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Blood, 1 June 2001, Vol. 97, No. 11, pp. 3470-3477
IMMUNOBIOLOGY
Investigation of human spleen dendritic cell phenotype and
distribution reveals evidence of in vivo activation in a subset of
organ donors
Dorian McIlroy,
Christelle Troadec,
Fernanda Grassi,
Assia Samri,
Benoît Barrou,
Brigitte Autran,
Patrice Debré,
Jean Feuillard, and
Anne Hosmalin
From the Laboratoire d'Immunologie Cellulaire et
Tissulaire URA CNRS 625, Service d'Urologie, Hôpital de La
Pitié-Salpêtrière, Paris; and Service
d'Hématologie biologique, Hôpital Avicenne, Bobigny,
France.
Although the mouse spleen dendritic cell (DC) is perhaps the most
intensively studied DC type, little has been published concerning its
human equivalent. In this report, rare event flow cytometry and in situ
immunofluorescence were used to study the surface phenotype and
distribution of HLA-DR+
CD3 141619 human
spleen DC. Spleens from organ donors with different clinical histories
were used. Most (81% ± 9%; n = 14) spleen DCs expressed high
levels of the integrin CD11c. CD11c+ DCs were distributed
in 3 distinct regions the peri-arteriolar T-cell zones, the B-cell
zones, and the marginal zone, where they formed a ring of cells
surrounding the white pulp, just inside a ring of CD14+ red
pulp macrophages, apparently more regularly organized than the
previously described marginating DC population in the mouse spleen. The
T-cell zones contained CD86+ DCs, among which a
subpopulation expressed CD83. These mature/activated CD86+
DCs represented a minority (12% ± 8%) of total spleen DCs in most
organ donors: most spleen DCs are immature. In 3 of 18 (17%) donors,
however, most (54%-81%) of spleen DCs were CD86+,
suggesting that in vivo DC activation had occurred. In one donor, a
radical shift in DC distribution from the marginal zone to the T-cell
zones was also observed. This activation of spleen DCs in vivo was
reminiscent of the effects of experimental microbial product injection
in mice, and it seemed to correlate with bacterial infection or
multiple trauma.
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