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Blood, 1 April 2002, Vol. 99, No. 7, pp. 2459-2467
IMMUNOBIOLOGY
Differential surrogate light chain expression governs B-cell
differentiation
Yui-Hsi Wang,
Robert P. Stephan,
Alexander Scheffold,
Désirée Kunkel,
Hajime Karasuyama,
Andreas Radbruch, and
Max D. Cooper
From the Departments of Medicine, Pediatrics, and
Microbiology, Division of Developmental and Clinical Immunology,
University of Alabama at Birmingham and the Howard Hughes Medical
Institute, Birmingham, AL; Deutsches Rheuma-Forschungszentrum, Berlin,
Germany; and the Department of Immune Regulation, Tokyo Medical and
Dental University, Japan.
Surrogate light chain expression during B lineage differentiation
was examined by using indicator fluorochrome-filled liposomes in an
enhanced immunofluorescence assay. Pro-B cells bearing surrogate light
chain components were found in mice, but not in humans. A limited
subpopulation of relatively large pre-B cells in both species expressed
pre-B cell receptors. These cells had reduced expression of the
recombinase activating genes, RAG-1 and RAG-2. Their receptor-negative pre-B cell progeny were relatively small, expressed RAG-1 and RAG-2, and exhibited
selective down-regulation of VpreB and  5
expression. Comparative analysis of the 2 pre-B cell
subpopulations indicated that loss of the pre-B cell receptors from surrogate light chain gene silencing was linked with exit from the
cell cycle and light chain gene rearrangement to achieve B-cell differentiation.
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