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Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 January 2001, Vol. 97, No. 2, pp. 419-425 HEMATOPOIESIS CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages Sahoko Matsuoka, Yasuhiro Ebihara, Ming-jiang Xu, Takefumi Ishii, Daisuke Sugiyama, Hiroshi Yoshino, Takahiro Ueda, Atsushi Manabe, Ryuhei Tanaka, Yasuo Ikeda, Tatsutoshi Nakahata, and Kohichiro Tsuji From the Department of Clinical Oncology, Institute of Medical Science, University of Tokyo, Japan, and the Division of Hematology, Department of Internal Medicine, Keio University, Tokyo, Japan. The CD34 antigen serves as an important marker for primitive hematopoietic cells in therapeutic transplantation of hematopoietic stem cells (HSC) and gene therapy, but it has remained an open question as to whether or not most HSC express CD34. Using a competitive long-term reconstitution assay, the results of this study confirm developmental changes in CD34 expression on murine HSC. In fetuses and neonates, CD34 was expressed on Linc-Kit+ long-term repopulating HSC of bone marrow (BM), liver, and spleen. However, CD34 expression on HSC decreased with aging, and in mice older than 10 weeks, HSC were most enriched in the Linc-Kit+CD34 marrow cell fraction. A second transplantation was performed from primary recipients who were transplanted with neonatal Linc-Kit+ CD34high HSC marrow. Although donor-type HSC resided in CD34-expressing cell fraction in BM cells of the first recipients 4 weeks after the first transplantation, the stem cell activity had shifted to Linc-Kit+CD34 cells after 16 weeks, indicating that adult Linc-Kit+CD34 HSC are the progeny of neonatal CD34-expresssing HSC. Assays for colony-forming cells showed that hematopoietic progenitor cells, unlike HSC, continue to express CD34 throughout murine development. The present findings are important because the clinical application of HSC can be extended, in particular as related to CD34-enriched HSC and umbilical cord blood HSC. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Yokota, K. Oritani, S. Butz, K. Kokame, P. W. Kincade, T. Miyata, D. Vestweber, and Y. Kanakura The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice Blood, March 26, 2009; 113(13): 2914 - 2923. [Abstract] [Full Text] [PDF] L. E. Purton, S. Dworkin, G. H. Olsen, C. R. Walkley, S. A. Fabb, S. J. Collins, and P. Chambon RAR{gamma} is critical for maintaining a balance between hematopoietic stem cell self-renewal and differentiation J. Exp. Med., May 15, 2006; 203(5): 1283 - 1293. [Abstract] [Full Text] [PDF] F. Martelli, B. Ghinassi, B. Panetta, E. Alfani, V. Gatta, A. Pancrazzi, C. Bogani, A. M. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020