|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 May 2001, Vol. 97, No. 9, pp. 2863-2871
RED CELLS
Repression of ferritin expression increases the labile iron pool,
oxidative stress, and short-term growth of human
erythroleukemia cells
Or Kakhlon,
Yosef Gruenbaum, and
Zvi Ioav Cabantchik
From the Departments of Biological Chemistry and of
Genetics, Institute of Life Sciences, Hebrew University, Jerusalem,
Israel.
The role of ferritin expression on the labile iron pool of cells
and its implications for the control of cell proliferation were
assessed. Antisense oligodeoxynucleotides were used as tools for
modulating the expression of heavy and light ferritin subunits of K562
cells. mRNA and protein levels of each subunit were markedly reduced by
2-day treatment with antisense probes against the respective subunit.
Although the combined action of antisense probes against both subunits
reduced their protein expression, antisense repression of one subunit
led to an increased protein expression of the other. Antisense
treatment led to a rise in the steady-state labile iron pool, a rise in
the production of reactive oxygen species after pro-oxidative
challenges and in protein oxidation, and the down-regulation of
transferrin receptors. When compared to the repression of individual subunits, co-repression of each subunit evoked a more than additive increase in the labile iron pool and the extent of protein oxidation. These treatments had no detectable effects on the long-term growth of
cells. However, repression of ferritin synthesis facilitated the
renewal of growth and the proliferation of cells pre-arrested at the
G1/S phase. Renewed cell growth was significantly less dependent on external iron supply when ferritin synthesis was repressed
and its degradation inhibited by lysosomal antiproteases. This study
provides experimental evidence that links the effect of ferritin
repression on growth stimulation to the expansion of the labile iron pool.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K.-H. Zhang, H.-Y. Tian, X. Gao, W.-W. Lei, Y. Hu, D.-M. Wang, X.-C. Pan, M.-L. Yu, G.-J. Xu, F.-K. Zhao, et al.
Ferritin Heavy Chain-Mediated Iron Homeostasis and Subsequent Increased Reactive Oxygen Species Production Are Essential for Epithelial-Mesenchymal Transition
Cancer Res.,
July 1, 2009;
69(13):
5340 - 5348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Wallander, K. B. Zumbrennen, E. S. Rodansky, S. J. Romney, and E. A. Leibold
Iron-independent Phosphorylation of Iron Regulatory Protein 2 Regulates Ferritin during the Cell Cycle
J. Biol. Chem.,
August 29, 2008;
283(35):
23589 - 23598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Nie, G. Chen, A. D. Sheftel, K. Pantopoulos, and P. Ponka
In vivo tumor growth is inhibited by cytosolic iron deprivation caused by the expression of mitochondrial ferritin
Blood,
October 1, 2006;
108(7):
2428 - 2434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Antosiewicz, A. Herman-Antosiewicz, S. W. Marynowski, and S. V. Singh
c-Jun NH2-Terminal Kinase Signaling Axis Regulates Diallyl Trisulfide-Induced Generation of Reactive Oxygen Species and Cell Cycle Arrest in Human Prostate Cancer Cells.
Cancer Res.,
May 15, 2006;
66(10):
5379 - 5386.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Tilton, L. G. Gerwick, J. D. Hendricks, C. S. Rosato, G. Corley-Smith, S. A. Givan, G. S. Bailey, C. J. Bayne, and D. E. Williams
Use of a Rainbow Trout Oligonucleotide Microarray to Determine Transcriptional Patterns in Aflatoxin B1-Induced Hepatocellular Carcinoma Compared to Adjacent Liver
Toxicol. Sci.,
December 1, 2005;
88(2):
319 - 330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Carlyon, D. Ryan, K. Archer, and E. Fikrig
Effects of Anaplasma phagocytophilum on Host Cell Ferritin mRNA and Protein Levels
Infect. Immun.,
November 1, 2005;
73(11):
7629 - 7636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Xie, N. Zhang, H. Zhou, J. Li, Q. Li, T. Zarubin, S.-C. Lin, and J. Han
Distinct Roles of Basal Steady-State and Induced H-Ferritin in Tumor Necrosis Factor-Induced Death in L929 Cells
Mol. Cell. Biol.,
August 1, 2005;
25(15):
6673 - 6681.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Cozzi, B. Corsi, S. Levi, P. Santambrogio, G. Biasiotto, and P. Arosio
Analysis of the biologic functions of H- and L-ferritins in HeLa cells by transfection with siRNAs and cDNAs: evidence for a proliferative role of L-ferritin
Blood,
March 15, 2004;
103(6):
2377 - 2383.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Petrat, S. Paluch, E. Dogruoz, P. Dorfler, M. Kirsch, H.-G. Korth, R. Sustmann, and H. de Groot
Reduction of Fe(III) Ions Complexed to Physiological Ligands by Lipoyl Dehydrogenase and Other Flavoenzymes in Vitro: IMPLICATIONS FOR AN ENZYMATIC REDUCTION OF Fe(III) IONS OF THE LABILE IRON POOL
J. Biol. Chem.,
November 21, 2003;
278(47):
46403 - 46413.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Pietsch, J. Y. Chan, F. M. Torti, and S. V. Torti
Nrf2 Mediates the Induction of Ferritin H in Response to Xenobiotics and Cancer Chemopreventive Dithiolethiones
J. Biol. Chem.,
January 17, 2003;
278(4):
2361 - 2369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Truty, R. Malpe, and M. C. Linder
Iron Prevents Ferritin Turnover in Hepatic Cells
J. Biol. Chem.,
December 21, 2001;
276(52):
48775 - 48780.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
