SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lieschke, G. J. Articles by Layton, J. E. Search for Related Content PubMed PubMed Citation Articles by Lieschke, G. J. Articles by Layton, J. E. Related Collections Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 November 2001, Vol. 98, No. 10, pp. 3087-3096 PHAGOCYTES Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish Graham J. Lieschke, Andrew C. Oates, Meredith O. Crowhurst, Alister C. Ward, and Judith E. Layton From the Ludwig Institute for Cancer Research, The Royal Melbourne Hospital, Parkville, Victoria, Australia; and the Department of Molecular Biology, Princeton University, NJ. The zebrafish is a useful model organism for developmental and genetic studies. The morphology and function of zebrafish myeloid cells were characterized. Adult zebrafish contain 2 distinct granulocytes, a heterophil and a rarer eosinophil, both of which circulate and are generated in the kidney, the adult hematopoietic organ. Heterophils show strong histochemical myeloperoxidasic activity, although weaker peroxidase activity was observed under some conditions in eosinophils and erythrocytes. Embryonic zebrafish have circulating immature heterophils by 48 hours after fertilization (hpf). A zebrafish myeloperoxidase homologue (myeloid-specific peroxidase; mpx) was isolated. Phylogenetic analysis suggested it represented a gene ancestral to the mammalian myeloperoxidase gene family. It was expressed in adult granulocytes and in embryos from 18 hpf, first diffusely in the axial intermediate cell mass and then discretely in a dispersed cell population. Comparison of hemoglobinized cell distribution, mpx gene expression, and myeloperoxidase histochemistry in wild-type and mutant embryos confirmed that the latter reliably identified a population of myeloid cells. Studies in embryos after tail transection demonstrated that mpx- and peroxidase-expressing cells were mobile and localized to a site of inflammation, indicating functional capability of these embryonic granulocytes. Embryonic macrophages removed carbon particles from the circulation by phagocytosis. Collectively, these observations have demonstrated the early onset of zebrafish granulopoiesis, have proved that granulocytes circulate by 48 hpf, and have demonstrated the functional activity of embryonic granulocytes and macrophages. These observations will facilitate the application of this genetically tractable organism to the study of myelopoiesis. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Cvejic, C. Hall, M. Bak-Maier, M. V. Flores, P. Crosier, M. J. Redd, and P. Martin Analysis of WASp function during the wound inflammatory response - live-imaging studies in zebrafish larvae J. Cell Sci., October 1, 2008; 121(19): 3196 - 3206. [Abstract] [Full Text] [PDF] J. T. Dobson, J. Seibert, E. M. Teh, S. Da'as, R. B. Fraser, B. H. Paw, T.-J. Lin, and J. N. Berman Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determination Blood, October 1, 2008; 112(7): 2969 - 2972. [Abstract] [Full Text] [PDF] R. M. B. Costa, X. Soto, Y. Chen, A. M. Zorn, and E. Amaya spib is required for primitive myeloid development in Xenopus Blood, September 15, 2008; 112(6): 2287 - 2296. [Abstract] [Full Text] [PDF] S. Sumanas, G. Gomez, Y. Zhao, C. Park, K. Choi, and S. Lin Interplay among Etsrp/ER71, Scl, and Alk8 signaling controls endothelial and myeloid cell formation Blood, May 1, 2008; 111(9): 4500 - 4510. [Abstract] [Full Text] [PDF] J. B. Goldberg, R. E. W. Hancock, R. E. Parales, J. Loper, and P. Cornelis Pseudomonas 2007 J. Bacteriol., April 15, 2008; 190(8): 2649 - 2662. [Full Text] [PDF] D. Carradice and G. J. Lieschke Zebrafish in hematology: sushi or science? Blood, April 1, 2008; 111(7): 3331 - 3342. [Abstract] [Full Text] [PDF] J.