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Related Collections Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 August 2000, Vol. 96, No. 4, pp. 1505-1511 NEOPLASIA High incidence of chromosome 13 deletion in multiple myeloma detected by multiprobe interphase FISH John Shaughnessy Jr, Erming Tian, Jeffrey Sawyer, Klaus Bumm, Reid Landes, Ashraf Badros, Christopher Morris, Guido Tricot, Joshua Epstein, and Bart Barlogie From the Myeloma and Transplantation Research Center, Division of Biometry, University of Arkansas for Medical Sciences and Arkansas Cancer Research Center, Little Rock, AR Multiple myeloma (MM) is a hypoproliferative malignancy yielding informative karyotypes in no more than 30% of newly diagnosed cases. Although cytogenetic and molecular deletion of chromosome 13 is associated with poor prognosis, a MM tumor suppressor gene (TSG) has not been identified. To localize a minimal deleted region of chromosome 13, clonotypic plasma cells from 50 consecutive patients with MM were subjected to interphase fluorescence in situ hybridization (FISH) analysis using a panel of 11 probes spanning the entire long arm of chromosome 13. Whereas chromosome 13 abnormalities were absent in plasma cells from 25 normal donors, 86% of patients with MM demonstrated such aberrations. Heterogeneity, both in deletion frequency and extent, was confirmed by simultaneous FISH with 2 chromosome 13 probes. Deletion hot spots were noted at D13S272 (70%) and D13S31 (64%), 2 unlinked loci at 13q14. Homozygous deletions at these loci occurred in 12% (simultaneously in 8%) of the cases. Molecular deletions were found in all 14 patients with morphologic deletions, in 21 of 24 with uninformative karyotypes, and 8 of 12 patients with karyotype abnormalities lacking chromosome 13 deletion. Homozygous deletion of any marker was noted in 4% with low and in 36% with higher plasma cell labeling index greater than 0.4% (P = .01). The absence of increasing deletion incidence and extent with therapy duration suggests that the observed lesions are not induced by treatment. The high incidence and extent of chromosome 13 deletions require the correlation of specific deletion(s) with poor prognosis. These analyses will provide valuable guidance toward cloning of an MM-TSG. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Hanlon, L. W. Harries, S. Ellard, and C. E. Rudin Evaluation of 13q14 Status in Multiple Myeloma by Digital Single Nucleotide Polymorphism Technology J. Mol. Diagn., September 1, 2009; 11(5): 450 - 457. [Abstract] [Full Text] [PDF] M. A. Shammas, R. J. Shmookler Reis, H. Koley, R. B. Batchu, C. Li, and N. C. 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