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Blood, 15 February 2001, Vol. 97, No. 4, pp. 850-857
CHEMOKINES
Expression of CCR9  -chemokine receptor is modulated in
thymocyte differentiation and is selectively maintained in
CD8+ T cells from secondary lymphoid organs
Laura Carramolino,
Ángel Zaballos,
Leonor Kremer,
Ricardo Villares,
Pilar Martín,
Carlos Ardavín,
Carlos Martínez-A, and
Gabriel Márquez
From the Departamento de Inmunología y
Oncología, Centro Nacional de Biotecnología,
Universidad Autónoma de Madrid, Cantoblanco, and the Departamento
de Biología Celular, Facultad de Biología, Universidad
Complutense, Madrid, Spain.
Chemokines appear to have an important role in the seeding of
lymphoid progenitors in the thymus, the regulation of the coordinated movements of the maturing T cells within this organ, and the egress of
the resulting naive T cells to secondary lymphoid organs. CCR9, the
specific receptor for the  -chemokine TECK/CCL25, is selectively expressed in thymus, lymph node, and spleen. Using a specific anti-CCR9
polyclonal antibody, K629, and a semiquantitative reverse transcriptase-polymerase chain reaction procedure, a detailed study of CCR9 expression in the thymus and secondary lymphoid organs
was performed. The results show that
CD4+CD8+ double-positive thymocytes
have the highest CCR9 expression in thymus. Single-positive
CD8+ thymocytes continue to express this receptor after
abandoning the thymus as mature naive T cells, as suggested by the
existence of a CD8+CD69lowCD62Lhigh
CCR9+ cell subset. Consistent with this, CD8+
lymphocytes from lymph nodes, spleen, and Peyer patches express a
functional CCR9, as its expression correlates with migration in
response to CCL25. Conversely, CD4+ thymocytes lose CCR9
before abandoning the thymus, and CD4+ T cells from
secondary lymphoid organs also lack CCR9 expression. Analysis of CCR9
expression in thymocytes from mice of different ages showed that CCR9
levels are affected by age, as this receptor is more abundant, and its
response to CCL25 is more potent in newborn animals. Collectively,
these results suggest that CCR9 has a role in thymocyte development
throughout murine life, with clear differences between the
CD4+ and CD8+ lineages.
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