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Blood, 15 September 2001, Vol. 98, No. 6, pp. 1963-1970
TRANSPLANTATION
Decreased immune functions of blood cells following mobilization
with granulocyte colony-stimulating factor: association with donor
characteristics
Shantaram S. Joshi,
James
C. Lynch,
Steve Z. Pavletic,
Stefano R. Tarantolo,
Samuel J. Pirruccello,
Anne Kessinger, and
Michael R. Bishop
From the Departments of Cell Biology and Anatomy,
Preventive and Societal Medicine, Internal Medicine, and Pathology and
Microbiology, University of Nebraska Medical Center, Omaha; and the
Department of Experimental Transplantation and Immunology, National
Cancer Institute, National Institutes of Health, Bethesda, MD.
In this study, mononuclear cells (MNCs) from granulocyte
colony-stimulating factor (G-CSF)-mobilized blood stem cell (BSC) harvests from 104 healthy donors were analyzed for their
immunological functions and compared with MNCs from 28 steady-state
nonmobilized donors. The relationships between donor characteristics
(age, gender, weight, and HLA type) and immune functions of the
harvests were also analyzed. There was a significant
(P < .01) decrease in natural killer and
lymphokine-activated killer (LAK) cell-mediated cytotoxicity for
G-CSF-mobilized effector cells compared with nonmobilized cells.
Similarly, there was a significant (P < .005) decrease
in both T-cell and B-cell mitogen response in G-CSF-mobilized cells
compared with nonmobilized cells. There was dose-dependent inhibition
of LAK cell-mediated cytotoxicity, but this effect was not seen with
other immune function assays. Changes in immune function did not appear
to be determined by frequency of cellular phenotypes or expression of
effector function genes seen in a reverse-transcription polymerase
chain reaction. There was a significant relationship between expression
of certain HLA alleles (A1, A3, A24, B44, B62, DR15, DR17; all
P < .01) and increased immune function, such as
cytotoxicity and/or mitogen response. A decrease in immune function
with the HLA-DR13 expression was also observed
(P < .01). Since the G-CSF increases the number of MNCs,
the increase in effector cells might compensate for decreased immune
functions of these cells in vivo when transplanted into patients. These results suggest a decreased immune function in G-CSF-mobilized BSC harvests and warrant further studies to correlate these data with clinical outcome.
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