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Blood, 15 August 2001, Vol. 98, No. 4, pp. 1127-1134
IMMUNOBIOLOGY
Gene expression screening of human mast cells and eosinophils
using high-density oligonucleotide probe arrays: abundant
expression of major basic protein in mast cells
Toshiharu Nakajima,
Kenji Matsumoto,
Hajime Suto,
Kazuko Tanaka,
Motohiro Ebisawa,
Hisashi Tomita,
Keisuke Yuki,
Toshio Katsunuma,
Akira Akasawa,
Ryoichi Hashida,
Yuji Sugita,
Hideoki Ogawa,
Chisei Ra, and
Hirohisa Saito
From the Department of Allergy & Immunology, National
Children's Medical Research Center, Tokyo, Japan; Genox Research,
Inc., Kawasaki, Kanagawa, Japan; Department of Pediatrics, National
Sagamihara Hospital, Japan; and Allergy Research Center, Juntendo
University School of Medicine, Tokyo, Japan.
Mast cells (MCs) and eosinophils are thought to play
important roles in evoking allergic inflammation. Cell-type-specific gene expression was screened among 12 000 genes in human MCs and eosinophils with the use of high-density oligonucleotide probe arrays.
In comparison with other leukocytes, MCs expressed 140 cell-type-specific transcripts, whereas eosinophils expressed only 34. Among the transcripts for expected MC-specific proteins such as
tryptase, major basic protein (MBP), which had been thought to be
eosinophil specific, was ranked fourth in terms of amounts of increased
MC-specific messenger RNA. Mature eosinophils were almost lacking this
transcript. MCs obtained from 4 different sources (ie, lung, skin,
adult peripheral blood progenitor-derived and cord blood
progenitor-derived MCs, and eosinophils) were found to have high
protein levels of MBP in their granules with the use of flow cytometric
and confocal laser scanning microscopic analyses. The present finding
that MCs can produce abundant MBP is crucial because many reports
regarding allergic pathogenesis have been based on earlier findings
that MBP was almost unique to eosinophils and not produced by MCs.
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