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Related Collections Neoplasia Signal Transduction Brief Reports Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 November 2001, Vol. 98, No. 9, pp. 2853-2855 BRIEF REPORT The AKT kinase is activated in multiple myeloma tumor cells Jung-hsin Hsu, Yijiang Shi, Stanislaw Krajewski, Stephen Renner, Myrna Fisher, John C. Reed, Thomas F. Franke, and Alan Lichtenstein From the Department of Medicine and Pathology, West Los Angeles Veterans Administration Medical Center and Jonsson Comprehensive Cancer Center, Los Angeles, CA; Burnham Institute, La Jolla, CA; and the Department of Pharmacology, Columbia University, New York, NY. Immunohistochemistry (IHC) was performed on archived bone marrow (BM) with a phosphospecific anti-AKT antibody. IHC on 26 BM biopsies from patients with multiple myeloma (MM) demonstrated phospho-AKT staining of malignant plasma cells in a cell membrane-specific pattern, whereas nonmalignant hematopoietic cells did not stain. Preabsorption of the antibody with phosphorylated AKT peptide, but not nonphosphorylated peptide, abrogated staining. Frequency of plasma cell staining in BMs of patients with stage I or smoldering MM was significantly less than that of stage III MM marrows. Plasma cells in 10 patients with monoclonal gammopathy of undetermined significance were not stained by the antibody. To investigate the significance of AKT activation, 2 cell lines initiated from cultures of primary MM cells were also studied. Both demonstrated constitutive AKT activation. Interruption of AKT activation and activity, achieved by either exposure to wortmannin or by ectopic expression of a dominant negative AKT mutant, resulted in inhibition of MM cell growth in vitro. These results indicate that activation of the AKT kinase is a characteristic of MM cells and suggest that AKT activity is important for MM cell expansion. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: Immunohistochemical localization of phosphorylated AKT in multiple myeloma Serhan Alkan and Keith F. Izban Blood 2002 99: 2278-2279. [Full Text] [PDF] This article has been cited by other articles: P. Frost, Y. Shi, B. Hoang, J. Gera, and A. Lichtenstein Regulation of D-cyclin translation inhibition in myeloma cells treated with mammalian target of rapamycin inhibitors: rationale for combined treatment with extracellular signal-regulated kinase inhibitors and rapamycin Mol. Cancer Ther., January 1, 2009; 8(1): 83 - 93. [Abstract] [Full Text] [PDF] A. Zollinger, T. Stuhmer, M. Chatterjee, S. Gattenlohner, E. Haralambieva, H.-K. Muller-Hermelink, M. Andrulis, A. 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