 1, 2007;
25(11):
2770 - 2776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shmilovich, V. Deutsch, A. Roth, H. Miller, G. Keren, and J. George
Circulating endothelial progenitor cells in patients with cardiac syndrome X
Heart,
September 1, 2007;
93(9):
1071 - 1076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Wang, Y. Zheng, W. Zhang, H. Yu, K. Lou, Y. Zhang, Q. Qin, B. Zhao, Y. Yang, and R. Hui
Polymorphisms of KDR Gene Are Associated With Coronary Heart Disease
J. Am. Coll. Cardiol.,
August 21, 2007;
50(8):
760 - 767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ferrario, M. Massa, V. Rosti, R. Campanelli, M. Ferlini, B. Marinoni, G. M. De Ferrari, V. Meli, M. De Amici, A. Repetto, et al.
Early haemoglobin-independent increase of plasma erythropoietin levels in patients with acute myocardial infarction
Eur. Heart J.,
August 1, 2007;
28(15):
1805 - 1813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wojakowski, M. Kazmierski, B. Korzeniowska, and M. Tendera
Link between erythropoietin release and mobilization of endothelial progenitor cells in acute myocardial infarction
Eur. Heart J.,
August 1, 2007;
28(15):
1785 - 1786.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Liu, D. D. Swartz, H. F. Peng, S. F. Gugino, J. A. Russell, and S. T. Andreadis
Functional tissue-engineered blood vessels from bone marrow progenitor cells
Cardiovasc Res,
August 1, 2007;
75(3):
618 - 628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Schmidt, K. Brixius, and W. Bloch
Endothelial Precursor Cell Migration During Vasculogenesis
Circ. Res.,
July 20, 2007;
101(2):
125 - 136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Timmermans, F. Van Hauwermeiren, M. De Smedt, R. Raedt, F. Plasschaert, M. L. De Buyzere, T. C. Gillebert, J. Plum, and B. Vandekerckhove
Endothelial Outgrowth Cells Are Not Derived From CD133+ Cells or CD45+ Hematopoietic Precursors
Arterioscler. Thromb. Vasc. Biol.,
July 1, 2007;
27(7):
1572 - 1579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Yu, X. Sun, Y. Qiu, J. Zhou, Y. Wu, L. Zhuang, J. Chen, and Y. Ding
Identification and Clinical Significance of Mobilized Endothelial Progenitor Cells in Tumor Vasculogenesis of Hepatocellular Carcinoma
Clin. Cancer Res.,
July 1, 2007;
13(13):
3814 - 3824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Rohde, C. Bartmann, K. Schallmoser, A. Reinisch, G. Lanzer, W. Linkesch, C. Guelly, and D. Strunk
Immune Cells Mimic the Morphology of Endothelial Progenitor Colonies In Vitro
Stem Cells,
July 1, 2007;
25(7):
1746 - 1752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nagano, T. Yamashita, H. Hamada, K. Ohneda, K.-i. Kimura, T. Nakagawa, M. Shibuya, H. Yoshikawa, and O. Ohneda
Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood
Blood,
July 1, 2007;
110(1):
151 - 160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. K. Kissel, R. Lehmann, B. Assmus, A. Aicher, J. Honold, U. Fischer-Rasokat, C. Heeschen, I. Spyridopoulos, S. Dimmeler, and A. M. Zeiher
Selective Functional Exhaustion of Hematopoietic Progenitor Cells in the Bone Marrow of Patients With Postinfarction Heart Failure
J. Am. Coll. Cardiol.,
June 19, 2007;
49(24):
2341 - 2349.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. V. Pfenninger, T. Roschupkina, F. Hertwig, D. Kottwitz, E. Englund, J. Bengzon, S. E. Jacobsen, and U. A. Nuber
CD133 Is Not Present on Neurogenic Astrocytes in the Adult Subventricular Zone, but on Embryonic Neural Stem Cells, Ependymal Cells, and Glioblastoma Cells
Cancer Res.,
June 15, 2007;
67(12):
5727 - 5736.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Nolan, A. Ciarrocchi, A. S. Mellick, J. S. Jaggi, K. Bambino, S. Gupta, E. Heikamp, M. R. McDevitt, D. A. Scheinberg, R. Benezra, et al.
Bone marrow-derived endothelial progenitor cells are a major determinant of nascent tumor neovascularization
Genes & Dev.,
June 15, 2007;
21(12):
1546 - 1558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Bezuidenhout, M. Bracher, G. Davison, P. Zilla, and N. Davies
Ang-2 and PDGF-BB cooperatively stimulate human peripheral blood monocyte fibrinolysis
J. Leukoc. Biol.,
June 1, 2007;
81(6):
1496 - 1503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Invernici, C. Emanueli, P. Madeddu, S. Cristini, S. Gadau, A. Benetti, E. Ciusani, G. Stassi, M. Siragusa, R. Nicosia, et al.
Human Fetal Aorta Contains Vascular Progenitor Cells Capable of Inducing Vasculogenesis, Angiogenesis, and Myogenesis in Vitro and in a Murine Model of Peripheral Ischemia
Am. J. Pathol.,
June 1, 2007;
170(6):
1879 - 1892.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Melero-Martin, Z. A. Khan, A. Picard, X. Wu, S. Paruchuri, and J. Bischoff
In vivo vasculogenic potential of human blood-derived endothelial progenitor cells
Blood,
June 1, 2007;
109(11):
4761 - 4768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Dentelli, A. Rosso, A. Balsamo, S. Colmenares Benedetto, A. Zeoli, M. Pegoraro, G. Camussi, L. Pegoraro, and M. F. Brizzi
C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium
Blood,
May 15, 2007;
109(10):
4264 - 4271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. R. Santhanam, L. A. Smith, T. He, K. A. Nath, and Z. S. Katusic
Endothelial Progenitor Cells Stimulate Cerebrovascular Production of Prostacyclin By Paracrine Activation of Cyclooxygenase-2
Circ. Res.,
May 11, 2007;
100(9):
1379 - 1388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. D. Theiss, R. David, M. G. Engelmann, A. Barth, K. Schotten, M. Naebauer, B. Reichart, G. Steinbeck, and W.-M. Franz
Circulation of CD34+ progenitor cell populations in patients with idiopathic dilated and ischaemic cardiomyopathy (DCM and ICM)
Eur. Heart J.,
May 2, 2007;
28(10):
1258 - 1264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
