|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 April 2001, Vol. 97, No. 8, pp. 2342-2350
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Expression and colocalization of cytokeratin 1 and
urokinase plasminogen activator receptor on endothelial cells
Fakhri Mahdi,
Zia Shariat-Madar,
Robert F. Todd III,
Carlos D. Figueroa, and
Alvin H. Schmaier
From the Hematology and Oncology Division, Department
of Internal Medicine and Pathology, University of Michigan, Ann Arbor,
MI, and Institute of Histology and Pathology, Austral University,
Valdivia, Chile.
The cellular localization of human cytokeratin 1 (CK1), urokinase
plasminogen activator receptor (uPAR), and gC1qR, high-molecular-weight kininogen (HK)-binding proteins on endothelial cells, was determined. CK1 was found on the external membrane of nonpermeabilized endothelial cells by immunoperoxidase staining, immunofluorescence, and
transmission electron microscopy using immunogold. Human umbilical vein
endothelial cells (HUVECs) had 7.2 ± 0.2 × 104
specific CK1 membrane sites/cell by
125I-F(ab')2 anti-CK1 antibody binding. Flow
cytometry studies confirmed the presence of CK1, uPAR, and gC1qR on
HUVECs. On laser scanning confocal microscopy and transmission
electron microscopy, CK1 and uPAR, but not gC1qR, colocalized on the
cell surface of HUVECs. The HUVEC surface distribution of these
proteins was distinctly different from that for von Willebrand factor.
In competitive inhibition experiments, anti-CK1, anti-uPAR, or
anti-gC1qR blocked both biotin-HK binding and prekallikrein (PK)
activation on HUVECs with an inhibitory concentration of 50%
(IC50) of 300 to 350 nM, 50 to 60 nM, or 35 to 100 nM,
respectively. Also, antibodies to uPAR and gC1qR each inhibited 86% of
kallikrein-mediated, 2-chain urokinase plasminogen activation, whereas
antibodies to CK1 only inhibited 24% of plasminogen activation. On
HUVECs, CK1 and uPAR, but not gC1qR, colocalized to be a
multiprotein receptor complex for HK binding, PK activation, and
2-chain urokinase plasminogen activation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. M. Astern, W. F. Pendergraft III, R. J. Falk, J. C. Jennette, A. H. Schmaier, F. Mahdi, and G. A. Preston
Myeloperoxidase Interacts with Endothelial Cell-Surface Cytokeratin 1 and Modulates Bradykinin Production by the Plasma Kallikrein-Kinin System
Am. J. Pathol.,
July 1, 2007;
171(1):
349 - 360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Gondi, N. Kandhukuri, S. Kondraganti, M. Gujrati, W. C. Olivero, D. H. Dinh, and J. S. Rao
RNA interference-mediated simultaneous down-regulation of urokinase-type plasminogen activator receptor and cathepsin B induces caspase-8-mediated apoptosis in SNB19 human glioma cells
Mol. Cancer Ther.,
December 1, 2006;
5(12):
3197 - 3208.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Khan, H. N. Bradford, I. Isordia-Salas, Y. Liu, Y. Wu, R. G. Espinola, B. Ghebrehiwet, and R. W. Colman
High-Molecular-Weight Kininogen Fragments Stimulate the Secretion of Cytokines and Chemokines Through uPAR, Mac-1, and gC1qR in Monocytes
Arterioscler. Thromb. Vasc. Biol.,
October 1, 2006;
26(10):
2260 - 2266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Tang, C. L. Yu, S. R. Williams, E. Springman, D. Jeffery, P. A. Sprengeler, A. Estevez, J. Sampang, W. Shrader, J. Spencer, et al.
Expression, Crystallization, and Three-dimensional Structure of the Catalytic Domain of Human Plasma Kallikrein
J. Biol. Chem.,
December 9, 2005;
280(49):
41077 - 41089.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Shariat-Madar, E. Rahimy, F. Mahdi, and A. H. Schmaier
Overexpression of prolylcarboxypeptidase enhances plasma prekallikrein activation on Chinese hamster ovary cells
Am J Physiol Heart Circ Physiol,
December 1, 2005;
289(6):
H2697 - H2703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Miceli, A. Tropea, F. Minici, M. Orlando, G. Lamanna, M. F. Gangale, B. Panetta, F. Tiberi, S. Vaccari, R. Canipari, et al.
