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Blood, 1 June 2001, Vol. 97, No. 11, pp. 3655-3657

BRIEF REPORT

Arrest of human dendritic cells at the CD34minus /CD4+/HLA-DR+ stage in the bone marrow of NOD/SCID-human chimeric mice

Masaharu Nobuyoshi, Yoichiro Kusunoki, Toshio Seyama, Kazunori Kodama, Akiro Kimura, and Seishi Kyoizumi

From the Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima and Nagasaki, Japan.

Human dendritic cell (DC) precursors were engrafted and maintained in NOD/SCID- human chimeric mice (NOD/SCID-hu mice) implanted with human cord blood mononuclear cells, although no mature human DCs were detected in lymphoid organs of the mice. Two months after implantation, bone marrow (BM) cells of NOD/SCID-hu mice formed colonies showing DC morphology and expressing CD1a in methylcellulose culture with granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor alpha  (TNF-alpha ). The CD34-/CD4+/HLA-DR+ cell fraction in NOD/SCID-hu mouse BM generated CD1a+ cells that were highly stimulatory in mixed leukocyte reactions in culture with GM-CSF and TNF-alpha . These results suggest a strong potential for NOD/SCID-hu BM to generate human DCs, although DC differentiation may be blocked at the CD34-/CD4+/HLA-DR+ stage.

© 2001 by The American Society of Hematology.
 

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