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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Berlanga, O. Articles by Watson, S. P. Search for Related Content PubMed PubMed Citation Articles by Berlanga, O. Articles by Watson, S. P. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 October 2000, Vol. 96, No. 8, pp. 2740-2745 HEMATOPOIESIS Expression of the collagen receptor glycoprotein VI during megakaryocyte differentiation Oscar Berlanga, Regis Bobe, Marion Becker, George Murphy, Mireille Leduc, Cassian Bon, Fiona A. Barry, Jonathan M. Gibbins, Paloma Garcia, Jonathan Frampton, and Stephen P. Watson From the Department of Pharmacology, University of Oxford, United Kingdom; Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom; Unité des venins, Institut Pasteur, Paris, France; School of Animal and Microbial Sciences, University of Reading, Reading, United Kingdom. This study examined the expression of the platelet collagen receptor glycoprotein VI (GPVI) in megakaryocyte cell lines and primary megakaryocytes by reverse transcriptase-polymerase chain reaction and by flow cytometry and ligand blotting using the snake venom toxin convulxin. Expression of GPVI is increased in the megakaryoblastic cell lines HEL and CMK on differentiation with the phorbol ester phorbol 12-myristate 13-acetate (PMA), along with the Fc receptor -chain (FcR -chain). The increase in GPVI expression is associated with marked potentiation of tyrosine phosphorylation and Ca++ elevation in response to convulxin. Syk, linker for activated T cells, and phospholipase C2 (PLC2) are among the proteins tyrosine phosphorylated on convulxin stimulation in PMA-differentiated HEL cells. Studies on primary murine megakaryocytes grown in vitro confirmed that GPVI is up-regulated in parallel with functional activation, assessed by measurement of [Ca++]i, during differentiation. The results demonstrate that expression of GPVI is up-regulated along with the FcR -chain during differentiation of megakaryocytes. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Z. Zou, A. A. Schmaier, L. Cheng, P. Mericko, S. K. Dickeson, T. P. Stricker, S. A. Santoro, and M. L. 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