SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kouro, T. Articles by Kincade, P. W. Search for Related Content PubMed PubMed Citation Articles by Kouro, T. Articles by Kincade, P. W. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 May 2001, Vol. 97, No. 9, pp. 2708-2715 IMMUNOBIOLOGY Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators Taku Kouro, Kay L. Medina, Kenji Oritani, and Paul W. Kincade From the Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, OK; and the Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Japan. Recently, a collection of surface markers was exploited to isolate viable Lin TdT+ cells from murine bone marrow. These early pro-B cells were enriched for B-lineage lymphocyte precursor activity measured by short-term culture and had little responsiveness to myeloid growth factors. Early precursors can be propagated with remarkably high cloning frequencies in stromal cell-free, serum-free cultures, permitting this analysis of direct regulatory factors. Expression of the interleukin-7 receptor (IL-7R) chain marks functional precursors and IL-7 is necessary for progression beyond the CD45RA+ CD19 stage. Efficient survival and differentiation were only observed when stem cell factor and Flt-3 ligand were also present. IL-7-responsive CD19+ precursors are estrogen resistant. However, B-lineage differentiation was selectively abrogated when highly purified Lin precursors were treated with hormone in the absence of stromal cells. In addition, early stages of B lymphopoiesis were arrested by limitin, a new interferon (IFN)-like cytokine as well as IFN-, IFN-, or transforming growth factor  (TGF-), but not by epidermal growth factor (EGF). Lin TdT+ early pro-B cells are shown here to be CD27+ AA4.1+/Ki-67+ Ly-6C Ly-6A/Sca-1Lo/Thy-1CD43+ CD4+/CD16/32Lo/CD44Hi and similar in some respects to the "common lymphoid progenitors" (CLP) identified by others. Although early pro-B cells have lost myeloid differentiation potential, transplantation experiments described here reveal that at least some can generate T lymphocytes. Of particular importance is the demonstration that a pivotal early stage of lymphopoiesis is directly sensitive to negative regulation by hormones and cytokines. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. A. Dudakov, G. L. Goldberg, J. J. Reiseger, A. P. Chidgey, and R. L. Boyd Withdrawal of Sex Steroids Reverses Age- and Chemotherapy-Related Defects in Bone Marrow Lymphopoiesis J. Immunol., May 15, 2009; 182(10): 6247 - 6260. [Abstract] [Full Text] [PDF] T. Yokota, K. Oritani, K. P. Garrett, T. Kouro, M. Nishida, I. Takahashi, M. Ichii, Y. Satoh, P. W. Kincade, and Y. Kanakura Soluble Frizzled-Related Protein 1 Is Estrogen Inducible in Bone Marrow Stromal Cells and Suppresses the Earliest Events in Lymphopoiesis J. Immunol., November 1, 2008; 181(9): 6061 - 6072. [Abstract] [Full Text] [PDF] M. E. Enos, S. A. Bancos, T. Bushnell, and I. N. Crispe E2F4 Modulates Differentiation and Gene Expression in Hematopoietic Progenitor Cells during Commitment to the Lymphoid Lineage J. Immunol., March 15, 2008; 180(6): 3699 - 3707. [Abstract] [Full Text] [PDF] X. Chen, B. L. Esplin, K. P. Garrett, R. S. Welner, C. F. Webb, and P. W. Kincade Retinoids Accelerate B Lineage Lymphoid Differentiation J. Immunol., January 1, 2008; 180(1): 138 - 145. [Abstract] [Full Text] [PDF] E. M. Six, D. Bonhomme, M. Monteiro, K. Beldjord, M. Jurkowska, C. Cordier-Garcia, A. Garrigue, L. Dal Cortivo, B. Rocha, A. Fischer, et al. A human postnatal lymphoid progenitor capable of circulating and seeding the thymus J. Exp. Med., December 24, 2007; 204(13): 3085 - 3093. [Abstract] [Full Text] [PDF] S. L. Kalis, S.