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Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 May 2002, Vol. 99, No. 9, pp. 3241-3249 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY A mouse orthologue of puromycin-insensitive leucyl-specific aminopeptidase is expressed in endothelial cells and plays an important role in angiogenesis Hiroki Miyashita, Tohru Yamazaki, Tetsuya Akada, Osamu Niizeki, Minetaro Ogawa, Shin-ichi Nishikawa, and Yasufumi Sato From the Department of Vascular Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan; and the Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Japan. Using polymerase chain reaction-coupled subtractive hybridization, we have isolated genes expressed during the in vitro differentiation of murine embryonic stem cells into endothelial cells (ECs). Among the genes obtained, we identified one gene that was inducible by vascular endothelial growth factor (VEGF) in the murine EC line MSS31. Analysis of the nucleotide and deduced amino acid sequences revealed that the protein was composed of 930 amino acids, including an HEXXH(X)18E consensus sequence of the M1 aminopeptidase family, and is thought to be a mouse orthologue of puromycin-insensitive leucyl-specific aminopeptidase (mPILSAP). The recombinant protein hydrolyzed N-terminal leucyl and methionyl residues from synthetic substrates. Immunohistochemical analysis revealed that mPILSAP was expressed in ECs during postnatal angiogenesis. Specific elimination of mPILSAP expression by antisense oligodeoxynucleotide (AS-ODN) attenuated VEGF-stimulated proliferation, migration, and network formation of ECs in vitro. Moreover, AS-ODN to mPILSAP inhibited angiogenesis in vivo. These results suggest a novel function of mPILSAP, which is expressed in ECs and plays an important role in angiogenesis. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Maruyama, A. Hattori, Y. Goto, M. Ueda, M. Maeda, H. Fujiwara, and M. Tsujimoto Laeverin/Aminopeptidase Q, a Novel Bestatin-sensitive Leucine Aminopeptidase Belonging to the M1 Family of Aminopeptidases J. Biol. Chem., July 13, 2007; 282(28): 20088 - 20096. [Abstract] [Full Text] [PDF] M. Abe and Y. Sato Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) is required for the development of vascular as well as hematopoietic system in embryoid bodies. Genes Cells, July 1, 2006; 11(7): 719 - 729. [Abstract] [Full Text] [PDF] V. W.M. van Hinsbergh, M. A. Engelse, and P. H.A. 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