|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 2 (July 15), 2000:
pp. 506-513
VLA-4 ( 4 1) engagement defines a
novel activation pathway for 2 integrin-dependent
leukocyte adhesion involving the urokinase receptor
Andreas E. May,
Franz-Josef Neumann,
Albert Schömig, and
Klaus T. Preissner
From the Deutsches Herzzentrum und 1. Medizinische Klinik des
Klinikums Rechts der Isar, Technische Universität, D-80636
München, Germany; and Institut für Biochemie, Fachbereich
Humanmedizin, Justus-Liebig-Universität, D-35392 Giessen,
Germany.
During acute inflammatory processes,  2 and
1 integrins sequentially mediate leukocyte recruitment
into extravascular tissues. We studied the influence of VLA-4 (very
late antigen-4) ( 41) engagement on 2 integrin activation-dependent
cell-to-cell adhesion. Ligation of VLA-4 by the soluble chimera fusion
product vascular cell adhesion molecule-1 (VCAM-1)-Fc or by 2 anti-CD29 (1 chain) monoclonal antibodies (mAb) rapidly
induced adhesion of myelomonocytic cells (HL60, U937) to human
umbilical vein endothelial cells (HUVECs). Cell adhesion was mediated
via 2 integrin (LFA-1 and Mac-1) activation: induced
adhesion to HUVECs was inhibited by blocking mAbs anti-CD18 (70%-90%), anti-CD11a (50%-60%), or anti-CD11b (60%-70%).
Adhesion to immobilized ligands of 2 integrins
(intercellular adhesion molecule-1 [ICAM-1], fibrinogen, keyhole
limpet hemocyanin) as well as to ICAM-1-transfected Chinese hamster
ovary cells, but not to ligands of 1 integrins (VCAM-1,
fibronectin, laminin, and collagen), was augmented. VCAM-1-Fc binding
provoked the expression of the activation-dependent epitope CBRM1/5 of
Mac-1 on leukocytes. Clustering of VLA-4 through dimeric VCAM-1-Fc was
required for 2 integrin activation and induction of cell
adhesion, whereas monovalent VCAM-1 or Fab fragments of
anti-1 integrin mAb were ineffective. Activation of
2 integrins by 41 integrin
ligation (VCAM-1-Fc or anti-1 mAb) required the
presence of urokinase receptor (uPAR) on leukocytic cells, because the
removal of uPAR from the cell surface by phosphatidylinositol-specific
phospholipase C reduced cell adhesion to less than 40%. Adhesion was
reconstituted when soluble recombinant uPAR was allowed to reassociate
with the cells. Finally, VLA-4 engagement by VCAM-1-Fc or
anti-1 integrin mAb induced uPAR-dependent adhesion to
immobilized vitronectin as well. These results elucidate a novel
activation pathway of 2 integrin-dependent cell-to-cell
adhesion that requires 41 integrin
ligation for initiation and uPAR as activation transducer.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Viswanathan, J. Richardson, B. Togonu-Bickersteth, E. Dai, L. Liu, P. Vatsya, Y.-m. Sun, J. Yu, G. Munuswamy-Ramanujam, H. Baker, et al.
Myxoma viral serpin, Serp-1, inhibits human monocyte adhesion through regulation of actin-binding protein filamin B
J. Leukoc. Biol.,
March 1, 2009;
85(3):
418 - 426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. May, P. Seizer, and M. Gawaz
Platelets: Inflammatory Firebugs of Vascular Walls
Arterioscler Thromb Vasc Biol,
March 1, 2008;
28(3):
s5 - s10.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Yoshimi, S. Yamaji, A. Suzuki, W. Mishima, M. Okamura, T. Obana, C. Matsuda, Y. Miwa, S. Ohno, and Y. Ishigatsubo
The {gamma}-Parvin-Integrin-Linked Kinase Complex Is Critically Involved in Leukocyte-Substrate Interaction
J. Immunol.,
March 15, 2006;
176(6):
3611 - 3624.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Schmidt, A. Bultmann, M. Ungerer, N. Joghetaei, O. Bulbul, S. Thieme, T. Chavakis, B. P. Toole, M. Gawaz, A. Schomig, et al.
