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Blood, 1 November 2001, Vol. 98, No. 9, pp. 2872-2874
BRIEF REPORT
Erythropoietin expression in primary rat Sertoli and peritubular
myoid cells
Massimo Magnanti,
Orietta Gandini,
Laura Giuliani,
Paola Gazzaniga,
Hugo H. Marti,
Angela Gradilone,
Luigi Frati,
Anna Maria Aglianò, and
Max Gassmann
From the Department of Experimental Medicine and
Pathology, University of Rome "La Sapienza," Italy; and Institute
of Physiology, University of Zurich-Irchel, Zurich,
Switzerland.
Kidney and liver are the major organs of erythropoietin (Epo)
synthesis. However, Epo messenger RNA (mRNA) has been detected in
several organs, such as brain, lung, and testis. Furthermore, functional Epo receptors have been demonstrated on different cell types, including rat Leydig cells. The aim of the study was to identify
testicular cells expressing Epo mRNA and to quantitate its levels by
competitive reverse transcriptase-polymerase chain reaction (RT-PCR).
Besides whole testis, Epo transcripts were found in Sertoli and
peritubular myoid cells, while no signal was detected in Leydig cells.
Exposure of Sertoli cells to CoCl2 led to an increase of
Epo mRNA level. Semiquantitative competitive RT-PCR presented an
increase in the level of Epo mRNA in Sertoli cells stimulated by
follicle-stimulating hormone, while exposure of peritubular myoid cells
cultures to testosterone reduced Epo mRNA expression. Due to the
blood-testis barrier, basal expression of Epo suggests a not yet
defined function of this hormone in testis.
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