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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Keller, S. A. Articles by Cesarman, E. Search for Related Content PubMed PubMed Citation Articles by Keller, S. A. Articles by Cesarman, E. Related Collections Neoplasia Apoptosis Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 October 2000, Vol. 96, No. 7, pp. 2537-2542 NEOPLASIA Inhibition of NF-B induces apoptosis of KSHV-infected primary effusion lymphoma cells Shannon A. Keller, Elaine J. Schattner, and Ethel Cesarman From the Weill Graduate School of Medical Sciences of Cornell University, and the Departments of Medicine and Pathology of Weill Medical College of Cornell University, New York, NY. Kaposi sarcoma-associated herpesvirus (KSHV), or human herpervirus 8 (HHV-8), is a -herpesvirus that infects human lymphocytes and is associated with primary effusion lymphoma (PEL). Currently, the role of viral infection in the transformation of PEL cells is unknown. One possibility is that KSHV, like the lymphotropic viruses Epstein-Barr virus (EBV) and human T-cell leukemia virus I (HTLV-I), activates the transcription factor NF-B to promote survival and proliferation of infected lymphocytes. To examine this possibility, we assessed NF-B activity in KSHV-infected PEL cell lines and primary tumor specimens by electrophoretic mobility shift assay (EMSA). We observed that NF-B is constitutively activated in all KSHV-infected lymphomas, and consists of 2 predominant complexes, p65/p50 heterodimers and p50/p50 homodimers. Inhibition experiments demonstrated that Bay 11-7082, an irreversible inhibitor of IB phosphorylation, completely and specifically abrogated the NF-B/DNA binding in PEL cells. PEL cells treated with Bay 11 demonstrated down-regulation of the NF-B inducible cytokine interleukin 6 (IL-6), and apoptosis. These results suggest that NF-B activity is necessary for survival of KSHV-infected lymphoma cells, and that pharmacologic inhibition of NF-B may be an effective treatment for PEL. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Duchene, C. Cayla, S. Vessillier, R. Scotland, K. Yamashiro, F. Lecomte, I. Syed, P. Vo, A. Marrelli, C. Pitzalis, et al. 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Cohen, J. Gill, G. Farr, S. Wang, et al. Activation of Src kinase Lyn by the Kaposi sarcoma-associated herpesvirus K1 protein: implications for lymphomagenesis Blood, May 15, 2005; 105(10): 3987 - 3994. [Abstract] [Full Text] [PDF] A. Godfrey, J. Anderson, A. Papanastasiou, Y. Takeuchi, and C. Boshoff Inhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA Blood, March 15, 2005; 105(6): 2510 - 2518. [Abstract] [Full Text] [PDF] A. R. Jazirehi, S. Huerta-Yepez, G. Cheng, and B. Bonavida Rituximab (Chimeric Anti-CD20 Monoclonal Antibody) Inhibits the Constitutive Nuclear Factor-{kappa}B Signaling Pathway in Non-Hodgkin's Lymphoma B-Cell Lines: Role in Sensitization to Chemotherapeutic Drug-induced Apoptosis Cancer Res., January 1, 2005; 65(1): 264 - 276. [Abstract] [Full Text] [PDF] L. T. Lam, R. E. Davis, J. Pierce, M. Hepperle, Y. Xu, M. Hottelet, Y. Nong, D. Wen, J. Adams, L. Dang, et al. Small Molecule Inhibitors of I{kappa}B Kinase Are Selectively Toxic for Subgroups of Diffuse Large B-Cell Lymphoma