SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Price, M. O. Articles by Dinauer, M. C. Search for Related Content PubMed PubMed Citation Articles by Price, M. O. Articles by Dinauer, M. C. Related Collections Phagocytes Plenary Papers Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 April 2002, Vol. 99, No. 8, pp. 2653-2661 PLENARY PAPER Creation of a genetic system for analysis of the phagocyte respiratory burst: high-level reconstitution of the NADPH oxidase in a nonhematopoietic system Marianne O. Price, Linda C. McPhail, J. David Lambeth, Chang-Hoon Han, Ulla G. Knaus, and Mary C. Dinauer From the Herman B Wells Center for Pediatric Research, Department of Pediatrics (Hematology/Oncology) and Medical and Molecular Genetics, James Whitcomb Riley Hospital for Children, Indiana University Medical Center, Indianapolis; Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, NC; Department of Biochemistry, Emory University Medical School, Atlanta, GA; Department of Immunology, The Scripps Research Institute, La Jolla, CA. The phagocyte nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH) oxidase was functionally reconstituted in monkey kidney COS-7 cells by transfection of essential subunits, gp91phox, p22phox, p47phox, and p67phox. COS-7 cells express the essential small guanosine 5'-triphosphatase, Rac1. Transgenic COS-phox cells were capable of arachidonic acid-induced NADPH oxidase activity up to 80% of that of human neutrophils, and of phorbol myristate acetate (PMA)-induced activity up to 20% of that of neutrophils. Expression of all 4 phox components was required for enzyme activity, and enzyme activation was associated with membrane translocation of p47phox, p67phox, and Rac1. Expression of p47phox Ser303Ala/Ser304Ala or Ser379Ala phosphorylation-deficient mutants resulted in significantly impaired NAPDH oxidase activity, compared with expression of wild-type p47phox or the p47phox Ser303Glu/Ser304Glu phosphorylation mimic, suggesting that p47phox phosphorylation contributes to enzyme activity in the COS system, as is the case in neutrophils. Hence, COS-phox cells should be useful as a new whole-cell model that is both capable of high-level superoxide production and readily amenable to genetic manipulation for investigation of NADPH oxidase function. PMA-elicited superoxide production in COS-phox cells was regulated by activation of protein kinase C (PKC) and Rac. Although COS-7 cells differ from human neutrophils in PKC isoform expression, transient expression of major neutrophil isoforms in COS-phox cells did not increase PMA-induced superoxide production, suggesting that endogenous isoforms were not rate limiting. Val204 in p67phox, previously shown to be required for NADPH oxidase activity under cell-free conditions, was found to be essential for superoxide production by intact COS-phox cells, on the basis of transfection studies using a p67phox (Val204Ala) mutant. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. D. Matute, A. A. Arias, N. A. M. Wright, I. Wrobel, C. C. M. Waterhouse, X. J. Li, C. C. Marchal, N. D. Stull, D. B. Lewis, M. Steele, et al. A new genetic subgroup of chronic granulomatous disease with autosomal recessive mutations in p40phox and selective defects in neutrophil NADPH oxidase activity Blood, October 8, 2009; 114(15): 3309 - 3315. [Abstract] [Full Text] [PDF] R. D. Ye, F. Boulay, J. M. Wang, C. Dahlgren, C. Gerard, M. Parmentier, C. N. Serhan, and P. M. Murphy International Union of Basic and Clinical Pharmacology. LXXIII. Nomenclature for the Formyl Peptide Receptor (FPR) Family Pharmacol. Rev., June 1, 2009; 61(2): 119 - 161. [Abstract] [Full Text] [PDF] I. S. Ramsey, E. Ruchti, J. S. Kaczmarek, and D. E. Clapham Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst PNAS, May 5, 2009; 106(18): 7642 - 7647. [Abstract] [Full Text] [PDF] A.