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Blood, 1 May 2001, Vol. 97, No. 9, pp. 2618-2624
HEMATOPOIESIS
Reciprocal expression of CD38 and CD34 by adult murine
hematopoietic stem cells
Fumihito Tajima,
Takao Deguchi,
Joseph H. Laver,
Haiqun Zeng, and
Makio Ogawa
From the Departments of Medicine and Pediatrics,
Medical University of South Carolina and Department of Veterans Affairs
Medical Center, Charleston, SC.
The effects of activation of adult murine stem cells on their
expression of CD38 were studied using a murine transplantation model.
First, the published finding that the majority of long-term engrafting
cells from normal adult steady-state marrow are CD38+ was
confirmed. Next, it was determined that the majority of stem cells
activated in vivo by injection of 5-fluorouracil (5-FU) or mobilized by
granulocyte colony-stimulating factor are CD38 . Stem
cells that were activated in culture with interleukin-11 and steel
factor were also CD38. Previous studies have shown that
expression of CD34 by adult stem cells is also modulated by in vivo or
in vitro activation. To determine whether there is reciprocal
expression of CD38 and CD34, 4 populations of post-5-FU marrow cells
were analyzed. The majority of the stem cells were in the
CD38CD34+ fraction. However, secondary
transplantation experiments indicated that when the bone marrow reaches
steady state, the majority of the stem cells become
CD38+CD34. In addition, the minority
populations of CD34+ stem cells that occur in steady-state
bone marrow are CD38. This reversible and reciprocal
expression of CD38 and CD34 by murine stem cells may have implications
for the phenotypes of human stem cells.
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