|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 October 2000, Vol. 96, No. 7, pp. 2460-2468
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Sulfated glycans induce rapid hematopoietic progenitor cell
mobilization: evidence for selectin-dependent and independent
mechanisms
Paul S. Frenette and
Linnea Weiss
From the Mount Sinai School of Medicine, Department of
Medicine, New York, NY.
The adhesive mechanisms leading to the mobilization of
hematopoietic progenitor cells (HPCs) from the bone marrow into the blood are poorly understood. We report on a role for selectins and
fucoidan in progenitor mobilization. Baseline levels of circulating HPCs are increased in endothelial selectin-deficient (P/E /) mice.
Similar levels are observed when E-selectin null (E/) mice are
treated with anti-P-selectin antibody or with fucoidan (which inhibits
P- and L-selectin function). In particular, administration of 2 doses
of fucoidan (25 mg/kg) over 6 hours produces profound mobilization of
progenitors in wild-type mice and the response is greatly enhanced in
E/ and P/E/ mice. Competitive reconstitution experiments reveal
that fucoidan also elicits long-term (more than 6 months) repopulating
stem cells. Mobilization assays using chimeric mice harboring
L-selectin-deficient progenitors and wild-type progenitors expressing
the green fluorescence protein suggest that L-selectin expression is
not required but confers an advantage for fucoidan-induced
mobilization. Sulfation is critical as desulfated fucoidan is
ineffective. In addition, sulphogalactosylceramide (sulfatide) but not
heparin can induce HPC mobilization. Our results indicate that
administration of sulfated glycans, especially with concurrent
inhibition of E-selectin function, represents a powerful novel method
for rapid mobilization of long-term-repopulating stem cells. These
findings may help elucidate the mechanisms of HPC trafficking during
development and adult life.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. Lucchinetti, S. M. Zeisberger, I. Baruscotti, J. Wacker, J. Feng, K. Zaugg, R. Dubey, A. H. Zisch, and M. Zaugg
Stem Cell-Like Human Endothelial Progenitors Show Enhanced Colony-Forming Capacity After Brief Sevoflurane Exposure: Preconditioning of Angiogenic Cells by Volatile Anesthetics
Anesth. Analg.,
October 1, 2009;
109(4):
1117 - 1126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Prasad, D. Lillicrap, A. Labelle, S. Knappe, T. Keller, E. Burnett, S. Powell, and K. W. Johnson
Efficacy and safety of a new-class hemostatic drug candidate, AV513, in dogs with hemophilia A
Blood,
January 15, 2008;
111(2):
672 - 679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takeda and M. S. Patel
Neural Control of Hematopoietic Stem Cell Mobilization via Osteoblasts
IBMS BoneKEy,
May 1, 2006;
3(5):
39 - 41.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Barrabes, D. Garcia-Dorado, M. Mirabet, J. Inserte, L. Agullo, B. Soriano, A. Massaguer, F. Padilla, R.-M. Lidon, and J. Soler-Soler
Antagonism of selectin function attenuates microvascular platelet deposition and platelet-mediated myocardial injury after transient ischemia
J. Am. Coll. Cardiol.,
January 18, 2005;
45(2):
293 - 299.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Monfardini, V. Van Merris, M. Paape, L. Duchateau, and C. Burvenich
L-Selectin and Chemotaxis Throughout Bone Marrow Granulocyte Maturation in the Bovine
J Dairy Sci,
October 1, 2004;
87(10):
3350 - 3357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hidalgo, A. J. Peired, L. A. Weiss, Y. Katayama, and P. S. Frenette
The integrin {alpha}M{beta}2 anchors hematopoietic progenitors in the bone marrow during enforced mobilization
Blood,
August 15, 2004;
104(4):
993 - 1001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Pelus, H. Bian, A. G. King, and S. Fukuda
Neutrophil-derived MMP-9 mediates synergistic mobilization of hematopoietic stem and progenitor cells by the combination of G-CSF and the chemokines GRO{beta}/CXCL2 and GRO{beta}T /CXCL2{Delta}4
Blood,
January 1, 2004;
103(1):
110 - 119.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Netelenbos, J. van den Born, F. L. Kessler, S. Zweegman, P. C. Huijgens, and A. M. Drager
In vitro model for hematopoietic progenitor cell homing reveals endothelial heparan sulfate proteoglycans as direct adhesive ligands
J. Leukoc. Biol.,
December 1, 2003;
74(6):
1035 - 1044.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
O. Berteau and B. Mulloy
Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide
Glycobiology,
June 1, 2003;
13(6):
29R - 40R.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-E. Luyt, A. Meddahi-Pelle, B. Ho-Tin-Noe, S. Colliec-Jouault, J. Guezennec, L. Louedec, H. Prats, M.-P. Jacob, M. Osborne-Pellegrin, D. Letourneur, et al.
Low-Molecular-Weight Fucoidan Promotes Therapeutic Revascularization in a Rat Model of Critical Hindlimb Ischemia
J. Pharmacol. Exp. Ther.,
April 1, 2003;
305(1):
24 - 30.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Carlo-Stella, M. Di Nicola, M. Magni, P. Longoni, M. Milanesi, C. Stucchi, L. Cleris, F. Formelli, and M. A. Gianni
Defibrotide in Combination with Granulocyte Colony-stimulating Factor Significantly Enhances the Mobilization of Primitive and Committed Peripheral Blood Progenitor Cells in Mice
Cancer Res.,
November 1, 2002;
62(21):
6152 - 6157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Deux, A. Meddahi-Pelle, A. F. Le Blanche, L. J. Feldman, S. Colliec-Jouault, F. Bree, F. Boudghene, J.-B. Michel, and D. Letourneur
Low Molecular Weight Fucoidan Prevents Neointimal Hyperplasia in Rabbit Iliac Artery In-Stent Restenosis Model
Arterioscler Thromb Vasc Biol,
October 1, 2002;
22(10):
1604 - 1609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Sweeney, H. Lortat-Jacob, G. V. Priestley, B. Nakamoto, and T. Papayannopoulou
Sulfated polysaccharides increase plasma levels of SDF-1 in monkeys and mice: involvement in mobilization of stem/progenitor cells
Blood,
January 1, 2002;
99(1):
44 - 51.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kollet, A. Spiegel, A. Peled, I. Petit, T. Byk, R. Hershkoviz, E. Guetta, G. Barkai, A. Nagler, and T. Lapidot
Rapid and efficient homing of human CD34+CD38{-}/lowCXCR4+ stem and progenitor cells to the bone marrow and spleen of NOD/SCID and NOD/SCID/B2mnull mice
Blood,
May 15, 2001;
97(10):
3283 - 3291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