-J. Casbon, L.-A. H. Allen, K. W. Dunn, and M. C. Dinauer Macrophage NADPH Oxidase Flavocytochrome b Localizes to the Plasma Membrane and Rab11-Positive Recycling Endosomes J. Immunol., February 15, 2009; 182(4): 2325 - 2339. [Abstract] [Full Text] [PDF] W. Tian, X. J. Li, N. D. Stull, W. Ming, C.-I. Suh, S. A. Bissonnette, M. B. Yaffe, S. Grinstein, S. J. Atkinson, and M. C. Dinauer Fc{gamma}R-stimulated activation of the NADPH oxidase: phosphoinositide-binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the phagosome Blood, November 1, 2008; 112(9): 3867 - 3877. [Abstract] [Full Text] [PDF] Y.-Y. Kao, D. Gianni, B. Bohl, R. M. Taylor, and G. M. Bokoch Identification of a Conserved Rac-binding Site on NADPH Oxidases Supports a Direct GTPase Regulatory Mechanism J. Biol. Chem., May 9, 2008; 283(19): 12736 - 12746. [Abstract] [Full Text] [PDF] G. Hu, R. D. Ye, M. C. Dinauer, A. B. Malik, and R. D. Minshall Neutrophil caveolin-1 expression contributes to mechanism of lung inflammation and injury Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L178 - L186. [Abstract] [Full Text] [PDF] H. Choi, T. L. Leto, L. Hunyady, K. J. Catt, Y. S. Bae, and S. G. Rhee Mechanism of Angiotensin II-induced Superoxide Production in Cells Reconstituted with Angiotensin Type 1 Receptor and the Components of NADPH Oxidase J. Biol. Chem., January 4, 2008; 283(1): 255 - 267. [Abstract] [Full Text] [PDF] N. Cheng, R. He, J. Tian, M. C. Dinauer, and R. D. Ye A Critical Role of Protein Kinase C{delta} Activation Loop Phosphorylation in Formyl-Methionyl-Leucyl-Phenylalanine-Induced Phosphorylation of p47phox and Rapid Activation of Nicotinamide Adenine Dinucleotide Phosphate Oxidase J. Immunol., December 1, 2007; 179(11): 7720 - 7728. [Abstract] [Full Text] [PDF] J. Chen, R. He, R. D. Minshall, M. C. Dinauer, and R. D. Ye Characterization of a Mutation in the Phox Homology Domain of the NADPH Oxidase Component p40phox Identifies A Mechanism for Negative Regulation of Superoxide Production J. Biol. Chem., October 12, 2007; 282(41): 30273 - 30284. [Abstract] [Full Text] [PDF] W. Ming, S. Li, D. D. Billadeau, L. A. Quilliam, and M. C. Dinauer The Rac Effector p67phox Regulates Phagocyte NADPH Oxidase by Stimulating Vav1 Guanine Nucleotide Exchange Activity Mol. Cell. Biol., January 1, 2007; 27(1): 312 - 323. [Abstract] [Full Text] [PDF] C.-I. Suh, N. D. Stull, X. J. Li, W. Tian, M. O. Price, S. Grinstein, M. B. Yaffe, S. Atkinson, and M. C. Dinauer The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc{gamma}IIA receptor-induced phagocytosis J. Exp. Med., August 7, 2006; 203(8): 1915 - 1925. [Abstract] [Full Text] [PDF] K. Miyano, N. Ueno, R. Takeya, and H. Sumimoto Direct Involvement of the Small GTPase Rac in Activation of the Superoxide-producing NADPH Oxidase Nox1 J. Biol. Chem., August 4, 2006; 281(31): 21857 - 21868. [Abstract] [Full Text] [PDF] B. Wilkinson, J. Koenigsknecht-Talboo, C. Grommes, C. Y. D. Lee, and G. Landreth Fibrillar beta-Amyloid-stimulated Intracellular Signaling Cascades Require Vav for Induction of Respiratory Burst and Phagocytosis in Monocytes and Microglia J. Biol. Chem., July 28, 2006; 281(30): 20842 - 20850. [Abstract] [Full Text] [PDF] C. Marty, T. Kozasa, M. T. Quinn, and R. D. Ye Activation State-Dependent Interaction between G{alpha}i and p67phox. Mol. Cell. Biol., July 1, 2006; 26(13): 5190 - 