Extracellular Matrix Metalloproteinase Inducer Regulates Matrix Metalloproteinase Activity in Cardiovascular Cells: Implications in Acute Myocardial Infarction
Circulation,
February 14, 2006;
113(6):
834 - 841.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J. Till, D. G. Spiller, R. J. Harris, H. Chen, M. Zuzel, and J. C. Cawley
CLL, but not normal, B cells are dependent on autocrine VEGF and {alpha}4{beta}1 integrin for chemokine-induced motility on and through endothelium
Blood,
June 15, 2005;
105(12):
4813 - 4819.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Selleri, N. Montuori, P. Ricci, V. Visconte, M. V. Carriero, N. Sidenius, B. Serio, F. Blasi, B. Rotoli, G. Rossi, et al.
Involvement of the urokinase-type plasminogen activator receptor in hematopoietic stem cell mobilization
Blood,
March 1, 2005;
105(5):
2198 - 2205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Hyduk, J. Oh, H. Xiao, M. Chen, and M. I. Cybulsky
Paxillin selectively associates with constitutive and chemoattractant-induced high-affinity {alpha}4{beta}1 integrins: implications for integrin signaling
Blood,
November 1, 2004;
104(9):
2818 - 2824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Rose, S. Liu, D. G. Woodside, J. Han, D. D. Schlaepfer, and M. H. Ginsberg
Paxillin Binding to the {alpha}4 Integrin Subunit Stimulates LFA-1 (Integrin {alpha}L{beta}2)-Dependent T Cell Migration by Augmenting the Activation of Focal Adhesion Kinase/Proline-Rich Tyrosine Kinase-2
J. Immunol.,
June 15, 2003;
170(12):
5912 - 5918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. May, V. Redecke, S. Gruner, R. Schmidt, S. Massberg, T. Miethke, B. Ryba, C. Prazeres da Costa, A. Schomig, and F.-J. Neumann
Recruitment of Chlamydia pneumoniae-Infected Macrophages to the Carotid Artery Wall in Noninfected, Nonatherosclerotic Mice
Arterioscler Thromb Vasc Biol,
May 1, 2003;
23(5):
789 - 794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. May, R. Schmidt, S. M. Kanse, T. Chavakis, R. W. Stephens, A. Schomig, K. T. Preissner, and F.-J. Neumann
Urokinase receptor surface expression regulates monocyte adhesion in acute myocardial infarction
Blood,
November 15, 2002;
100(10):
3611 - 3617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. May, T. Kalsch, S. Massberg, Y. Herouy, R. Schmidt, and M. Gawaz
Engagement of Glycoprotein IIb/IIIa ({alpha}IIb{beta}3) on Platelets Upregulates CD40L and Triggers CD40L-Dependent Matrix Degradation by Endothelial Cells
Circulation,
October 15, 2002;
106(16):
2111 - 2117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Hyduk and M. I. Cybulsky
{alpha}4 Integrin Signaling Activates Phosphatidylinositol 3-Kinase and Stimulates T Cell Adhesion to Intercellular Adhesion Molecule-1 to a Similar Extent As CD3, but Induces a Distinct Rearrangement of the Actin Cytoskeleton
J. Immunol.,
January 15, 2002;
168(2):
696 - 704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Rose, V. Grabovsky, R. Alon, and M. H. Ginsberg
The Affinity of Integrin {alpha}4{beta}1 Governs Lymphocyte Migration
J. Immunol.,
September 1, 2001;
167(5):
2824 - 2830.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Chan, S. J. Hyduk, and M. I. Cybulsky
Chemoattractants Induce a Rapid and Transient Upregulation of Monocyte {alpha}4 Integrin Affinity for Vascular Cell Adhesion Molecule 1 Which Mediates Arrest: An Early Step in the Process of Emigration
J. Exp. Med.,
May 21, 2001;
193(10):
1149 - 1158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Papayannopoulou, G. V. Priestley, B. Nakamoto, V. Zafiropoulos, L. M. Scott, and J. M. Harlan
Synergistic mobilization of hemopoietic progenitor cells using concurrent {beta}1 and {beta}2 integrin blockade or {beta}2-deficient mice
Blood,
March 1, 2001;
97(5):
1282 - 1288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. H. Ulfman, D. P. H. Joosten, J. A. M. van der Linden, J.-W. J. Lammers, J. J. Zwaginga, and L. Koenderman
IL-8 Induces a Transient Arrest of Rolling Eosinophils on Human Endothelial Cells
J. Immunol.,
January 1, 2001;
166(1):
588 - 595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
