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Blood, 15 February 2001, Vol. 97, No. 4, pp. 1016-1022
IMMUNOBIOLOGY
Selective down-regulation of high-affinity IgE receptor (Fc RI)
 -chain messenger RNA among transcriptome in cord
blood-derived versus adult peripheral blood-derived cultured human
mast cells
Makoto Iida,
Kenji Matsumoto,
Hisashi Tomita,
Toshiharu Nakajima,
Akira Akasawa,
Noriko Yuyama Ohtani,
Ning
Lu Yoshida,
Keiko Matsui,
Akiko Nakada,
Yuji Sugita,
Yuji Shimizu,
Shunichi Wakahara,
Toru Nakao,
Yasuyuki Fujii,
Chisei Ra, and
Hirohisa Saito
From the Departments of Allergy and Immunology,
National Children's Medical Research Center, Tokyo; Genox Research,
Kanagawa; Department of Medicine, Gunma University School of Medicine,
Gunma; Molecular and Cellular Biology Group, Medical Research
Laboratories, and the Ethical Business Strategy Division, Taisho
Pharmaceutical, Saitama; and Allergy Research Center, Juntendo
University School of Medicine, Tokyo, Japan.
Substantial numbers of human mast cells (MCs) were generated from
umbilical cord blood (CB) and from adult peripheral blood (PB). A
single CB progenitor produced 15 436 MCs, whereas a single PB
progenitor produced 807 MCs on average. However, PB-derived MCs were
far more active than CB-derived MCs in terms of high-affinity IgE
receptor (Fc RI)-mediated reactions. One million sensitized PB-derived MCs released 3.6 µg histamine, 215 pg IL-5, and 14 ng
granulocyte macrophage-colony-stimulating factor (GM-CSF), whereas
106 sensitized CB-derived MCs released only 0.8 µg
histamine, 31 pg IL-5, and 0.58 ng GM-CSF on anti-IgE challenge.
However, ionophore A23 187 released similar levels of histamine from
the 2 MC types. PB-derived MCs highly expressed surface FcRI  chain, and CB-derived MCs almost lacked it in the absence of IgE.
PB-derived MCs expressed approximately 5 times higher levels of
messenger RNA (mRNA) for FcRI chain than CB-derived MCs, but
mRNAs for  and  chains of the receptors were equally expressed.
Among the approximately 5600 kinds of full-length human genes examined
by using the high-density oligonucleotide probe-array system,
FcRI was ranked the fifth most increased transcript in
PB-derived MCs. The 4 other increased transcripts were unrelated to MC
function. These results suggest that IgE-mediated reactions may be
restricted during early infancy through the selective inhibition of
FcRI transcription, which is probably committed at progenitor
stages and is, at least in part, cytokine-insensitive.
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