|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 February 2001, Vol. 97, No. 3, pp. 708-713
NEOPLASIA
Characterization of novel natural killer (NK)-cell and  
T-cell lines established from primary lesions of nasal T/NK-cell
lymphomas associated with the Epstein-Barr virus
Hiroshi Nagata,
Akiyoshi Konno,
Nobuhiro Kimura,
Yu Zhang,
Michiko Kimura,
Ayako Demachi,
Teruaki Sekine,
Kohtaro Yamamoto, and
Norio Shimizu
From the Department of Otorhinolaryngology, School of
Medicine, Chiba University, Chiba; First Department of Internal
Medicine, School of Medicine, Fukuoka University, Fukuoka; and
Department of Virology, Division of Virology and Immunology, Medical
Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
Studies on nasal T/natural killer (NK)-cell lymphoma have
been hampered by its tendency to cause necrosis. Thus, the
establishment of cell lines of this neoplasm would seem to be valuable.
This study attempted to establish cell lines from primary lesions of this tumor, and successfully obtained 2 novel Epstein-Barr virus (EBV)-positive cell lines, SNK-6 and SNT-8, by means of high-dose recombinant interleukin 2. Flow cytometry showed that SNK-6 had an
NK-cell phenotype,
CD3 CD4CD8CD19CD56+
T-cell receptor (TCR)  / TCR  / ,
whereas SNT-8 was
CD3+CD4CD8CD19CD56+
TCR / TCR /+. These were
consistent with immunophenotypes of their original tumors, and the cell
lines had monoclonal EBV clones identical to ones in their original
tumors. Thus, the cell lines developed from cells forming the primary
lesions. Genotypic analysis showed that SNK-6 had unrearranged TCR and
immunoglobulin heavy-chain genes, supporting the conclusion that SNK-6
was of NK-cell lineage. On the other hand, SNT-8 had rearranged TCR
-, -, and -chain genes, and together with its phenotype, SNT-8
proved to be a T-cell line. This is the first report of the
establishment of cell lines from primary lesions of nasal T/NK cell
lymphomas, and the results demonstrated that there are at least 2 lineages, NK- and T-cell, in this neoplasm. Moreover, it has
been suggested that nasal T/NK cell lymphomas of these lineages may
belong to the same clinicopathologic entity because both types of cases shared common clinical and histopathologic features.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Moriai, M. Takahara, T. Ogino, T. Nagato, K. Kishibe, H. Ishii, A. Katayama, N. Shimizu, and Y. Harabuchi
Production of Interferon-{gamma}-Inducible Protein-10 and Its Role as an Autocrine Invasion Factor in Nasal Natural Killer/T-Cell Lymphoma Cells
Clin. Cancer Res.,
November 15, 2009;
15(22):
6771 - 6779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yamanaka, H. Tagawa, N. Takahashi, A. Watanabe, Y.-M. Guo, K. Iwamoto, J. Yamashita, H. Saitoh, Y. Kameoka, N. Shimizu, et al.
Aberrant overexpression of microRNAs activate AKT signaling via down-regulation of tumor suppressors in natural killer-cell lymphoma/leukemia
Blood,
October 8, 2009;
114(15):
3265 - 3275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Shen, W.-Y. Au, K.-Y. Wong, N. Shimizu, J. Tsuchiyama, Y.-L. Kwong, R. H. Liang, and G. Srivastava
Cell death by bortezomib-induced mitotic catastrophe in natural killer lymphoma cells
Mol. Cancer Ther.,
December 1, 2008;
7(12):
3807 - 3815.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kobayashi, T. Nagato, M. Takahara, K. Sato, S. Kimura, N. Aoki, M. Azumi, M. Tateno, Y. Harabuchi, and E. Celis
Induction of EBV-Latent Membrane Protein 1-Specific MHC Class II-Restricted T-Cell Responses against Natural Killer Lymphoma Cells
Cancer Res.,
February 1, 2008;
68(3):
901 - 908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. L. Kis, M. Takahara, N. Nagy, G. Klein, and E. Klein
IL-10 can induce the expression of EBV-encoded latent membrane protein-1 (LMP-1) in the absence of EBNA-2 in B lymphocytes and in Burkitt lymphoma- and NK lymphoma-derived cell lines
Blood,
April 1, 2006;
107(7):
2928 - 2935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nagato, H. Kobayashi, K. Kishibe, M. Takahara, T. Ogino, H. Ishii, K. Oikawa, N. Aoki, K. Sato, S. Kimura, et al.
Expression of Interleukin-9 in Nasal Natural Killer/T-Cell Lymphoma Cell Lines and Patients
Clin. Cancer Res.,
December 1, 2005;
11(23):
8250 - 8257.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Oyoshi, H. Nagata, N. Kimura, Y. Zhang, A. Demachi, T. Hara, H. Kanegane, Y. Matsuo, T. Yamaguchi, T. Morio, et al.
Preferential Expansion of V{gamma}9-J{gamma}P/V{delta}2-J{delta}3 {gamma}{delta} T Cells in Nasal T-Cell Lymphoma and Chronic Active Epstein-Barr Virus Infection
Am. J. Pathol.,
May 1, 2003;
162(5):
1629 - 1638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hayashi, Z. Jin, S. Onoda, H. Joko, N. Teramoto, N. Ohara, W. Oda, T. Tanaka, Y.-X. Liu, T. R. Koirala, et al.
Rabbit Model for Human EBV-Associated Hemophagocytic Syndrome (HPS): Sequential Autopsy Analysis and Characterization of IL-2-Dependent Cell Lines Established from Herpesvirus Papio-Induced Fatal Rabbit Lymphoproliferative Diseases with HPS
Am. J. Pathol.,
May 1, 2003;
162(5):
1721 - 1736.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-W. Lin, W.-H. Lee, C.-L. Chang, J.-Y. Yang, and S.-M. Hsu
Restricted Killer Cell Immunoglobulin-Like Receptor Repertoire without T-Cell Receptor {gamma} Rearrangement Supports a True Natural Killer-Cell Lineage in a Subset of Sinonasal Lymphomas
Am. J. Pathol.,
November 1, 2001;
159(5):
1671 - 1679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
