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Blood, 1 August 2001, Vol. 98, No. 3, pp. 696-704
IMMUNOBIOLOGY
Characterization of thymus-seeding precursor cells from mouse
bone marrow
Shinichiro Mori,
Ken Shortman, and
Li Wu
From The Walter and Eliza Hall Institute of Medical
Research, Melbourne, Victoria, Australia.
The nature of the cells that seed the thymus of an irradiated
recipient after intravenous (IV) transfer of bone marrow (BM) cells was
investigated using 2 approaches. First, direct entry of a small number
of donor BM cells into the thymus was tracked using a Ly-5 marker.
Second, secondary IV transfer of the seeded thymus cells into a
secondary recipient was used as an assay for precursor activity. A
range of cell types was found to enter the recipient thymus initially,
including B-lineage cells and myeloid cells, but T precursors were
undetectable by flow cytometry over the first few days. Although all
cells initially entering the thymus proliferated, no sustained thymus
reconstitution was seen until day 4, when recognizable T-lineage
precursors began to appear. The secondary transfer assays revealed the
presence of lymphoid precursors in the recipient thymus, including T,
NKT, NK, and B precursor activity, with a notable early burst of
B-lineage generative capacity. There was no evidence of sustained
myeloid precursor or multipotent stem cell activity, even though these were seen if BM cells were injected directly into the recipient thymus
rather than introduced into the bloodstream. It is concluded that even
though many cell types may initially enter an irradiated thymus, the
thymus acts as a sieve, allowing lymphoid precursors, but not
multipotent stem cells, to seed the environmental niches that
permit selected precursor cell development and thymus reconstitution.
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