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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by McLellan, A. D. Articles by Kämpgen, E. Search for Related Content PubMed PubMed Citation Articles by McLellan, A. D. Articles by Kämpgen, E. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 March 2002, Vol. 99, No. 6, pp. 2084-2093 IMMUNOBIOLOGY Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression Alexander D. McLellan, Michaela Kapp, Andreas Eggert, Christian Linden, Ursula Bommhardt, Eva-B. Bröcker, Ulrike Kämmerer, and Eckhart Kämpgen From the Department of Dermatology, Obstetrics and Gynecology, and Virology, University of Würzburg, Würzburg, Germany. Mouse spleen contains CD4+, CD8+, and CD4/CD8 dendritic cells (DCs) in a 2:1:1 ratio. An analysis of 70 surface and cytoplasmic antigens revealed several differences in antigen expression between the 3 subsets. Notably, the Birbeck granule-associated Langerin antigen, as well as CD103 (the mouse homologue of the rat DC marker OX62), were specifically expressed by the CD8+ DC subset. All DC types were apparent in the T-cell areas as well as in the splenic marginal zones and showed similar migratory capacity in collagen lattices. The 3 DC subtypes stimulated allogeneic CD4+ T cells comparably. However, CD8+ DCs were very weak stimulators of resting or activated allogeneic CD8+ T cells, even at high stimulator-to-responder ratios, although this defect could be overcome under optimal DC/T cell ratios and peptide concentrations using CD8+ F5 T-cell receptor (TCR)-transgenic T cells. CD8 or CD8+ DCs presented alloantigens with the same efficiency for lysis by cytotoxic T lymphocytes (CTLs), and their turnover rate of class I-peptide complexes was similar, thus neither an inability to present, nor rapid loss of antigenic complexes from CD8 DCs was responsible for the low allostimulatory capacity of CD8+ DCs in vitro. Surprisingly, both CD8+ DCs and CD4/CD8 DCs efficiently primed minor histocompatibility (H-Y male antigen) cytotoxicity following intravenous injection, whereas CD4+ DCs were weak inducers of CTLs. Thus, the inability of CD8+ DCs to stimulate CD8+ T cells is limited to certain in vitro assays that must lack certain enhancing signals present during in vivo interaction between CD8+ DCs and CD8+ T cells. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Piccioli, C. Sammicheli, S. Tavarini, S. Nuti, E. Frigimelica, A. G.O. Manetti, A. Nuccitelli, S. Aprea, S. Valentini, E. Borgogni, et al. Human plasmacytoid dendritic cells are unresponsive to bacterial stimulation and require a novel type of cooperation with myeloid dendritic cells for maturation Blood, April 30, 2009; 113(18): 4232 - 4239. [Abstract] [Full Text] [PDF] J. Idoyaga, N. Suda, K. Suda, C. G. Park, and R. M. Steinman Antibody to Langerin/CD207 localizes large numbers of CD8{alpha}+ dendritic cells to the marginal zone of mouse spleen PNAS, February 3, 2009; 106(5): 1524 - 1529. 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