Defined by Gene Expression Profiling Clin. Cancer Res., January 1, 2005; 11(1): 28 - 40. [Abstract] [Full Text] [PDF] T. Luft, E. Maraskovsky, M. Schnurr, K. Knebel, M. Kirsch, M. Gorner, R. Skoda, A. D. Ho, P. Nawroth, and A. Bierhaus Tuning the volume of the immune response: strength and persistence of stimulation determine migration and cytokine secretion of dendritic cells Blood, August 15, 2004; 104(4): 1066 - 1074. [Abstract] [Full Text] [PDF] H. Matta and P. M. Chaudhary Activation of alternative NF-{kappa}B pathway by human herpes virus 8-encoded Fas-associated death domain-like IL-1{beta}-converting enzyme inhibitory protein (vFLIP) PNAS, June 22, 2004; 101(25): 9399 - 9404. [Abstract] [Full Text] [PDF] E. D. Cahir-McFarland, K. Carter, A. Rosenwald, J. M. Giltnane, S. E. Henrickson, L. M. Staudt, and E. Kieff Role of NF-{kappa}B in Cell Survival and Transcription of Latent Membrane Protein 1-Expressing or Epstein-Barr Virus Latency III-Infected Cells J. Virol., April 15, 2004; 78(8): 4108 - 4119. [Abstract] [Full Text] [PDF] I. Guasparri, S. A. Keller, and E. Cesarman KSHV vFLIP Is Essential for the Survival of Infected Lymphoma Cells J. Exp. Med., April 5, 2004; 199(7): 993 - 1003. [Abstract] [Full Text] [PDF] Y. Dai, X.-Y. Pei, M. Rahmani, D. H. Conrad, P. Dent, and S. Grant Interruption of the NF-{kappa}B pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells Blood, April 1, 2004; 103(7): 2761 - 2770. [Abstract] [Full Text] [PDF] Q. Sun, S. Zachariah, and P. M. Chaudhary The Human Herpes Virus 8-Encoded Viral FLICE-inhibitory Protein Induces Cellular Transformation via NF-{kappa}B Activation J. Biol. Chem., December 26, 2003; 278(52): 52437 - 52445. [Abstract] [Full Text] [PDF] S. Shi, C. Nathan, D. Schnappinger, J. Drenkow, M. Fuortes, E. Block, A. Ding, T. R. Gingeras, G. Schoolnik, S. Akira, et al. MyD88 Primes Macrophages for Full-Scale Activation by Interferon-{gamma} yet Mediates Few Responses to Mycobacterium tuberculosis J. Exp. Med., October 6, 2003; 198(7): 987 - 997. [Abstract] [Full Text] [PDF] N. Field, W. Low, M. Daniels, S. Howell, L. Daviet, C. Boshoff, and M. Collins KSHV vFLIP binds to IKK-{gamma} to activate IKK J. Cell Sci., September 15, 2003; 116(18): 3721 - 3728. [Abstract] [Full Text] [PDF] H. J. Brown, M. J. Song, H. Deng, T.-T. Wu, G. Cheng, and R. Sun NF-{kappa}B Inhibits Gammaherpesvirus Lytic Replication J. Virol., August 1, 2003; 77(15): 8532 - 8540. [Abstract] [Full Text] [PDF] L. A. Dourmishev, A. L. Dourmishev, D. Palmeri, R. A. Schwartz, and D. M. Lukac Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 175 - 212. [Abstract] [Full Text] [PDF] M. Z. Dewan, K. Terashima, M. Taruishi, H. Hasegawa, M. Ito, Y. Tanaka, N. Mori, T. Sata, Y. Koyanagi, M. Maeda, et al. Rapid Tumor Formation of Human T-Cell Leukemia Virus Type 1-Infected Cell Lines in Novel NOD-SCID/{gamma}cnull Mice: Suppression by an Inhibitor against NF-{kappa}B J. Virol., May 1, 2003; 77(9): 5286 - 5294. [Abstract] [Full Text] [PDF] S. K. Ghosh, C. Wood, L. H. Boise, A. M. Mian, V. V. Deyev, G. Feuer, N. L. Toomey, N. C. Shank, L. Cabral, G. N. Barber, et al. Potentiation of TRAIL-induced apoptosis in primary effusion lymphoma through azidothymidine-mediated inhibition of NF-kappa B Blood, March 15, 2003; 