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Blood, 1 April 2001, Vol. 97, No. 7, pp. 2023-2030
HEMATOPOIESIS
Different expression of CD41 on human lymphoid and myeloid
progenitors from adults and neonates
Najet Debili,
Catherine Robin,
Valérie Schiavon,
Rémi Letestu,
Françoise Pflumio,
Maria-Teresa Mitjavila-Garcia,
Laure Coulombel, and
William Vainchenker
From Institut National de la Santé et de la
Recherche Médicale (INSERM) U 362, Institut Gustave Roussy,
Cedex, France.
The glycoprotein (Gp) IIb/IIIa integrin, also called CD41, is the
platelet receptor for fibrinogen and several other extracellular matrix
molecules. Recent evidence suggests that its expression is much wider
in the hematopoietic system than was previously thought. To investigate
the precise expression of the CD41 antigen during megakaryocyte (MK)
differentiation, CD34+ cells from cord blood and mobilized
blood cells from adults were grown for 6 days in the presence of stem
cell factor and thrombopoietin. Two different pathways of
differentiation were observed: one in the adult and one in the neonate
cells. In the neonate samples, early MK differentiation proceeded from
CD34+CD41 through a
CD34CD41+CD42 stage of
differentiation to more mature cells. In contrast, in the adult
samples, CD41 and CD42 were co-expressed on a CD34+ cell.
The rare CD34+CD41+CD42 cell
subset in neonates was not committed to MK differentiation but
contained cells with all myeloid and lymphoid potentialities along with
long-term culture initiating cells (LTC-ICs) and nonobese diabetic/severe combined immune-deficient repopulating cells. In the
adult samples, the CD34+CD41+CD42
subset was enriched in MK progenitors, but also contained erythroid progenitors, rare myeloid progenitors, and some LTC-ICs. All together, these results demonstrate that the CD41 antigen is expressed at a low
level on primitive hematopoietic cells with a myeloid and lymphoid
potential and that its expression is ontogenically regulated, leading
to marked differences in the surface antigenic properties of
differentiating megakaryocytic cells from neonates and adults.
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