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Related Collections Transplantation Immunotherapy Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 March 2001, Vol. 97, No. 5, pp. 1232-1240 CLINICAL OBSERVATIONS, INTERVENTIONS, AND THERAPEUTIC TRIALS Direct visualization of cytomegalovirus-specific T-cell reconstitution after allogeneic stem cell transplantation Kate Cwynarski, Jenni Ainsworth, Mark Cobbold, Simon Wagner, Prem Mahendra, Jane Apperley, John Goldman, Charles Craddock, and Paul A. H. Moss From the CRC Institute for Cancer Studies, University of Birmingham, Edgbaston, United Kingdom; the Department of Hematology, University Hospital, Birmingham; and the Department of Hematology, Hammersmith Hospital, ICSM, London, United Kingdom. Cytomegalovirus (CMV) remains an important cause of morbidity and mortality after allogeneic stem cell transplantation (SCT), but cytotoxic T lymphocytes (CTL) may play a critical role in controlling CMV reactivation. Fluorescent HLA-peptide tetramers containing immunodominant peptides from CMV were used to prospectively monitor the recovery of CMV CTL in recipients of allogeneic transplants from siblings (n = 13) or unrelated donors (n = 11). In patients given allografts from a sibling when both the patient and donor were seropositive for CMV before SCT, recovery of CMV-specific CTL was rapid and reached up to 21% of all CD8+ T cells. Early reconstitution of CMV-specific immunity was not observed if either the donor or recipient was seronegative for CMV. In recipients of transplants from volunteer unrelated donors, recovery of CMV-specific CTL was delayed in comparison to that in recipients of transplants from siblings and no CTL were observed within the first 100 days after SCT. CTL numbers were increased after episodes of CMV reactivation but were suppressed by prednisolone therapy. Recovery of CMV-specific CTL to levels greater than 10 × 106/L was associated with protection from CMV disease. It was concluded that use of HLA-peptide tetramers to quantify CMV CTL is valuable for studying T-cell responses after allogeneic SCT. It should allow prediction of CMV reactivation in individual patients and assist in the development of adoptive T-cell immunotherapy. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. Sacre, S. Nguyen, C. Deback, G. Carcelain, J.-P. Vernant, V. Leblond, B. Autran, and N. Dhedin Expansion of Human Cytomegalovirus (HCMV) Immediate-Early 1-Specific CD8+ T Cells and Control of HCMV Replication after Allogeneic Stem Cell Transplantation J. Virol., October 15, 2008; 82(20): 10143 - 10152. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020