|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 95 No. 9 (May 1), 2000:
pp. 2990-2992
BRIEF REPORT
Quantitative measure of c-abl and
p15 methylation in chronic myelogenous leukemia: biological
implications
TuDung T. Nguyen,
Ann F. Mohrbacher,
Yvonne C. Tsai,
John Groffen,
Nora Heisterkamp,
Peter
W. Nichols,
Mimi C. Yu,
Michael Lübbert, and
Peter A. Jones
From the Departments of Biochemistry & Molecular
Biology; Hematology/Oncology; Pathology; and Preventive Medicine,
USC/Norris Cancer Center, University of Southern California, Los
Angeles, CA; the Division of Hematology/Oncology,
Children's Hospital, Los Angeles, CA; and the Division of
Hematology/Oncology, the Medical University of Freiburg Medical Center,
Freiburg, Germany.
We used a sensitive, quantitative bisulfite PCR assay, methylation
sensitive single nucleotide primer extension (Ms-SNuPE), to
measure methylation of the 5' CpG islands of c-abl and
p15 in chronic myelogenous leukemia (CML) patients during
progression. We found that the Pa promoter of c-abl was
methylated in 81% (17/21) of the white blood cells (WBCs) of CML
patients, which correlates with previous reports. In contrast, WBCs
from healthy donors, acute myelogenous leukemias, acute lymphocytic
leukemias, and myelodysplastic syndromes were unmethylated at the
c-abl Pa promoter locus. We also observed p15
hypermethylation in 24% (8/34) of CML cases. Methylation of the
p15 but not c-abl Pa promoters was associated with CML
progression (P = 0.047 vs 0.46), and the two events were
independently acquired. We conclude that de novo methylation of
c-abl and p15 both occur in CML, and analysis of DNA
methylation changes using the bisulfite-based MS-SNuPE assay allows
both a sensitive and quantitative assessment of these molecular events compared to other methods currently utilized.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Cortes and H. Kantarjian
New Targeted Approaches in Chronic Myeloid Leukemia
J. Clin. Oncol.,
September 10, 2005;
23(26):
6316 - 6324.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. A. Kaminsky, A. Assadzadeh, J. Flanagan, and A. Petronis
Single nucleotide extension technology for quantitative site-specific evaluation of metC/C in GC-rich regions
Nucleic Acids Res.,
June 15, 2005;
33(10):
e95 - e95.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kusy, M. Cividin, N. Sorel, F. Brizard, F. Guilhot, A. Brizard, C. Larsen, and J. Roche
p14ARF, p15INK4b, and p16INK4a methylation status in chronic myelogenous leukemia
Blood,
January 1, 2003;
101(1):
374 - 374.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Daskalakis, T. T. Nguyen, C. Nguyen, P. Guldberg, G. Kohler, P. Wijermans, P. A. Jones, and M. Lubbert
Demethylation of a hypermethylated P15/INK4B gene in patients with myelodysplastic syndrome by 5-Aza-2'-deoxycytidine (decitabine) treatment
Blood,
September 26, 2002;
100(8):
2957 - 2964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Nguyen, G. Liang, T. T. Nguyen, D. Tsao-Wei, S. Groshen, M. Lubbert, J.-H. Zhou, W. F. Benedict, and P. A. Jones
Susceptibility of Nonpromoter CpG Islands to De Novo Methylation in Normal and Neoplastic Cells
J Natl Cancer Inst,
October 3, 2001;
93(19):
1465 - 1472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Sun, G. Jiang, M.-A. A. Zaydan, V. F. La Russa, H. Safah, and M. Ehrlich
ABL1 Promoter Methylation Can Exist Independently of BCR-ABL Transcription in Chronic Myeloid Leukemia Hematopoietic Progenitors
Cancer Res.,
September 1, 2001;
61(18):
6931 - 6937.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. D.C. Markl, J. Cheng, G. Liang, D. Shibata, P. W. Laird, and P. A. Jones
Global and Gene-specific Epigenetic Patterns in Human Bladder Cancer Genomes Are Relatively Stable in Vivo and in Vitro over Time
Cancer Res.,
August 1, 2001;
61(15):
5875 - 5884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F Costello and C. Plass
Methylation matters
J. Med. Genet.,
May 1, 2001;
38(5):
285 - 303.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
O. Galm, M. R. Rountree, K. E. Bachman, K.-W. Jair, S. B. Baylin, and J. G. Herman
Enzymatic Regional Methylation Assay: A Novel Method to Quantify Regional CpG Methylation Density
Genome Res.,
January 1, 2002;
12(1):
153 - 157.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