Effects of Insulin-Like Growth Factor I and II on Prostaglandin Synthesis and Plasminogen Activator Activity in Human Umbilical Vein Endothelial Cells
J. Clin. Endocrinol. Metab.,
January 1, 2005;
90(1):
372 - 378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Shariat-Madar, F. Mahdi, and A. H. Schmaier
Recombinant prolylcarboxypeptidase activates plasma prekallikrein
Blood,
June 15, 2004;
103(12):
4554 - 4561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Cao, Y.-L. Guo, and R. W. Colman
Urokinase-Type Plasminogen Activator Receptor Is Involved in Mediating the Apoptotic Effect of Cleaved High Molecular Weight Kininogen in Human Endothelial Cells
Circ. Res.,
May 14, 2004;
94(9):
1227 - 1234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mahdi, Z. Shariat-Madar, A. Kuo, M. Carinato, D. B. Cines, and A. H. Schmaier
Mapping the Interaction between High Molecular Mass Kininogen and the Urokinase Plasminogen Activator Receptor
J. Biol. Chem.,
April 16, 2004;
279(16):
16621 - 16628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mahdi, Z. Shariat-Madar, and A. H. Schmaier
The Relative Priority of Prekallikrein and Factors XI/XIa Assembly on Cultured Endothelial Cells
J. Biol. Chem.,
November 7, 2003;
278(45):
43983 - 43990.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. H. Schmaier
The kallikrein-kinin and the renin-angiotensin systems have a multilayered interaction
Am J Physiol Regulatory Integrative Comp Physiol,
July 1, 2003;
285(1):
R1 - R13.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Krijanovski, V. Proulle, F. Mahdi, M. Dreyfus, W. Muller-Esterl, and A. H. Schmaier
Characterization of molecular defects of Fitzgerald trait and another novel high-molecular-weight kininogen-deficient patient: insights into structural requirements for kininogen expression
Blood,
June 1, 2003;
101(11):
4430 - 4436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. R. Baird and P. N. Walsh
Factor XI, but Not Prekallikrein, Blocks High Molecular Weight Kininogen Binding to Human Umbilical Vein Endothelial Cells
J. Biol. Chem.,
May 30, 2003;
278(23):
20618 - 20623.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. J. Laudes, J. C. Chu, M. Huber-Lang, R.-F. Guo, N. C. Riedemann, J. V. Sarma, F. Mahdi, H. S. Murphy, C. Speyer, K. T. Lu, et al.
Expression and Function of C5a Receptor in Mouse Microvascular Endothelial Cells
J. Immunol.,
November 15, 2002;
169(10):
5962 - 5970.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. R. Baird and P. N. Walsh
The Interaction of Factor XIa with Activated Platelets but Not Endothelial Cells Promotes the Activation of Factor IX in the Consolidation Phase of Blood Coagulation
J. Biol. Chem.,
October 4, 2002;
277(41):
38462 - 38467.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mahdi, Z. S. Madar, C. D. Figueroa, and A. H. Schmaier
Factor XII interacts with the multiprotein assembly of urokinase plasminogen activator receptor, gC1qR, and cytokeratin 1 on endothelial cell membranes
Blood,
May 15, 2002;
99(10):
3585 - 3596.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Shariat-Madar, F. Mahdi, and A. H. Schmaier
Identification and Characterization of Prolylcarboxypeptidase as an Endothelial Cell Prekallikrein Activator
J. Biol. Chem.,
May 10, 2002;
277(20):
17962 - 17969.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Feng, M. G. Tonnesen, E. I. B. Peerschke, and B. Ghebrehiwet
Cooperation of C1q Receptors and Integrins in C1q-Mediated Endothelial Cell Adhesion and Spreading
J. Immunol.,
March 1, 2002;
168(5):
2441 - 2448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. C. A. Lima, P. C. Almeida, I. L. S. Tersariol, V. Schmitz, A. H. Schmaier, L. Juliano, I. Y. Hirata, W. Muller-Esterl, J. R. Chagas, and J. Scharfstein
Heparan Sulfate Modulates Kinin Release by Trypanosoma cruzi through the Activity of Cruzipain
J. Biol. Chem.,
February 15, 2002;
277(8):
5875 - 5881.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. D. Collard, M. C. Montalto, W. R. Reenstra, J. A. Buras, and G. L. Stahl
Endothelial Oxidative Stress Activates the Lectin Complement Pathway : Role of Cytokeratin 1
Am. J. Pathol.,
September 1, 2001;
159(3):
1045 - 1054.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
