|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2001, Vol. 98, No. 4, pp. 925-933
CHEMOKINES
Blocking of c-FLIPL-independent
cycloheximide-induced apoptosis or Fas-mediated apoptosis by the CC
chemokine receptor 9/TECK interaction
Byung-S. Youn,
Young J. Kim,
Charlie Mantel,
Kang-Yeol Yu, and
Hal E. Broxmeyer
From the Department of Microbiology, Department of
Immunology and Medicine, and Department of Biochemistry and Molecular
Biology, Indiana University School of Medicine; and the Walther
Oncology Center, Indianapolis, IN 46202.
Chemokines play a pivotal role in regulating leukocyte migration as
well as other biological functions. CC chemokine receptor 9 (CCR9) is a
specific receptor for thymus-expressed CC chemokine (TECK). It
is shown here that engagement of CCR9 with TECK leads to
phosphorylation of Akt (protein kinase B), mitogen-activated protein kinases (MAPKs), glycogen synthase kinase-3 (GSK-3), and
a forkhead transcription factor, FKHR, in a human T-cell line, MOLT4, that naturally expresses CCR9. By means of chemical inhibitors, it is shown that phosphoinositide-3 kinase (PI-3 kinase), but not MAPK,
is required for CCR9-mediated chemotaxis. Akt, GSK-3, FKHR, and MAPK
have been previously implicated in cell survival signals in response to
an array of death stimuli. When MOLT4 cells, which expressed
Fas as well as CXCR4, were stimulated with cycloheximide (CHX), an
agonistic anti-Fas antibody, or a combination of these, the
cells rapidly underwent apoptosis. However, costimulation of MOLT4
cells with TECK or stromal derived factor-1 significantly blocked
CHX-mediated apoptosis, whereas stimulation only with TECK partially
blocked Fas-mediated apoptosis. Concomitant with this blocking,
cleavage of poly (adenosine 5'-diphosphate-ribose) polymerase and
activation of caspase 3 were significantly attenuated, but the
expression level of FLICE inhibitory protein c-FLIPL, which
had been shown to be regulated by CHX, was unchanged. This demonstrates
that activation of CCR9 leads to phosphorylation of GSK-3 and FKHR
and provides a cell survival signal to the receptor expressing cells
against CHX. It also suggests the existence of a novel pathway leading
to CHX-induced apoptosis independently of c-FLIPL.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
V. Levina, B. M. Nolen, A. M. Marrangoni, P. Cheng, J. R. Marks, M. J. Szczepanski, M. E. Szajnik, E. Gorelik, and A. E. Lokshin
Role of Eotaxin-1 Signaling in Ovarian Cancer
Clin. Cancer Res.,
April 15, 2009;
15(8):
2647 - 2656.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, H. Yuling, J. Yanping, T. Xinti, Y. Yaofang, Y. Feng, X. Ruijin, W. Li, C. Lang, L. Jingyi, et al.
CCL19 and CXCL13 Synergistically Regulate Interaction between B Cell Acute Lymphocytic Leukemia CD23+CD5+ B Cells and CD8+ T Cells
J. Immunol.,
September 1, 2007;
179(5):
2880 - 2888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chunsong, H. Yuling, W. Li, X. Jie, Z. Gang, Z. Qiuping, G. Qingping, Z. Kejian, Q. Li, A. E. Chang, et al.
CXC Chemokine Ligand 13 and CC Chemokine Ligand 19 Cooperatively Render Resistance to Apoptosis in B Cell Lineage Acute and Chronic Lymphocytic Leukemia CD23+CD5+ B Cells
J. Immunol.,
November 15, 2006;
177(10):
6713 - 6722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Qiuping, X. Jei, J. Youxin, J. Wei, L. Chun, W. Jin, W. Qun, L. Yan, H. Chunsong, Y. Mingzhen, et al.
CC Chemokine Ligand 25 Enhances Resistance to Apoptosis in CD4+ T Cells from Patients with T-Cell Lineage Acute and Chronic Lymphocytic Leukemia by Means of Livin Activation
Cancer Res.,
October 15, 2004;
64(20):
7579 - 7587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. E. Annels, A. J. Willemze, V. H. J. van der Velden, C. M. J. M. Faaij, E. van Wering, D. M. D. S. Sie-Go, R. M. Egeler, M. J. D. van Tol, and T. Revesz
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL
Blood,
April 1, 2004;
103(7):
2806 - 2808.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Schmitz, H. Weyd, A. Krueger, S. Baumann, S. C. Fas, P. H. Krammer, and S. Kirchhoff
Resistance of Short Term Activated T Cells to CD95-Mediated Apoptosis Correlates with De Novo Protein Synthesis of c-FLIPshort
J. Immunol.,
February 15, 2004;
172(4):
2194 - 2200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. H. Underhill, K. P. Kolli, and G. S. Kansas
Complexity within the plasma cell compartment of mice deficient in both E- and P-selectin: implications for plasma cell differentiation
Blood,
December 1, 2003;
102(12):
4076 - 4083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Qiuping, L. Qun, H. Chunsong, Z. Xiaolian, H. Baojun, Y. Mingzhen, L. Chengming, H. Jinshen, G. Qingping, Z. Kejian, et al.
Selectively Increased Expression and Functions of Chemokine Receptor CCR9 on CD4+ T Cells from Patients with T-Cell Lineage Acute Lymphocytic Leukemia
Cancer Res.,
October 1, 2003;
63(19):
6469 - 6477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Spinetti, G. Bernardini, G. Camarda, A. Mangoni, A. Santoni, M. C. Capogrossi, and M. Napolitano
The chemokine receptor CCR8 mediates rescue from dexamethasone-induced apoptosis via an ERK-dependent pathway
J. Leukoc. Biol.,
January 1, 2003;
73(1):
201 - 207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Li, M. S. Kluger, L. A. Madge, L. Zheng, A. L. M. Bothwell, and J. S. Pober
Interferon-{gamma} Augments CD95(APO-1/Fas) and Pro-Caspase-8 Expression and Sensitizes Human Vascular Endothelial Cells to CD95-Mediated Apoptosis
Am. J. Pathol.,
October 1, 2002;
161(4):
1485 - 1495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. B. Friedrich, S. Sinha, L. Li, S. Dedhar, T. Force, A. Rosenzweig, and R. E. Gerszten
Role of Integrin-linked Kinase in Leukocyte Recruitment
J. Biol. Chem.,
May 3, 2002;
277(19):
16371 - 16375.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Gerszten
Pleiotropic Effects of Chemokines in Vascular Lesion Development
Arterioscler Thromb Vasc Biol,
April 1, 2002;
22(4):
528 - 529.
[Full Text]
[PDF]
|
|
|
|
|
