|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 2 (July 15), 2000:
pp. 514-522
Different mechanisms define the antiadhesive function of high
molecular weight kininogen in integrin- and urokinase
receptor-dependent interactions
Triantafyllos Chavakis,
Sandip M. Kanse,
Florea Lupu,
Hans-Peter Hammes,
Werner Müller-Esterl,
Robin A. Pixley,
Robert W. Colman, and
Klaus T. Preissner
From the Institute for Biochemistry and the Department of Internal
Medicine, Justus-Liebig-University, Giessen, Germany; Weston Center for
Experimental Research, Thrombosis Research Institute, Manresa Road,
London, England; Institute for Physiological Chemistry,
Johannes-Gutenberg-Universität, Mainz, Germany; and the Sol
Sherry Thrombosis Research Center, Temple University School of
Medicine, Philadelphia, PA.
Proteolytic cleavage of single-chain high molecular weight kininogen
(HK) by kallikrein releases the short-lived vasodilator bradykinin and
leaves behind 2-chain high molecular weight kininogen (HKa) that has
been previously reported to exert antiadhesive properties as well as to
bind to the urokinase receptor (uPAR) on endothelial cells. In this
study we defined the molecular mechanisms for the antiadhesive effects
of HKa related to disruption of integrin- and uPAR-mediated cellular
interactions. Vitronectin (VN) but not fibrinogen or
fibronectin-dependent  v 3 integrin-mediated adhesion
of endothelial cells was blocked by HKa or its isolated domain 5. In a
purified system, HKa but not HK competed for the interaction of VN with
v3 integrin, because HKa and the isolated domain 5 but not HK bound to both multimeric and native VN in a
Zn2+-dependent manner. The interaction between HKa or
domain 5 with VN was prevented by heparin, plasminogen activator
inhibitor-1, and a recombinant glutathione-S-transferase (GST)-fusion
peptide GST-VN (1-77) consisting of the amino terminal portion of VN
(amino acids 1-77), but not by a cyclic arginyl-glycyl-aspartyl
peptide, indicating that HKa interacts with the amino terminal portion of VN ("somatomedin B region"). Furthermore, we have confirmed that HKa but not HK bound to uPAR and to the truncated 2-domain form of
uPAR lacking domain 1 in a Zn2+-dependent manner. Through
these interactions, HKa or its recombinant His-Gly-Lys-rich domain 5 completely inhibited the uPAR-dependent adhesion of myelomonocytic U937
cells and uPAR-transfected BAF-3 cells to VN and thereby promoted cell
detachment. By immunogold electron microscopy, both VN and HK/HKa were
found to be colocalized in sections from human atherosclerotic coronary
artery, indicating that the described interactions are likely to take
place in vivo. Taken together, HK and HKa inhibit different
VN-responsive adhesion receptor systems and may thereby influence
endothelial cell- or leukocyte-related interactions in the vasculature,
particularly under inflammatory conditions.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. A. Queisser, F. M. Kouri, M. Konigshoff, M. Wygrecka, U. Schubert, O. Eickelberg, and K. T. Preissner
Loss of RAGE in Pulmonary Fibrosis: Molecular Relations to Functional Changes in Pulmonary Cell Types
Am. J. Respir. Cell Mol. Biol.,
September 1, 2008;
39(3):
337 - 345.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kitamura, Y. Isaka, Y. Takabatake, R. Imamura, C. Suzuki, S. Takahara, and E. Imai
Nonerythropoietic derivative of erythropoietin protects against tubulointerstitial injury in a unilateral ureteral obstruction model
Nephrol. Dial. Transplant.,
May 1, 2008;
23(5):
1521 - 1528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. J. H. Sachs, C. L. Andrei-Selmer, A. Maniar, T. Weiss, C. Paddock, V. V. Orlova, E. Y. Choi, P. J. Newman, K. T. Preissner, T. Chavakis, et al.
The Neutrophil-specific Antigen CD177 Is a Counter-receptor for Platelet Endothelial Cell Adhesion Molecule-1 (CD31)
J. Biol. Chem.,
August 10, 2007;
282(32):
23603 - 23612.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Sun and K. R. McCrae
Endothelial-cell apoptosis induced by cleaved high-molecular-weight kininogen (HKa) is matrix dependent and requires the generation of reactive oxygen species
Blood,
June 15, 2006;
107(12):
4714 - 4720.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. N. Athanasopoulos, M. Economopoulou, V. V. Orlova, A. Sobke, D. Schneider, H. Weber, H. G. Augustin, S. A. Eming, U. Schubert, T. Linn, et al.
The extracellular adherence protein (Eap) of Staphylococcus aureus inhibits wound healing by interfering with host defense and repair mechanisms
Blood,
April 1, 2006;
107(7):
2720 - 2727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Economopoulou, K. Bdeir, D. B. Cines, F. Fogt, Y. Bdeir, J. Lubkowski, W. Lu, K. T. Preissner, H.-P. Hammes, and T. Chavakis
Inhibition of pathologic retinal neovascularization by {alpha}-defensins
Blood,
December 1, 2005;
106(12):
3831 - 3838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chavakis, A. Athanasopoulos, J.-S. Rhee, V. Orlova, T. Schmidt-Woll, A. Bierhaus, A. E. May, I. Celik, P. P. Nawroth, and K. T. Preissner
Angiostatin is a novel anti-inflammatory factor by inhibiting leukocyte recruitment
Blood,
February 1, 2005;
105(3):
1036 - 1043.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Cao, Y.-L. Guo, and R. W. Colman
Urokinase-Type Plasminogen Activator Receptor Is Involved in Mediating the Apoptotic Effect of Cleaved High Molecular Weight Kininogen in Human Endothelial Cells
Circ. Res.,
May 14, 2004;
94(9):
1227 - 1234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kawasaki, T. Maeda, K. Hanasawa, I. Ohkubo, and T. Tani
Effect of His-Gly-Lys Motif Derived from Domain 5 of High Molecular Weight Kininogen on Suppression of Cancer Metastasis Both in Vitro and in Vivo
J. Biol. Chem.,
December 5, 2003;
278(49):
49301 - 49307.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-B. Michel
Anoikis in the Cardiovascular System: Known and Unknown Extracellular Mediators
Arterioscler Thromb Vasc Biol,
December 1, 2003;
23(12):
2146 - 2154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chavakis, A. Bierhaus, N. Al-Fakhri, D. Schneider, S. Witte, T. Linn, M. Nagashima, J. Morser, B. Arnold, K. T. Preissner, et al.
