|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 December 2000, Vol. 96, No. 12, pp. 3971-3978
TRANSPLANTATION
Donor stromal cells from human blood engraft in NOD/SCID
mice
Silvia-Renate Goan,
Ilse Junghahn,
Manuela Wissler,
Michael Becker,
Jutta Aumann,
Ursula Just,
Georg Martiny-Baron,
Iduna Fichtner, and
Reinhard Henschler
From the Max Delbrück Center for Molecular
Medicine, Berlin, Germany; Department of Hematology and Oncology,
University Medical Center, Freiburg, Germany; Paterson Institute for
Cancer Research, Manchester, England; Institute of Molecular Medicine,
Tumor Biology Center, Freiburg, Germany; and Institute of
Transfusion Medicine and Immune Hematology, Frankfurt, Germany.
Little is known about the presence, frequency, and in vivo
proliferative potential of stromal cells within blood-derived
hematopoietic transplants. In this study, nonobese diabetic/severe
combined immunodeficiency (NOD/SCID) mice were injected with human
CD34+ peripheral blood cells (PBCs) or cord blood cells
(CBCs, either enriched for CD34 or density-gradient separated
mononuclear cells). Flow cytometric analysis 5 to 11 weeks after
transplantation revealed the presence of a human lymphomyeloid
hematopoiesis within the murine bone marrow. Immunohistochemical
staining of bone marrow cell suspensions using human-specific
antibodies showed human cells staining positive for human fibroblast
markers, human von Willebrand factor (vWF) and human KDR
(vascular endothelial growth factor receptor-2) in mice transplanted
with CD34+ PBCs or CBCs, with mean frequencies between
0.6% and 2.4%. In stromal layers of bone marrow cultures established
from the mice, immunohistochemical staining using human-specific
antibodies revealed flattened reticular cells or spindle-shaped cells
staining positive with human-specific antifibroblast antibodies (mean
frequency, 2.2%). Cell populations of more rounded cells stained
positive with human-specific antibodies recognizing CD34 (1.5%), vWF
(2.2%), and KDR (1.6%). Reverse transcriptase-polymerase chain
reaction (RT-PCR) analysis and subsequent complementary DNA sequencing detected transcripts of human KDR (endothelial specific) and human proline hydroxylase- (fibroblast specific) within the bone marrow and spleen of transplanted mice. Analysis of nontransplanted control mice yielded negative results in immunocytochemistry and RT-PCR. Cells
expressing endothelial and fibroblast markers were also detected in the
grafts before transplantation, and their numbers increased up to 3 log
in vivo after transplantation. These results indicate that stromal
progenitor cells are present in human cytokine-mobilized peripheral
blood or cord blood that engraft in NOD/SCID mice.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. Ruster, S. Gottig, R. J. Ludwig, R. Bistrian, S. Muller, E. Seifried, J. Gille, and R. Henschler
Mesenchymal stem cells display coordinated rolling and adhesion behavior on endothelial cells
Blood,
December 1, 2006;
108(12):
3938 - 3944.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ogawa, A. C. LaRue, and C. J. Drake
Hematopoietic origin of fibroblasts/myofibroblasts: its pathophysiologic implications
Blood,
November 1, 2006;
108(9):
2893 - 2896.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Epperly, H. Guo, J. E. Gretton, and J. S. Greenberger
Bone Marrow Origin of Myofibroblasts in Irradiation Pulmonary Fibrosis
Am. J. Respir. Cell Mol. Biol.,
August 1, 2003;
29(2):
213 - 224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Harder, R. Henschler, I. Junghahn, M. C. Lamers, and A. M. Muller
Human hematopoiesis in murine embryos after injecting human cord blood-derived hematopoietic stem cells into murine blastocysts
Blood,
January 15, 2002;
99(2):
719 - 721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
