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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Canonne-Hergaux, F. Articles by Gros, P. Search for Related Content PubMed PubMed Citation Articles by Canonne-Hergaux, F. Articles by Gros, P. Related Collections Red Cells Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 February 2001, Vol. 97, No. 4, pp. 1138-1140 BRIEF REPORT Expression of the DMT1 (NRAMP2/DCT1) iron transporter in mice with genetic iron overload disorders François Canonne-Hergaux, Joanne E. Levy, Mark D. Fleming, Lynne K. Montross, Nancy C. Andrews, and Philippe Gros From the Department of Biochemistry, McGill University, Montreal, Canada; Division of Hematology/Oncology, Children's Hospital; Howard Hughes Medical Institute; Division of Hematology, Brigham and Women's Hospital; Department of Pathology, Children's Hospital; and Department of Pediatrics, Harvard Medical School, Boston, MA. Iron overload is highly prevalent, but its molecular pathogenesis is poorly understood. Recently, DMT1 was shown to be a major apical iron transporter in absorptive cells of the duodenum. In vivo, it is the only transporter known to be important for the uptake of dietary non-heme iron from the gut lumen. The expression and subcellular localization of DMT1 protein in 3 mouse models of iron overload were examined: hypotransferrinemic (Trfhpx) mice, Hfe knockout mice, and B2m knockout mice. Interestingly, in Trfhpx homozygotes, DMT1 expression was strongly induced in the villus brush border when compared to control animals. This suggests that DMT1 expression is increased in response to iron deficiency in the erythron, even in the setting of systemic iron overload. In contrast, no increase was seen in DMT1 expression in animals with iron overload resembling human hemochromatosis. Therefore, it does not appear that changes in DMT1 levels are primarily responsible for iron loading in hemochromatosis. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W.S. Chong, P.C. Kwan, L.Y. Chan, P.Y. Chiu, T.K. Cheung, and T.K. Lau Expression of divalent metal transporter 1 (DMT1) isoforms in first trimester human placenta and embryonic tissues Hum. Reprod., December 1, 2005; 20(12): 3532 - 3538. [Abstract] [Full Text] [PDF] T Kelleher, E Ryan, S Barrett, M Sweeney, V Byrnes, C O'Keane, and J Crowe Increased DMT1 but not IREG1 or HFE mRNA following iron depletion therapy in hereditary haemochromatosis Gut, August 1, 2004; 53(8): 1174 - 1179. [Abstract] [Full Text] [PDF] N. Touret, W. Furuya, J. Forbes, P. Gros, and S. Grinstein Dynamic Traffic through the Recycling Compartment Couples the Metal Transporter Nramp2 (DMT1) with the Transferrin Receptor J. Biol. Chem., July 3, 2003; 278(28): 25548 - 25557. [Abstract] [Full Text] [PDF] K A Stuart, G J Anderson, D M Frazer, L W Powell, M McCullen, L M Fletcher, and D H G Crawford Duodenal expression of iron transport molecules in untreated haemochromatosis subjects Gut, July 1, 2003; 52(7): 953 - 959. [Abstract] [Full Text] A. J. Ghio, X. Wang, R. Silbajoris, M. D. Garrick, C. A. Piantadosi, and F. 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[Abstract] [Full Text] [PDF] F Dupic, S Fruchon, M Bensaid, O Loreal, P Brissot, N Borot, M P Roth, and H Coppin Duodenal mRNA expression of iron related genes in response to iron loading and iron deficiency in four strains of mice Gut, November 1, 2002; 51(5): 648 - 653. [Abstract] [Full Text] [PDF] N. Hubert and M. W. Hentze Previously uncharacterized isoforms of divalent metal transporter (DMT)-1: Implications for regulation and cellular function PNAS, September 17, 2002; 99(19): 12345 - 12350. [Abstract] [Full Text] [PDF] T. Moos, D. Trinder, and E. H. Morgan Effect of iron status on DMT1 expression in duodenal enterocytes from beta 2-microglobulin knockout mice Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G687 - G694. [Abstract] [Full Text] [PDF] D Trinder, C Fox, G Vautier, and J K Olynyk Molecular pathogenesis of iron overload Gut, August 1, 2002; 51(2): 290 - 295. [Abstract] [Full Text] [PDF] D. Trinder, J. K. Olynyk, W. S. Sly, and E. H. Morgan Iron uptake from plasma transferrin by the duodenum is impaired in the Hfe knockout mouse PNAS, April 8, 2002; (2002) 82112299. [Abstract] [Full Text] [PDF] A. Rolfs, H. L. Bonkovsky, J. G. Kohlroser, K. McNeal, A. Sharma, U. V. Berger, and M. A. Hediger Intestinal expression of genes involved in iron absorption in humans Am J Physiol Gastrointest Liver Physiol, April 1, 2002; 282(4): G598 - G607. [Abstract] [Full Text] [PDF] F. Canonne-Hergaux, A.-S. Zhang, P. Ponka, and P. Gros Characterization of the iron transporter DMT1 (NRAMP2/DCT1) in red blood cells of normal and anemic mk/mk mice Blood, December 15, 2001; 98(13): 3823 - 3830. [Abstract] [Full Text] [PDF] G. Nicolas, M. Bennoun, I. Devaux, C. Beaumont, B. Grandchamp, A. Kahn, and S. Vaulont Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice PNAS, July 5, 2001; (2001) 151179498. [Abstract] [Full Text] [PDF] M. D. Knutson, J. E. Levy, N. C. Andrews, and M. Wessling-Resnick Expression of Stimulator of Fe Transport Is Not Enhanced in Hfe Knockout Mice J. Nutr., May 1, 2001; 131(5): 1459 - 1464. [Abstract] [Full Text] D. Trinder, J. K. Olynyk, W. S. Sly, and E. H. Morgan Iron uptake from plasma transferrin by the duodenum is impaired in the Hfe knockout mouse PNAS, April 16, 2002; 99(8): 5622 - 5626. [Abstract] [Full Text] [PDF] G. Nicolas, M. Bennoun, I. Devaux, C. Beaumont, B. Grandchamp, A. Kahn, and S. Vaulont From the Cover: Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice PNAS, July 17, 2001; 98(15): 8780 - 8785. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020