Blood, 15 August 2001, Vol. 98, No. 4, pp. 1054-1054
ERRATUM
In the article by Roy et al entitled "Asymmetrical
segregation of chromosomes with a normal metaphase/anaphase checkpoint in polyploid megakaryocytes," which appeared in the April 15, 2001, issue of Blood (Volume 97:2238-2247), Figure 7 should have appeared in color, as below.
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| Figure 7.
Studies of hCdh1 and cyclin B1
expression in megakaryocytes.
Immunofluorescence was performed on day 6 megakaryocyte culture as
described in Figure 5. (A) HCdh1 immunodetection in human
megakaryocytes. (i) Interphase polyploid megakaryocyte shows a nuclear
hCdh1 staining, using a polyclonal rabbit antibody followed by donkey
TRITC-labeled F(ab')2 fragments. DNA was counterstained with DAPI. (ii)
Double immunostaining of hCdh1 and tubulin with DNA painting. HCdh1 was
localized at the spindle poles during endomitosis. Bar, 10µm. (B)
Timing of cyclin B1 degradation in endomitosis. Cyclin B1 was
immunodetected by using a mouse antibody followed by incubation with
TRITC-conjugated antimouse F(ab')2 fragments. DNA was labeled with
DAPI. Three sequential phases of endomitosis were observed on a single
view: G2/M megakaryocyte in the middle with a strong nuclear staining,
prometaphase megakaryocyte in the lower-left corner with cyclin B1
localized at the spindle poles, and anaphase in the upper-right corner
in which cyclin B1 is undectectable. Bar, 10 µm.
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