-R. J. Yeh, K. M. Munson, Y. L. Chao, Q. P. Peterson, C. A. MacRae, and R. T. Peterson AML1-ETO reprograms hematopoietic cell fate by downregulating scl expression Development, January 15, 2008; 135(2): 401 - 410. [Abstract] [Full Text] [PDF] D. Le Guyader, M. J. Redd, E. Colucci-Guyon, E. Murayama, K. Kissa, V. Briolat, E. Mordelet, A. Zapata, H. Shinomiya, and P. Herbomel Origins and unconventional behavior of neutrophils in developing zebrafish Blood, January 1, 2008; 111(1): 132 - 141. [Abstract] [Full Text] [PDF] L. J. McReynolds, S. Gupta, M. E. Figueroa, M. C. Mullins, and T. Evans Smad1 and Smad5 differentially regulate embryonic hematopoiesis Blood, December 1, 2007; 110(12): 3881 - 3890. [Abstract] [Full Text] [PDF] J. R. Mathias, M. E. Dodd, K. B. Walters, J. Rhodes, J. P. Kanki, A. T. Look, and A. Huttenlocher Live imaging of chronic inflammation caused by mutation of zebrafish Hai1 J. Cell Sci., October 1, 2007; 120(19): 3372 - 3383. [Abstract] [Full Text] [PDF] T. J. Carney, S. von der Hardt, C. Sonntag, A. Amsterdam, J. Topczewski, N. Hopkins, and M. Hammerschmidt Inactivation of serine protease Matriptase1a by its inhibitor Hai1 is required for epithelial integrity of the zebrafish epidermis Development, October 1, 2007; 134(19): 3461 - 3471. [Abstract] [Full Text] [PDF] H. A. Phelps and M. N. Neely SalY of the Streptococcus pyogenes Lantibiotic Locus Is Required for Full Virulence and Intracellular Survival in Macrophages Infect. Immun., September 1, 2007; 75(9): 4541 - 4551. [Abstract] [Full Text] [PDF] A. L. Lumsden, T. L. Henshall, S. Dayan, M. T. Lardelli, and R. I. Richards Huntingtin-deficient zebrafish exhibit defects in iron utilization and development Hum. Mol. Genet., August 15, 2007; 16(16): 1905 - 1920. [Abstract] [Full Text] [PDF] S. B. Brown, C. S. Tucker, C. Ford, Y. Lee, D. R. Dunbar, and J. J. Mullins Class III antiarrhythmic methanesulfonanilides inhibit leukocyte recruitment in zebrafish J. Leukoc. Biol., July 1, 2007; 82(1): 79 - 84. [Abstract] [Full Text] [PDF] Y. Liu, L. Du, M. Osato, E. H. Teo, F. Qian, H. Jin, F. Zhen, J. Xu, L. Guo, H. Huang, et al. The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development Blood, July 1, 2007; 110(1): 99 - 106. [Abstract] [Full Text] [PDF] C. Grabher, A. Cliffe, K. Miura, J. Hayflick, R. Pepperkok, P. Rorth, and J. Wittbrodt Birth and life of tissue macrophages and their migration in embryogenesis and inflammation in medaka J. Leukoc. Biol., January 1, 2007; 81(1): 263 - 271. [Abstract] [Full Text] [PDF] G. J. Lieschke Fluorescent neutrophils throw the spotlight on inflammation Blood, December 15, 2006; 108(13): 3961 - 3962. [Full Text] [PDF] S. A. Renshaw, C. A. Loynes, D. M.I. Trushell, S. Elworthy, P. W. Ingham, and M. K.B. Whyte A transgenic zebrafish model of neutrophilic inflammation Blood, December 15, 2006; 108(13): 3976 - 3978. [Abstract] [Full Text] [PDF] J. R. Mathias, B. J. Perrin, T.-X. Liu, J. Kanki, A. T. Look, and A. Huttenlocher Resolution of inflammation by retrograde chemotaxis of neutrophils in transgenic zebrafish J. Leukoc. Biol., December 1, 2006; 80(6): 1281 - 1288. [Abstract] [Full Text] [PDF] A. M. van der Sar, O. W. Stockhammer, C. van der Laan, H. P. Spaink, W. Bitter, and A. H. Meijer MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model Infect. Immun., April 1, 2006; 74(4): 2436 - 2441. [Abstract] [Full Text] [PDF] M. A. Juarez, F. Su, S. Chun, M. J. Kiel, and S. E. Lyons Distinct Roles for SCL in Erythroid Specification and Maturation in Zebrafish J. Biol. Chem., December 16, 2005; 280(50): 41636 - 41644. [Abstract] [Full Text] [PDF] E. Chaves-Pozo, V. Mulero, J. Meseguer, and A. G. Ayala Professional phagocytic granulocytes of the bony fish gilthead seabream display functional adaptation to testicular microenvironment J. Leukoc. Biol., August 1, 2005; 78(2): 345 - 351. [Abstract] [Full Text] [PDF] D. M. Hentschel, K. M. Park, L. Cilenti, A. S. Zervos, I. Drummond, and J. V. Bonventre Acute renal failure in zebrafish: a novel system to study a complex disease Am J Physiol Renal Physiol, May 1, 2005; 288(5): F923 - F929. [Abstract] [Full Text] [PDF] J. F. Rawls, B. S. Samuel, and J. I. Gordon From The Cover: Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota PNAS, March 30, 2004; 101(13): 4596 - 4601. [Abstract] [Full Text] [PDF] A. Brenot, K. Y. King, B. Janowiak, O. Griffith, and M. G. Caparon Contribution of Glutathione Peroxidase to the Virulence of Streptococcus pyogenes Infect. Immun., January 1, 2004; 72(1): 408 - 413. [Abstract] [Full Text] [PDF] A. C. Ward, D. O. McPhee, M. M. Condron, S. Varma, S. H. Cody, S. M. N. Onnebo, B. H. Paw, L. I. Zon, and G. J. Lieschke The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish Blood, November 1, 2003; 102(9): 3238 - 3240. [Abstract] [Full Text] [PDF] J. M. Spitsbergen and M. L. Kent The State of the Art of the Zebrafish Model for Toxicology and Toxicologic Pathology Research--Advantages and Current Limitations Toxicol Pathol, January 1, 2003; 31(1_suppl): 62 - 87. [Abstract] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020