|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 December 2001, Vol. 98, No. 13, pp. 3717-3726
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Regulatory role of tetraspanin CD9 in tumor-endothelial cell
interaction during transendothelial invasion of melanoma cells
Natividad Longo,
María Yáñez-Mó,
María Mittelbrunn,
Gonzalo de la Rosa,
María-Luisa Muñoz,
Francisco Sánchez-Madrid, and
Paloma Sánchez-Mateos
From Servicio de Inmunología, Hospital General
Universitario Gregorio Marañón, and Servicio de
Inmunología, Hospital de la Princesa, Madrid, Spain.
Heterotypic interaction among tumor cells (TCs) and endothelial
cells (ECs) may play a critical role during the vascular dissemination of neoplastic cells and during pathologic angiogenesis in tumors. To
identify molecules involved in these processes, the distribution of
vascular junctional proteins was first studied by immunofluorescence at
sites of heterologous intercellular contact using TC-EC mosaic monolayers grown on 2-dimensional collagen. Several members of the
tetraspanin superfamily, including CD9, CD81, and CD151, were found to
localize at the TC-EC contact area. The localization of tetraspanins to
the TC-EC heterologous contact area was also observed during the active
transmigration of TCs across EC monolayers grown onto 3-dimensional
collagen matrices. Dynamic studies by time-lapse immunofluorescence
confocal microscopy showed an active redistribution of endothelial CD9
to points of melanoma insertion. Anti-CD9 monoclonal antibodies were
found to specifically inhibit the transendothelial migration of
melanoma cells; the inhibitory effect was likely caused by a
strengthening of CD9-mediated heterotypic interactions of TCs to the EC
monolayer. These data support a novel mechanism of tetraspanin-mediated
regulation of TC transcellular migration independent of TC motility and
growth during metastasis and a role for these molecules in the
formation of TC-EC mosaic monolayers during tumor angiogenesis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Estecha, L. Sanchez-Martin, A. Puig-Kroger, R. A. Bartolome, J. Teixido, R. Samaniego, and P. Sanchez-Mateos
Moesin orchestrates cortical polarity of melanoma tumour cells to initiate 3D invasion
J. Cell Sci.,
October 1, 2009;
122(19):
3492 - 3501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Zhang, J. Kotha, L. K. Jennings, and X. A. Zhang
Tetraspanins and vascular functions
Cardiovasc Res,
July 1, 2009;
83(1):
7 - 15.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gesierich, I. Berezovskiy, E. Ryschich, and M. Zoller
Systemic Induction of the Angiogenesis Switch by the Tetraspanin D6.1A/CO-029.
Cancer Res.,
July 15, 2006;
66(14):
7083 - 7094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Furuya, H. Kato, N. Nishimura, I. Ishiwata, H. Ikeda, R. Ito, T. Yoshiki, and H. Ishikura
Down-regulation of CD9 in Human Ovarian Carcinoma Cell Might Contribute to Peritoneal Dissemination: Morphologic Alteration and Reduced Expression of {beta}1 Integrin Subsets
Cancer Res.,
April 1, 2005;
65(7):
2617 - 2625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Barreiro, M. Yanez-Mo, M. Sala-Valdes, M. D. Gutierrez-Lopez, S. Ovalle, A. Higginbottom, P. N. Monk, C. Cabanas, and F. Sanchez-Madrid
Endothelial tetraspanin microdomains regulate leukocyte firm adhesion during extravasation
Blood,
April 1, 2005;
105(7):
2852 - 2861.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ang, M. Lijovic, L. K. Ashman, K. Kan, and A. G. Frauman
CD151 Protein Expression Predicts the Clinical Outcome of Low-Grade Primary Prostate Cancer Better than Histologic Grading: A New Prognostic Indicator?
Cancer Epidemiol. Biomarkers Prev.,
November 1, 2004;
13(11):
1717 - 1721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Mantegazza, M. M. Barrio, S. Moutel, L. Bover, M. Weck, P. Brossart, J.-L. Teillaud, and J. Mordoh
CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells
Blood,
August 15, 2004;
104(4):
1183 - 1190.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Sauer, J. Windisch, C. Kurzeder, V. Heilmann, R. Kreienberg, and H. Deissler
Progression of Cervical Carcinomas Is Associated with Down-Regulation of CD9 But Strong Local Re-expression at Sites of Transendothelial Invasion
Clin. Cancer Res.,
December 15, 2003;
9(17):
6426 - 6431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Gutierrez-Lopez, S. Ovalle, M. Yanez-Mo, N. Sanchez-Sanchez, E. Rubinstein, N. Olmo, M. A. Lizarbe, F. Sanchez-Madrid, and C. Cabanas
A Functionally Relevant Conformational Epitope on the CD9 Tetraspanin Depends on the Association with Activated beta 1 Integrin
J. Biol. Chem.,
January 3, 2003;
278(1):
208 - 218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
