|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2001, Vol. 98, No. 4, pp. 1122-1126
IMMUNOBIOLOGY
Peripheral blood stem cell allograft rejection mediated by
CD4+ T lymphocytes recognizing a single mismatch at
HLA-DP 1*0901
Katharina Fleischhauer,
Elisabetta Zino,
Benedetta Mazzi,
Elisabetta Sironi,
Paolo Servida,
Elisabetta Zappone,
Elena Benazzi, and
Claudio Bordignon
From the HLA Tissue Typing Laboratory and the BMT-Unit,
Istituto Scientifico H.S. Raffaele; and the San Raffaele Telethon
Institute for Gene Therapy (HSR-TIGET), Milan, Italy.
Little is known about the molecular characteristics of alloantigens
recognized by alloreactive T cells mediating hematologic stem cell
graft rejection. In particular, it has never been shown that such
alloantigens can be encoded by HLA-DP alleles. Indeed, matching for
HLA-DP antigens is generally not considered to be of functional
importance for the outcome of allogeneic bone marrow or peripheral
blood stem cell transplantation. In this study, a case of peripheral
blood stem cell allograft rejection was investigated in which the
patient and donor differed for a single mismatch at HLA-DP in the
rejection direction. Patient-derived T lymphocytes circulating at the
time of rejection showed direct ex vivo cytotoxic activity against
donor-derived B-lymphoblastoid cells as well as other
HLA-DP1*0901-expressing targets. The presence of
HLA-DP1*0901-specific effectors in vivo was further confirmed by in
vitro stimulation experiments. CD4+ T-cell lines and clones
with specific cytotoxic activity against HLA-DP1*0901-expressing
targets including donor B-lymphoblastoid cells were generated both by
nonspecific and by donor-specific in vitro stimulation. Taken together,
these data demonstrate that HLA-DP can be the target antigen of
cytotoxic CD4+ T lymphocytes involved in peripheral blood
stem cell allograft rejection.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. Crocchiolo, E. Zino, L. Vago, R. Oneto, B. Bruno, S. Pollichieni, N. Sacchi, M. P. Sormani, J. Marcon, T. Lamparelli, et al.
Nonpermissive HLA-DPB1 disparity is a significant independent risk factor for mortality after unrelated hematopoietic stem cell transplantation
Blood,
August 13, 2009;
114(7):
1437 - 1444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. E. Shaw, T. A. Gooley, M. Malkki, J. A. Madrigal, A. B. Begovich, M. M. Horowitz, A. Gratwohl, O. Ringden, S. G. E. Marsh, and E. W. Petersdorf
The importance of HLA-DPB1 in unrelated donor hematopoietic cell transplantation
Blood,
December 15, 2007;
110(13):
4560 - 4566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fleischhauer, F. Locatelli, M. Zecca, M. G. Orofino, C. Giardini, P. De Stefano, A. Pession, A. M. Iannone, C. Carcassi, E. Zino, et al.
Graft rejection after unrelated donor hematopoietic stem cell transplantation for thalassemia is associated with nonpermissive HLA-DPB1 disparity in host-versus-graft direction
Blood,
April 1, 2006;
107(7):
2984 - 2992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. E. Shaw, S. G.E. Marsh, N. P. Mayor, N. H. Russell, and J. A. Madrigal
HLA-DPB1 matching status has significant implications for recipients of unrelated donor stem cell transplants
Blood,
February 1, 2006;
107(3):
1220 - 1226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Zino, G. Frumento, S. Marktel, M. P. Sormani, F. Ficara, S. D. Terlizzi, A. M. Parodi, R. Sergeant, M. Martinetti, A. Bontadini, et al.
A T-cell epitope encoded by a subset of HLA-DPB1 alleles determines nonpermissive mismatches for hematologic stem cell transplantation
Blood,
February 15, 2004;
103(4):
1417 - 1424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