The Pattern Recognition Receptor (RAGE) Is a Counterreceptor for Leukocyte Integrins: A Novel Pathway for Inflammatory Cell Recruitment
J. Exp. Med.,
November 17, 2003;
198(10):
1507 - 1515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chavakis, S. Santoso, K. J. Clemetson, U. J. H. Sachs, I. Isordia-Salas, R. A. Pixley, P. P. Nawroth, R. W. Colman, and K. T. Preissner
High Molecular Weight Kininogen Regulates Platelet-Leukocyte Interactions by Bridging Mac-1 and Glycoprotein Ib
J. Biol. Chem.,
November 14, 2003;
278(46):
45375 - 45381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zhao, J.-L. Lew, L. Huang, J. Yu, T. Zhang, Y. Hrywna, J. R. Thompson, N. de Pedro, R. A. Blevins, F. Pelaez, et al.
Human Kininogen Gene Is Transactivated by the Farnesoid X Receptor
J. Biol. Chem.,
August 1, 2003;
278(31):
28765 - 28770.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. W. Ng, M. Gutschow, M. Weiss, S. Hauschildt, U. Teubert, T. K. Hecker, F. A. Luzzio, E. A. Kruger, K. Eger, and W. D. Figg
Antiangiogenic Activity of N-substituted and Tetrafluorinated Thalidomide Analogues
Cancer Res.,
June 15, 2003;
63(12):
3189 - 3194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Wang, M. G. Hasham, I. Isordia-Salas, A. Y. Tsygankov, R. W. Colman, and Y.-L. Guo
Regulation of Cardiovascular Signaling by Kinins and Products of Similar Converting Enzyme Systems: Upregulation of Cdc2 and cyclin A during apoptosis of endothelial cells induced by cleaved high-molecular-weight kininogen
Am J Physiol Heart Circ Physiol,
June 1, 2003;
284(6):
H1917 - H1923.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Massimi, E. Park, K. Rice, W. Muller-Esterl, D. Sauder, and M. J. McGavin
Identification of a Novel Maturation Mechanism and Restricted Substrate Specificity for the SspB Cysteine Protease of Staphylococcus aureus
J. Biol. Chem.,
October 25, 2002;
277(44):
41770 - 41777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. Zhang, F. Donate, X. Qi, N. P. Ziats, J. C. Juarez, A. P. Mazar, Y.-P. Pang, and K. R. McCrae
The antiangiogenic activity of cleaved high molecular weight kininogen is mediated through binding to endothelial cell tropomyosin
PNAS,
September 17, 2002;
99(19):
12224 - 12229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Santoso, U. J.H. Sachs, H. Kroll, M. Linder, A. Ruf, K. T. Preissner, and T. Chavakis
The Junctional Adhesion Molecule 3 (JAM-3) on Human Platelets is a Counterreceptor for the Leukocyte Integrin Mac-1
J. Exp. Med.,
September 2, 2002;
196(5):
679 - 691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chavakis, R. A. Pixley, I. Isordia-Salas, R. W. Colman, and K. T. Preissner
A Novel Antithrombotic Role for High Molecular Weight Kininogen as Inhibitor of Plasminogen Activator Inhibitor-1 Function
J. Biol. Chem.,
August 30, 2002;
277(36):
32677 - 32682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chavakis, N. Boeckel, S. Santoso, R. Voss, I. Isordia-Salas, R. A. Pixley, E. Morgenstern, R. W. Colman, and K. T. Preissner
Inhibition of Platelet Adhesion and Aggregation by a Defined Region (Gly-486-Lys-502) of High Molecular Weight Kininogen
J. Biol. Chem.,
June 21, 2002;
277(26):
23157 - 23164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. CHAVAKIS, S. M. KANSE, R. A. PIXLEY, A. E. MAY, I. ISORDIA-SALAS, R. W. COLMAN, and K. T. PREISSNER
Regulation of leukocyte recruitment by polypeptides derived from high molecular weight kininogen
FASEB J,
November 1, 2001;
15(13):
2365 - 2376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-L. Guo, S. Wang, and R. W. Colman
Kininostatin, an Angiogenic Inhibitor, Inhibits Proliferation and Induces Apoptosis of Human Endothelial Cells
Arterioscler Thromb Vasc Biol,
September 1, 2001;
21(9):
1427 - 1433.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. D. Liang, T. Chavakis, S. M. Kanse, and K. T. Preissner
Ligand Binding Regions in the Receptor for Urokinase-type Plasminogen Activator. STRUCTURAL REQUIREMENTS FOR A MULTIDOMAIN BINDING REGION AND RECEPTOR-RECEPTOR INTERACTION
J. Biol. Chem.,
July 27, 2001;
276(31):
28946 - 28953.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
