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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Merad, M. Articles by Engleman, E. G. Search for Related Content PubMed PubMed Citation Articles by Merad, M. Articles by Engleman, E. G. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 September 2000, Vol. 96, No. 5, pp. 1865-1872 IMMUNOBIOLOGY Differentiation of myeloid dendritic cells into CD8-positive dendritic cells in vivo Miriam Merad, Lawrence Fong, Jakob Bogenberger, and Edgar G. Engleman From the Department of Pathology, Stanford University School of Medicine, Stanford, CA. Bone marrow-derived dendritic cells (DC) represent a family of antigen-presenting cells (APC) with varying phenotypes. For example, in mice, CD8+ and CD8 DC are thought to represent cells of lymphoid and myeloid origin, respectively. Langerhans cells (LC) of the epidermis are typical myeloid DC; they do not express CD8, but they do express high levels of myeloid antigens such as CD11b and FcR. By contrast, thymic DC, which derive from a lymphoid-related progenitor, express CD8 but only low levels of myeloid antigens. CD8+ DC are also found in the spleen and lymph nodes (LN), but the origin of these cells has not been determined. By activating and labeling CD8 epidermal LC in vivo, it was found that these cells expressed CD8 on migration to the draining LN. Similarly, CD8 LC generated in vitro from a CD8 wild-type mouse and injected into the skin of a CD8KO mouse expressed CD8 when they reached the draining LN. The results also show that CD8+ LC are potent APC. After migration from skin, they localized in the T-cell areas of LN, secreted high levels of interleukin-12, interferon-, and chemokine-attracting T cells, and they induced antigen-specific T-cell activation. These results demonstrate that myeloid DC in the periphery can express CD8 when they migrate to the draining LN. CD8 expression on these DC appears to reflect a state of activation, mobilization, or both, rather than lineage. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. K. Lee, M. Zamora, M. M. Linehan, N. Iijima, D. Gonzalez, A. Haberman, and A. 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