|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 December 2000, Vol. 96, No. 12, pp. 3958-3963
PHAGOCYTES
Expression of CCR6 and CD83 by cytokine-activated
human neutrophils
Shigeo Yamashiro,
Ji-Ming Wang,
De Yang,
Wang-Hua Gong,
Hidenobu Kamohara, and
Teizo Yoshimura
From the Laboratory of Molecular Immunoregulation,
National Cancer Institute-Frederick Cancer Research and Development
Center, Frederick, MD.
Polymorphonuclear leukocytes (PMNLs) are thought to be terminally
differentiated, short-lived, and unable to actively synthesize new
proteins or to interact with T cells. In the current study, it was
found that PMNLs incubated with supernatants of phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PHA-sup) expressed
high levels of CCR6 mRNA. Neutralization with IgG against several
cytokines revealed that tumor necrosis factor (TNF)- was largely
responsible for the PHA-sup-induced CCR6 mRNA expression. Among
recombinant cytokines, TNF- induced high levels of CCR6 mRNA
expression, whereas interferon (IFN)- induced low levels. The 2 cytokines together exhibited a considerable synergy. Cytokine-activated PMNLs expressed functional CCR6, as detected by the binding of sodium
iodide I 125-labeled liver and activation-regulated chemokine (LARC)
and dose-dependent migration toward LARC. The induction of CCR6
suggested that these cytokine-activated PMNLs have more similarities
with dendritic cells (DCs) that express CCR6 in an immature stage. In
fact, the activation of PMNLs with TNF- and IFN- induced the
expression of CD83, a dominant cell-surface marker of DCs. When PMNLs
were activated with granulocyte macrophage-colony-stimulating factor,
TNF-, and IFN-, these cells expressed CD40 and HLA-DR in addition
to CD83. Taken together, PMNLs, under appropriate conditions, can
undergo a differentiation process characterized by the acquisition of
new phenotypes and functions, and such differentiated PMNLs may play
more active roles in the adaptive immune response.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. P. Singh, H. H. Zhang, J. F. Foley, M. N. Hedrick, and J. M. Farber
Human T Cells That Are Able to Produce IL-17 Express the Chemokine Receptor CCR6
J. Immunol.,
January 1, 2008;
180(1):
214 - 221.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yoshimura and M. Takahashi
IFN-{gamma}-Mediated Survival Enables Human Neutrophils to Produce MCP-1/CCL2 in Response to Activation by TLR Ligands
J. Immunol.,
August 1, 2007;
179(3):
1942 - 1949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. K. Demedts, K. R. Bracke, G. Van Pottelberge, D. Testelmans, G. M. Verleden, F. E. Vermassen, G. F. Joos, and G. G. Brusselle
Accumulation of Dendritic Cells and Increased CCL20 Levels in the Airways of Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med.,
May 15, 2007;
175(10):
998 - 1005.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Katchar, C. P. Kelly, S. Keates, M. J. O'Brien, and A. C. Keates
MIP-3{alpha} neutralizing monoclonal antibody protects against TNBS-induced colonic injury and inflammation in mice
Am J Physiol Gastrointest Liver Physiol,
May 1, 2007;
292(5):
G1263 - G1271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nishimura, T. Myles, A. M. Piliposky, P. N. Kao, G. J. Berry, and L. L. K. Leung
Thrombin-activatable procarboxypeptidase B regulates activated complement C5a in vivo
Blood,
March 1, 2007;
109(5):
1992 - 1997.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. Bracke, A. I. D'hulst, T. Maes, K. B. Moerloose, I. K. Demedts, S. Lebecque, G. F. Joos, and G. G. Brusselle
Cigarette Smoke-Induced Pulmonary Inflammation and Emphysema Are Attenuated in CCR6-Deficient Mice
J. Immunol.,
October 1, 2006;
177(7):
4350 - 4359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E-J D Oudijk, E H J Nijhuis, M D Zwank, E A van de Graaf, H J Mager, P J Coffer, J-W J Lammers, and L Koenderman
Systemic inflammation in COPD visualised by gene profiling in peripheral blood neutrophils
Thorax,
July 1, 2005;
60(7):
538 - 544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kurosaka, Q. Chen, F. Yarovinsky, J. J. Oppenheim, and D. Yang
Mouse Cathelin-Related Antimicrobial Peptide Chemoattracts Leukocytes Using Formyl Peptide Receptor-Like 1/Mouse Formyl Peptide Receptor-Like 2 as the Receptor and Acts as an Immune Adjuvant
J. Immunol.,
May 15, 2005;
174(10):
6257 - 6265.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Klein, S. Koch, B. Borm, J. Neumann, V. Herzog, N. Koch, and T. Bieber
CD83 localization in a recycling compartment of immature human monocyte-derived dendritic cells
Int. Immunol.,
April 1, 2005;
17(4):
477 - 487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Munster, K. P. A. MacDonald, M. Kato, and D. J. N. Hart
Human T lymphoblasts and activated dendritic cells in the allogeneic mixed leukocyte reaction are susceptible to NK cell-mediated anti-CD83-dependent cytotoxicity
Int. Immunol.,
January 1, 2004;
16(1):
33 - 42.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Yang, H. F. Rosenberg, Q. Chen, K. D. Dyer, K. Kurosaka, and J. J. Oppenheim
Eosinophil-derived neurotoxin (EDN), an antimicrobial protein with chemotactic activities for dendritic cells
Blood,
November 1, 2003;
102(9):
3396 - 3403.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Dudziak, A. Kieser, U. Dirmeier, F. Nimmerjahn, S. Berchtold, A. Steinkasserer, G. Marschall, W. Hammerschmidt, G. Laux, and G. W. Bornkamm
Latent Membrane Protein 1 of Epstein-Barr Virus Induces CD83 by the NF-{kappa}B Signaling Pathway
J. Virol.,
August 1, 2003;
77(15):
8290 - 8298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J H Kwon, S Keates, L Bassani, L F Mayer, and A C Keates
Colonic epithelial cells are a major site of macrophage inflammatory protein 3{alpha} (MIP-3{alpha}) production in normal colon and inflammatory bowel disease
Gut,
December 1, 2002;
51(6):
818 - 826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Niyonsaba, K. Iwabuchi, H. Matsuda, H. Ogawa, and I. Nagaoka
Epithelial cell-derived human {beta}-defensin-2 acts as a chemotaxin for mast cells through a pertussis toxin-sensitive and phospholipase C-dependent pathway
Int. Immunol.,
April 1, 2002;
14(4):
421 - 426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Sabroe, C.M. Lloyd, M.K.B. Whyte, S.K. Dower, T.J. Williams, and J.E. Pease
Chemokines, innate and adaptive immunity, and respiratory disease
Eur. Respir. J.,
February 1, 2002;
19(2):
350 - 355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Yamashiro, H. Kamohara, J.-M. Wang, D. Yang, W.-H. Gong, and T. Yoshimura
Phenotypic and functional change of cytokine-activated neutrophils: inflammatory neutrophils are heterogeneous and enhance adaptive immune responses
J. Leukoc. Biol.,
May 1, 2001;
69(5):
698 - 704.
[Abstract]
[Full Text]
|
|
|
| |