-K. Zhai, P.-C. Yam, P. L. Witte, and K. L. Knight Suppression of B lymphopoiesis at a lymphoid progenitor stage in adult rabbits Int. Immunol., June 1, 2007; 19(6): 801 - 811. [Abstract] [Full Text] [PDF] G. L. Goldberg, O. Alpdogan, S. J. Muriglan, M. V. Hammett, M. K. Milton, J. M. Eng, V. M. Hubbard, A. Kochman, L. M. Willis, A. S. Greenberg, et al. Enhanced Immune Reconstitution by Sex Steroid Ablation following Allogeneic Hemopoietic Stem Cell Transplantation J. Immunol., June 1, 2007; 178(11): 7473 - 7484. [Abstract] [Full Text] [PDF] O. Umland, W. N. Mwangi, B. M. Anderson, J. C. Walker, and H. T. Petrie The Blood Contains Multiple Distinct Progenitor Populations with Clonogenic B and T Lineage Potential J. Immunol., April 1, 2007; 178(7): 4147 - 4152. [Abstract] [Full Text] [PDF] C. P. Schneider, M. G. Schwacha, and I. H. Chaudry Impact of sex and age on bone marrow immune responses in a murine model of trauma-hemorrhage J Appl Physiol, January 1, 2007; 102(1): 113 - 121. [Abstract] [Full Text] [PDF] A. L. Zoller and G. J. Kersh Estrogen Induces Thymic Atrophy by Eliminating Early Thymic Progenitors and Inhibiting Proliferation of beta-Selected Thymocytes. J. Immunol., June 15, 2006; 176(12): 7371 - 7378. [Abstract] [Full Text] [PDF] H. Igarashi, Y. Baba, Y. Nagai, E. Jimi, S. Ghosh, and P. W Kincade NF-{kappa}B is dispensable for normal lymphocyte development in bone marrow but required for protection of progenitors from TNF{alpha} Int. Immunol., May 1, 2006; 18(5): 653 - 659. [Abstract] [Full Text] [PDF] L. L. Rumfelt, Y. Zhou, B. M. Rowley, S. A. Shinton, and R. R. Hardy Lineage specification and plasticity in CD19- early B cell precursors J. Exp. Med., March 20, 2006; 203(3): 675 - 687. [Abstract] [Full Text] [PDF] M. R. Ryan, R. Shepherd, J. K. Leavey, Y. Gao, F. Grassi, F. J. Schnell, W.-P. Qian, G. J. Kersh, M. N. Weitzmann, and R. Pacifici An IL-7-dependent rebound in thymic T cell output contributes to the bone loss induced by estrogen deficiency PNAS, November 15, 2005; 102(46): 16735 - 16740. [Abstract] [Full Text] [PDF] K. M. Roundy, G. Spangrude, J. J. Weis, and J. H. Weis Partial rescue of B cells in microphthalmic osteopetrotic marrow by loss of response to type I IFNs Int. Immunol., November 1, 2005; 17(11): 1495 - 1503. [Abstract] [Full Text] [PDF] K. Maeda, Y. Baba, Y. Nagai, K. Miyazaki, A. Malykhin, K. Nakamura, P. W. Kincade, N. Sakaguchi, and K. M. Coggeshall IL-6 blocks a discrete early step in lymphopoiesis Blood, August 1, 2005; 106(3): 879 - 885. [Abstract] [Full Text] [PDF] H. Igarashi, K. L. Medina, T. Yokota, M. I. D. Rossi, N. Sakaguchi, P. C. Comp, and P. W. Kincade Early lymphoid progenitors in mouse and man are highly sensitive to glucocorticoids Int. Immunol., May 1, 2005; 17(5): 501 - 511. [Abstract] [Full Text] [PDF] K. Kikuchi, A. Y. Lai, C.-L. Hsu, and M. Kondo IL-7 receptor signaling is necessary for stage transition in adult B cell development through up-regulation of EBF J. Exp. Med., April 18, 2005; 201(8): 1197 - 1203. [Abstract] [Full Text] [PDF] G.-J. Shim, M. Warner, H.-J. Kim, S. Andersson, L. Liu, J. Ekman, O. Imamov, M. E. Jones, E. R. Simpson, and J.-A. Gustafsson Aromatase-deficient mice spontaneously develop a lymphoproliferative autoimmune disease resembling Sjogren's syndrome PNAS, August 24, 2004; 101(34): 12628 - 12633. [Abstract] [Full Text] [PDF] V. Paharkova-Vatchkova, R. Maldonado, and S. Kovats Estrogen Preferentially Promotes the Differentiation of CD11c+ CD11bintermediate Dendritic Cells from Bone Marrow Precursors J. Immunol., February 1, 2004; 172(3): 1426 - 1436. [Abstract] [Full Text] [PDF] M. J. Osborn, P. L. Ryan, N. Kirchhof, A. Panoskaltsis-Mortari, F. Mortari, and K.