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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lu, Q. Articles by Richardson, B. Search for Related Content PubMed PubMed Citation Articles by Lu, Q. Articles by Richardson, B. Related Collections Immunobiology Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 June 2002, Vol. 99, No. 12, pp. 4503-4508 IMMUNOBIOLOGY Effect of DNA methylation and chromatin structure on ITGAL expression Qianjin Lu, Donna Ray, David Gutsch, and Bruce Richardson From the Department of Medicine, University of Michigan; and the Ann Arbor VA Hospital; both of Ann Arbor, MI. LFA-1 (CD11a/CD18, L2) is an integrin expressed in a tissue-specific fashion and is important in inflammatory and immune responses. Promoter analysis has identified transcription factors that may be involved in CD11a expression, but the mechanisms contributing to its tissue-specific expression are incompletely characterized. In this report we have asked if DNA methylation and/or chromatin structure could contribute to tissue-specific CD11a expression. Bisulfite sequencing was used to compare methylation patterns in the promoter and 5' flanking regions of the ITGAL gene, encoding CD11a, in normal human T cells, which express LFA-1, and fibroblasts, which do not. The region was found to be heavily methylated in fibroblasts but not T cells, and methylation correlated with an inactive chromatin configuration as analyzed by deoxyribonuclease 1 sensitivity. Patch methylation of the promoter region revealed that promoter activity was methylation-sensitive but that methylation of the 5' flanking regions more than 500 base pairs 5' to the transcription start site could also suppress promoter function. Treating fibroblasts with a DNA methylation inhibitor decreased ITGAL promoter methylation and increased CD11a messenger RNA. The results thus indicate that methylation and chromatin structure may contribute to the tissue-specific expression of CD11a. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Claus, M. Fliegauf, M. Stock, J. A. Duque, M. Kolanczyk, and M. Lubbert Inhibitors of DNA methylation and histone deacetylation independently relieve AML1/ETO-mediated lysozyme repression J. Leukoc. Biol., December 1, 2006; 80(6): 1462 - 1472. [Abstract] [Full Text] [PDF] K. Soda, Y. Kano, T. Nakamura, K. Kasono, M. Kawakami, and F. Konishi Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte J. Immunol., July 1, 2005; 175(1): 237 - 245. [Abstract] [Full Text] [PDF] S. Kanaji, T. Kanaji, B. Jacquelin, M. Chang, D. J. Nugent, N. Komatsu, M. Moroi, K. Izuhara, and T. J. Kunicki Thrombopoietin initiates demethylation-based transcription of GP6 during megakaryocyte differentiation Blood, May 15, 2005; 105(10): 3888 - 3892. [Abstract] [Full Text] [PDF] C. G. Kevil, M. J. Hicks, X. He, J. Zhang, C. M. Ballantyne, C. Raman, T. R. Schoeb, and D. C. Bullard Loss of LFA-1, but not Mac-1, Protects MRL/MpJ-Faslpr Mice from Autoimmune Disease Am. J. Pathol., August 1, 2004; 165(2): 609 - 616. [Abstract] [Full Text] [PDF] M. J. Kaplan, Q. Lu, A. Wu, J. Attwood, and B. Richardson Demethylation of Promoter Regulatory Elements Contributes to Perforin Overexpression in CD4+ Lupus T Cells J. Immunol., March 15, 2004; 172(6): 3652 - 3661. [Abstract] [Full Text] [PDF] A. Puig-Kroger, T. Sanchez-Elsner, N. Ruiz, E. J. Andreu, F. Prosper, U. B. Jensen, J. Gil, P. Erickson, H. Drabkin, Y. Groner, et al. RUNX/AML and C/EBP factors regulate CD11a integrin expression in myeloid cells through overlapping regulatory elements Blood, November 1, 2003; 102(9): 3252 - 3261. [Abstract] [Full Text] [PDF] N. Sato, A. Maitra, N. Fukushima, N. T. van Heek, H. Matsubayashi, C. A. Iacobuzio-Donahue, C. Rosty, and M. Goggins Frequent Hypomethylation of Multiple Genes Overexpressed in Pancreatic Ductal Adenocarcinoma Cancer Res., July 15, 2003; 63(14): 4158 - 4166. [Abstract] [Full Text] [PDF] Q. Lu, A. Wu, D. Ray, C. Deng, J. Attwood, S. Hanash, M. Pipkin, M. Lichtenheld, and B. Richardson DNA Methylation and Chromatin Structure Regulate T Cell Perforin Gene Expression J. Immunol., May 15, 2003; 170(10): 5124 - 5132. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020