5200. [Abstract] [Full Text] [PDF] P. L. Hordijk Regulation of NADPH Oxidases: The Role of Rac Proteins Circ. Res., March 3, 2006; 98(4): 453 - 462. [Abstract] [Full Text] [PDF] J. Alblas, H. Honing, C. Renardel de Lavalette, M. H. Brown, C. D. Dijkstra, and T. K. van den Berg Signal Regulatory Protein {alpha} Ligation Induces Macrophage Nitric Oxide Production through JAK/STAT- and Phosphatidylinositol 3-Kinase/Rac1/NAPDH Oxidase/H2O2-Dependent Pathways Mol. Cell. Biol., August 15, 2005; 25(16): 7181 - 7192. [Abstract] [Full Text] [PDF] N. Ueno, R. Takeya, K. Miyano, H. Kikuchi, and H. Sumimoto The NADPH Oxidase Nox3 Constitutively Produces Superoxide in a p22phox-dependent Manner: ITS REGULATION BY OXIDASE ORGANIZERS AND ACTIVATORS J. Biol. Chem., June 17, 2005; 280(24): 23328 - 23339. [Abstract] [Full Text] [PDF] R. He, M. Nanamori, H. Sang, H. Yin, M. C. Dinauer, and R. D. Ye Reconstitution of Chemotactic Peptide-Induced Nicotinamide Adenine Dinucleotide Phosphate (Reduced) Oxidase Activation in Transgenic COS-phox Cells J. Immunol., December 15, 2004; 173(12): 7462 - 7470. [Abstract] [Full Text] [PDF] G. Cheng, D. Ritsick, and J. D. Lambeth Nox3 Regulation by NOXO1, p47phox, and p67phox J. Biol. Chem., August 13, 2004; 279(33): 34250 - 34255. [Abstract] [Full Text] [PDF] B. A. Diebold, B. Fowler, J. Lu, M. C. Dinauer, and G. M. Bokoch Antagonistic Cross-talk between Rac and Cdc42 GTPases Regulates Generation of Reactive Oxygen Species J. Biol. Chem., July 2, 2004; 279(27): 28136 - 28142. [Abstract] [Full Text] [PDF] R. Takeya, N. Ueno, K. Kami, M. Taura, M. Kohjima, T. Izaki, H. Nunoi, and H. Sumimoto Novel Human Homologues of p47phox and p67phox Participate in Activation of Superoxide-producing NADPH Oxidases J. Biol. Chem., June 27, 2003; 278(27): 25234 - 25246. [Abstract] [Full Text] [PDF] Y. Gu, Y. C. Xu, R. F. Wu, F. E. Nwariaku, R. F. Souza, S. C. Flores, and L. S. Terada p47phox Participates in Activation of RelA in Endothelial Cells J. Biol. Chem., May 2, 2003; 278(19): 17210 - 17217. [Abstract] [Full Text] [PDF] T. E. Decoursey Voltage-Gated Proton Channels and Other Proton Transfer Pathways Physiol Rev, April 1, 2003; 83(2): 475 - 579. [Abstract] [Full Text] [PDF] B. Banfi, R. A. Clark, K. Steger, and K.-H. Krause Two Novel Proteins Activate Superoxide Generation by the NADPH Oxidase NOX1 J. Biol. Chem., January 31, 2003; 278(6): 3510 - 3513. [Abstract] [Full Text] [PDF] N. Touret and S. Grinstein Voltage-gated Proton "Channels": a Spectator's Viewpoint J. Gen. Physiol., November 25, 2002; 120(6): 767 - 771. [Full Text] [PDF] A. Maturana, K.-H. Krause, and N. Demaurex NOX Family NADPH Oxidases: Do They Have Built-in Proton Channels? J. Gen. Physiol., November 25, 2002; 120(6): 781 - 786. [Full Text] [PDF] K. J. Biberstine-Kinkade, L. Yu, N. Stull, B. LeRoy, S. Bennett, A. Cross, and M. C. Dinauer Mutagenesis of p22phox Histidine 94. A HISTIDINE IN THIS POSITION IS NOT REQUIRED FOR FLAVOCYTOCHROME b558 FUNCTION J. Biol. Chem., August 9, 2002; 277(33): 30368 - 30374. [Abstract] [Full Text] [PDF] D. Morgan, V. V. Cherny, M. O. Price, M. C. Dinauer, and T. E. DeCoursey Absence of Proton Channels in COS-7 Cells Expressing Functional NADPH Oxidase Components J. Gen. Physiol., June 1, 2002; 119(6): 571 - 580. [Abstract] [Full Text] [PDF] M. O. Price, S. J. Atkinson, U. G. Knaus, and M. C. Dinauer Rac Activation Induces NADPH Oxidase Activity in Transgenic COSphox Cells, and the Level of Superoxide Production Is Exchange Factor-dependent J. Biol. Chem., May 17, 2002; 277(21): 19220 - 19228. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020