-S. R. S. Tudor Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis Blood, February 1, 2004; 103(3): 843 - 851. [Abstract] [Full Text] [PDF] K. Nakamura, T. Kouro, P. W. Kincade, A. Malykhin, K. Maeda, and K. M. Coggeshall Src Homology 2-containing 5-Inositol Phosphatase (SHIP) Suppresses an Early Stage of Lymphoid Cell Development through Elevated Interleukin-6 Production by Myeloid Cells in Bone Marrow J. Exp. Med., January 20, 2004; 199(2): 243 - 254. [Abstract] [Full Text] [PDF] T. Yokota, C. S. R. Meka, T. Kouro, K. L. Medina, H. Igarashi, M. Takahashi, K. Oritani, T. Funahashi, Y. Tomiyama, Y. Matsuzawa, et al. Adiponectin, a Fat Cell Product, Influences the Earliest Lymphocyte Precursors in Bone Marrow Cultures by Activation of the Cyclooxygenase-Prostaglandin Pathway in Stromal Cells J. Immunol., November 15, 2003; 171(10): 5091 - 5099. [Abstract] [Full Text] [PDF] G.-J. Shim, L. Wang, S. Andersson, N. Nagy, L. L. Kis, Q. Zhang, S. Makela, M. Warner, and J.-A. Gustafsson Disruption of the estrogen receptor {beta} gene in mice causes myeloproliferative disease resembling chronic myeloid leukemia with lymphoid blast crisis PNAS, May 27, 2003; 100(11): 6694 - 6699. [Abstract] [Full Text] [PDF] M. I. D. Rossi, T. Yokota, K. L. Medina, K. P. Garrett, P. C. Comp, A. H. Schipul Jr, and P. W. Kincade B lymphopoiesis is active throughout human life, but there are developmental age-related changes Blood, January 15, 2003; 101(2): 576 - 584. [Abstract] [Full Text] [PDF] J. A. Strauchen and L. K. Miller Lymphoid Progenitor Cells in Human Tonsils International Journal of Surgical Pathology, January 1, 2003; 11(1): 21 - 24. [Abstract] [PDF] K. Oritani, S. Hirota, T. Nakagawa, I. Takahashi, S.-i. Kawamoto, M. Yamada, N. Ishida, T. Kadoya, Y. Tomiyama, P. W. Kincade, et al. T lymphocytes constitutively produce an interferonlike cytokine limitin characterized as a heat- and acid-stable and heparin-binding glycoprotein Blood, January 1, 2003; 101(1): 178 - 185. [Abstract] [Full Text] [PDF] L. M. Sedger, S. Hou, S. R. Osvath, M. B. Glaccum, J. J. Peschon, N. van Rooijen, and L. Hyland Bone Marrow B Cell Apoptosis During In Vivo Influenza Virus Infection Requires TNF-{alpha} and Lymphotoxin-{alpha} J. Immunol., December 1, 2002; 169(11): 6193 - 6201. [Abstract] [Full Text] [PDF] Y. Hashimoto, E. Montecino-Rodriguez, H. Leathers, R. P. Stephan, and K. Dorshkind B-cell development in the thymus is limited by inhibitory signals from the thymic microenvironment Blood, November 15, 2002; 100(10): 3504 - 3511. [Abstract] [Full Text] [PDF] T. Kouro, V. Kumar, and P. W. Kincade Relationships between early B- and NK-lineage lymphocyte precursors in bone marrow Blood, November 15, 2002; 100(10): 3672 - 3680. [Abstract] [Full Text] [PDF] J. Pfeilschifter, R. Koditz, M. Pfohl, and H. Schatz Changes in Proinflammatory Cytokine Activity after Menopause Endocr. Rev., February 1, 2002; 23(1): 90 - 119. [Abstract] [Full Text] [PDF] H. Igarashi, T. Kouro, T. Yokota, P. C. Comp, and P. W. Kincade Age and stage dependency of estrogen receptor expression by lymphocyte precursors PNAS, December 18, 2001; 98(26): 15131 - 15136. [Abstract] [Full Text] [PDF] L.-S. Lu, J. Tung, N. Baumgarth, O. Herman, M. Gleimer, L. A. Herzenberg, and L. A. Herzenberg Identification of a germ-line pro-B cell subset that distinguishes the fetal/neonatal from the adult B cell development pathway PNAS, March 5, 2002; 99(5): 3007 - 3012. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020