101(6): 2321 - 2327. [Abstract] [Full Text] [PDF] Q. Sun, H. Matta, and P. M. Chaudhary The human herpes virus 8-encoded viral FLICE inhibitory protein protects against growth factor withdrawal-induced apoptosis via NF-kappa B activation Blood, March 1, 2003; 101(5): 1956 - 1961. [Abstract] [Full Text] [PDF] M. Cannon, N. J. Philpott, and E. Cesarman The Kaposi's Sarcoma-Associated Herpesvirus G Protein-Coupled Receptor Has Broad Signaling Effects in Primary Effusion Lymphoma Cells J. Virol., December 6, 2002; 77(1): 57 - 67. [Abstract] [Full Text] [PDF] O. Gutierrez, C. Pipaon, N. Inohara, A. Fontalba, Y. Ogura, F. Prosper, G. Nunez, and J. L. Fernandez-Luna Induction of Nod2 in Myelomonocytic and Intestinal Epithelial Cells via Nuclear Factor-kappa B Activation J. Biol. Chem., October 25, 2002; 277(44): 41701 - 41705. [Abstract] [Full Text] [PDF] N. Mori, Y. Yamada, S. Ikeda, Y. Yamasaki, K. Tsukasaki, Y. Tanaka, M. Tomonaga, N. Yamamoto, and M. Fujii Bay 11-7082 inhibits transcription factor NF-kappa B and induces apoptosis of HTLV-I-infected T-cell lines and primary adult T-cell leukemia cells Blood, August 13, 2002; 100(5): 1828 - 1834. [Abstract] [Full Text] [PDF] O. Prakash, Z.-Y. Tang, X. Peng, R. Coleman, J. Gill, G. Farr, and F. Samaniego Tumorigenesis and Aberrant Signaling in Transgenic Mice Expressing the Human Herpesvirus-8 K1 Gene J Natl Cancer Inst, June 19, 2002; 94(12): 926 - 935. [Abstract] [Full Text] [PDF] T. Hideshima, D. Chauhan, P. Richardson, C. Mitsiades, N. Mitsiades, T. Hayashi, N. Munshi, L. Dang, A. Castro, V. Palombella, et al. NF-kappa B as a Therapeutic Target in Multiple Myeloma J. Biol. Chem., May 3, 2002; 277(19): 16639 - 16647. [Abstract] [Full Text] [PDF] L. Liu, M. T. Eby, N. Rathore, S. K. Sinha, A. Kumar, and P. M. Chaudhary The Human Herpes Virus 8-encoded Viral FLICE Inhibitory Protein Physically Associates with and Persistently Activates the Ikappa B Kinase Complex J. Biol. Chem., April 12, 2002; 277(16): 13745 - 13751. [Abstract] [Full Text] [PDF] T. V. Sharp, H.-W. Wang, A. Koumi, D. Hollyman, Y. Endo, H. Ye, M.-Q. Du, and C. Boshoff K15 Protein of Kaposi's Sarcoma-Associated Herpesvirus Is Latently Expressed and Binds to HAX-1, a Protein with Antiapoptotic Function J. Virol., January 15, 2002; 76(2): 802 - 816. [Abstract] [Full Text] [PDF] J. An, A. K. Lichtenstein, G. Brent, and M. B. Rettig The Kaposi sarcoma-associated herpesvirus (KSHV) induces cellular interleukin 6 expression: role of the KSHV latency-associated nuclear antigen and the AP1 response element Blood, January 15, 2002; 99(2): 649 - 654. [Abstract] [Full Text] [PDF] S. Pati, M. Cavrois, H.-G. Guo, J. S. Foulke Jr., J. Kim, R. A. Feldman, and M. Reitz Activation of NF-{kappa}B by the Human Herpesvirus 8 Chemokine Receptor ORF74: Evidence for a Paracrine Model of Kaposi's Sarcoma Pathogenesis J. Virol., September 15, 2001; 75(18): 8660 - 8673. [Abstract] [Full Text] [PDF] S. Montaner, A. Sodhi, S. Pece, E. A. Mesri, and J. S. Gutkind The Kaposi's Sarcoma-associated Herpesvirus G Protein-coupled Receptor Promotes Endothelial Cell Survival through the Activation of Akt/Protein Kinase B Cancer Res., March 1, 2001; 61(6): 2641 - 2648. [Abstract] [Full Text]

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