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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Langerak, A. W. Articles by van Dongen, J. J. M. Search for Related Content PubMed PubMed Citation Articles by Langerak, A. W. Articles by van Dongen, J. J. M. Related Collections Immunobiology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 July 2001, Vol. 98, No. 1, pp. 165-173 IMMUNOBIOLOGY Molecular and flow cytometric analysis of the V repertoire for clonality assessment in mature TCR T-cell proliferations Anton W. Langerak, René van den Beemd, Ingrid L. M. Wolvers-Tettero, Patrick P. C. Boor, Ellen G. van Lochem, Herbert Hooijkaas, and Jacques J. M. van Dongen From the Department of Immunology, University Hospital Rotterdam/Erasmus University Rotterdam, The Netherlands. Clonality assessment through Southern blot (SB) analysis of TCRB genes or polymerase chain reaction (PCR) analysis of TCRG genes is important for diagnosing suspect mature T-cell proliferations. Clonality assessment through reverse transcription (RT)-PCR analysis of V-C transcripts and flow cytometry with a V antibody panel covering more than 65% of V domains was validated using 28 SB-defined clonal T-cell receptor (TCR)+ T-ALL samples and T-cell lines. Next, the diagnostic applicability of the V RT-PCR and flow cytometric clonality assays was studied in 47 mature T-cell proliferations. Clonal V-C RT-PCR products were detected in all 47 samples, whereas single V domain usage was found in 31 (66%) of 47 patients. The suspect leukemic cell populations in the other 16 patients showed a complete lack of V monoclonal antibody reactivity that was confirmed by molecular data showing the usage of V gene segments not covered by the applied V monoclonal antibodies. Nevertheless, this could be considered indirect evidence for the "clonal" character of these cells. Remarkably, RT-PCR revealed an oligoclonal pattern in addition to dominant V-C products and single V domain expression in many T-LGL proliferations, providing further evidence for the hypothesis raised earlier that T-LGL derive from polyclonal and oligoclonal proliferations of antigen-activated cytotoxic T cells. It is concluded that molecular V analysis serves to assess clonality in suspect T-cell proliferations. However, the faster and cheaper V antibody studies can be used as a powerful screening method for the detection of single V domain expression, followed by molecular studies in patients with more than 20% single V domain expression or large suspect T-cell populations (more than 50%-60%) without V reactivity. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. R. Mohan, M. J. Clemente, M. Afable, H. N. Cazzolli, N. Bejanyan, M. W. Wlodarski, A. E. Lichtin, and J. P. Maciejewski Therapeutic implications of variable expression of CD52 on clonal cytotoxic T cells in CD8+ large granular lymphocyte leukemia Haematologica, October 1, 2009; 94(10): 1407 - 1414. [Abstract] [Full Text] [PDF] Z H Shah, S Harris, J L Smith, and E Hodges Monoclonality and oligoclonality of T cell receptor {beta} gene in angioimmunoblastic T cell lymphoma J. Clin. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020