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Blood, 1 December 2000, Vol. 96, No. 12, pp. 3991-3994
BRIEF REPORT
Polyclonal hematopoiesis with variable telomere
shortening in human long-term allogeneic marrow graft
recipients
George Mathioudakis,
Rainer Storb,
Peter A. McSweeney,
Beverly Torok-Storb,
Peter M. Lansdorp,
Tim H. Brümmendorf,
M. John Gass,
Eileen M. Bryant,
Jan Storek,
Mary E. D. Flowers,
Ted Gooley, and
Richard A. Nash
From the Fred Hutchinson Cancer Research Center and the
University of Washington School of Medicine, Seattle, WA; and The Terry
Fox Laboratory, Vancouver, BC, Canada.
Donor-derived hematopoiesis was assessed in 17 patients who
received allogeneic marrow grafts from HLA-matched siblings between 1971 and 1980. Complete blood counts were normal or near normal in all
patients except one. Chimerism analyses, using either dual-color XY-chromosome fluorescence in situ hybridization (FISH) or analysis of
variable number tandem repeat loci, indicated that 15 out of 16 patients had greater than 97% donor-derived hematopoiesis, whereas 1 patient had indeterminate chimerism. All 12 recipients of grafts from
female donors exhibited polyclonal hematopoiesis by X-linked
clonal analysis with the use of molecular probes. Of the 17 recipients,
9 exhibited a less than 1.0-kilobase shortening of granulocyte telomere
length compared with their respective donors, according to
terminal restriction fragment analysis or flow-FISH with a
fluorescein-labeled peptide nucleic acid probe. These data suggest that
under standard transplantation conditions, the stem cell proliferative
potential is not compromised during hematopoietic reconstitution.
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