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Blood, 1 September 2000, Vol. 96, No. 5, pp. 1989-1993
TRANSPLANTATION
CD34 expression by murine hematopoietic stem cells mobilized
by granulocyte colony-stimulating factor
Fumihito Tajima,
Takashi Sato,
Joseph H. Laver, and
Makio Ogawa
From the Departments of Medicine and Pediatrics,
Medical University of South Carolina, and the Department of Veterans
Affairs Medical Center, Charleston, SC.
Controversy has existed about CD34 expression by hematopoietic stem
cells. We recently reported that CD34 expression reflects the
activation state of stem cells by using a murine transplantation model.
It has been generally held that mobilized blood stem cells express
CD34.However, it has also been reported that mobilized stem cells and
progenitors are in G0/G1 phases of the cell cycle. To address the state
of CD34 expression by the mobilized stem cells, we again used the mouse
transplantation model. We prepared CD34 and
CD34+ populations of nucleated blood cells from granulocyte
colony-stimulating factor-treated Ly-5.1 mice and assayed each
population for long-term engrafting cells in lethally irradiated Ly-5.2
mice. The majority of the stem cells were in the CD34+
population. The CD34 expression by mobilized stem cells was reversible because re-transplantation of Ly-5.1 CD34 marrow cells
harvested from the Ly-5.2 recipients of CD34+-mobilized
stem cells 8 months posttransplantation revealed long-term engraftment.
These results may support the use of total CD34+ cells in
mobilized blood as a predictor for engraftment and CD34 selection for
enrichment of human stem cells.
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