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Blood, Vol. 95 No. 5 (March 1), 2000:
pp. 1652-1655
The GATA-E box-GATA motif in the EKLF promoter is required for
in vivo expression
Kathleen P. Anderson,
Scott C. Crable, and
Jerry
B. Lingrel
From the Department of Molecular Genetics, Biochemistry, and
Microbiology, University of Cincinnati, Cincinnati, OH.
The erythroid Krüppel-like factor (EKLF) is a key regulatory
protein in globin gene expression. This zinc finger transcription factor is required for expression of the adult  globin gene, and it
has been suggested that it plays an important role in the developmental
switch from fetal  to adult globin gene expression. We have
previously described a sequence element in the distal promoter region
of the mouse EKLF gene that is critical for the expression of this
transcription factor. The element consists of an E box motif flanked by
2 GATA-1 binding sites. Here we demonstrate that mutation of the E box
or the GATA-1 consensus sequences eliminates expression from the EKLF
promoter in transgenic mice. These results confirm the importance of
this activator element for in vivo expression of the EKLF gene.
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