|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2000, Vol. 96, No. 4, pp. 1596-1598
BRIEF REPORT
Human erythrocyte pyrimidine 5'-nucleotidase, PN-I, is
identical to p36, a protein associated to lupus inclusion formation in
response to  -interferon
Adolfo Amici,
Monica Emanuelli,
Nadia Raffaelli,
Silverio Ruggieri,
Franca Saccucci, and
Giulio Magni
From the Istituto di Biochimica and Dipartimento di
Biotecnologie Agrarie ed Ambientali, Università di Ancona,
Ancona, Italy.
Erythrocyte maturation is accompanied by RNA degradation and
release of mononucleotides. We have previously purified PN-I, a
pyrimidine nucleotidase whose deficiency is associated with hemolytic
anemia. Computer-aided analysis of PN-I tryptic and CNBr peptide
sequences revealed substantial identity with tryptic peptide sequences
reported for p36, an  -interferon-induced protein. PN-I partial
sequences were matched through the expressed sequence tag database with
different human complementary DNA (cDNA) clones, whose sequences were
exploited to screen a human placenta cDNA library. PN-I cDNA, coding
for a 286-residue protein, was expressed in Escherichia
coli, yielding a fully active recombinant enzyme. The recombinant
protein sequence comprised the peptide sequences determined for PN-I
and p36. Rabbit antisera raised against two peptides deriving from p36
and PN-I tryptic digestions, respectively, recognized both wild-type
and recombinant PN-I. Molecular properties of the two proteins were
essentially the same. We conclude that p36 and PN-I are identical proteins.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. R. Chiarelli, P. Bianchi, E. Fermo, A. Galizzi, P. Iadarola, A. Mattevi, A. Zanella, and G. Valentini
Functional analysis of pyrimidine 5'-nucleotidase mutants causing nonspherocytic hemolytic anemia
Blood,
April 15, 2005;
105(8):
3340 - 3345.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mass, E. Simo, and S. Dragon
Erythroid pyrimidine 5'-nucleotidase: cloning, developmental expression, and regulation by cAMP and in vivo hypoxia
Blood,
December 1, 2003;
102(12):
4198 - 4205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Bianchi and J. Spychala
Mammalian 5'-Nucleotidases
J. Biol. Chem.,
November 21, 2003;
278(47):
46195 - 46198.
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Balta, F. Gumruk, N. Akarsu, A. Gurgey, and C. Altay
Molecular characterization of Turkish patients with pyrimidine 5' nucleotidase-I deficiency
Blood,
September 1, 2003;
102(5):
1900 - 1903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Gallinaro, K. Crovatto, C. Rampazzo, G. Pontarin, P. Ferraro, E. Milanesi, P. Reichard, and V. Bianchi
Human Mitochondrial 5'-Deoxyribonucleotidase. OVERPRODUCTION IN CULTURED CELLS AND FUNCTIONAL ASPECTS
J. Biol. Chem.,
September 13, 2002;
277(38):
35080 - 35087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Medh
Microarray-Based Expression Profiling of Normal and Malignant Immune Cells
Endocr. Rev.,
June 1, 2002;
23(3):
393 - 400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Marinaki, E. Escuredo, J. A. Duley, H. A. Simmonds, A. Amici, V. Naponelli, G. Magni, M. Seip, I. Ben-Bassat, E. H. Harley, et al.
Genetic basis of hemolytic anemia caused by pyrimidine 5' nucleotidase deficiency
Blood,
June 1, 2001;
97(11):
3327 - 3332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Allegrini, A. Scaloni, L. Ferrara, R. Pesi, P. Pinna, F. Sgarrella, M. Camici, S. Eriksson, and M. G. Tozzi
Bovine Cytosolic 5'-Nucleotidase Acts through the Formation of an Aspartate 52-Phosphoenzyme Intermediate
J. Biol. Chem.,
August 31, 2001;
276(36):
33526 - 33532.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
