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Blood, Vol. 96 No. 2 (July 15), 2000:
pp. 771-772
BRIEF REPORT
Immunohistochemical staining for megakaryocyte c-mpl may
complement morphologic distinction between polycythemia vera and
secondary erythrocytosis
Ayalew Tefferi,
Soo-Young Yoon, and
Chin-Yang Li
From the Division of Hematology and Internal Medicine and the
Division of Hematopathology, Mayo Clinic and Mayo Foundation,
Rochester, MN.
Recent studies have shown decreased megakaryocyte expression of the
thrombopoietin receptor (c-mpl) in patients with polycythemia vera (PV) but not in those with reactive erythrocytosis. We examined the diagnostic utility of this observation in 22 patients with PV, 7 patients with secondary erythrocytosis (SE), and 10 normal controls.
Commercial antibodies against c-mpl were used with standard immunoperoxidase methods. Megakaryocyte c-mpl staining
intensity was uniformly moderate-to-strong in the healthy controls and
in all the patients with SE. In contrast, staining intensity in 9 patients with PV (41%) was uniformly weak. Furthermore, in 12 of the
remaining 13 patients with PV, the c-mpl staining pattern in
each case was heterogeneous and was associated with weak staining intensity in more than 20% of the megakaryocyte population. These preliminary data suggest that c-mpl immunostains may complement bone marrow histopathology in distinguishing PV from nonclonal causes
of erythrocytosis.
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