|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 May 2002, Vol. 99, No. 9, pp. 3319-3325
IMMUNOBIOLOGY
Human primary and memory cytotoxic T lymphocyte responses are
efficiently induced by means of CD40-activated B cells as
antigen-presenting cells: potential for clinical
application
Michael S. von Bergwelt-Baildon,
Robert H. Vonderheide,
Britta Maecker,
Naoto Hirano,
Karen S. Anderson,
Marcus O. Butler,
Zhinan Xia,
Wan Y. Zeng,
Kai W. Wucherpfennig,
Lee M. Nadler, and
Joachim L. Schultze
From the Department of Adult Oncology and the
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute;
Department of Medicine, Harvard Medical School; and the Department of
Medicine, Brigham and Women's Hospital, Boston, MA.
CD40 engagement is the major signal that induces B cells to
efficiently present antigen to T cells. We previously demonstrated that
human peripheral blood-derived CD40-activated B cells (CD40-B cells)
function as antigen-presenting cells (APCs). Here, we have established
a culture system to generate these APCs under clinically applicable
conditions using guanylic acid-grade soluble trimeric CD40
ligand. To monitor APC function and antigen loading for these cells,
simple and efficient quality control assays have been developed. Using
this approach, we demonstrate that CD40-B cells from healthy donors
and cancer patients are fully functional and equally expanded in
long-term cultures. These B cells boost robust memory T-cell responses,
but more importantly, they also prime naive T-cell responses against
neoantigens ex vivo. CD40-B cells overcome current obstacles,
such as the difficulty of isolation, generation, and long-term
expansion observed with other APCs. Therefore, they are an excellent
source of professional APCs for immune assessment, antigen
discovery, and antigen-specific immunotherapy.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Q. Li, S. Teitz-Tennenbaum, E. J. Donald, M. Li, and A. E. Chang
In Vivo Sensitized and In Vitro Activated B Cells Mediate Tumor Regression in Cancer Adoptive Immunotherapy
J. Immunol.,
September 1, 2009;
183(5):
3195 - 3203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Shimabukuro-Vornhagen, E. Kondo, T. Liebig, and M. von Bergwelt-Baildon
Activated human B cells: stimulatory or tolerogenic antigen-presenting cells?
Blood,
July 16, 2009;
114(3):
746 - 747.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Tu, J. Zheng, Y. Liu, and Y.-L. Lau
Response: Stimulatory or tolerogenic role of CD40-activated B cells depends on the strength of the activation to T cells
Blood,
July 16, 2009;
114(3):
747 - 748.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Carpenter, R. Mick, A. J. Rech, G. L. Beatty, T. A. Colligon, M. R. Rosenfeld, D. E. Kaplan, K.-M. Chang, S. M. Domchek, P. A. Kanetsky, et al.
Collapse of the CD27+ B-Cell Compartment Associated with Systemic Plasmacytosis in Patients with Advanced Melanoma and Other Cancers
Clin. Cancer Res.,
July 1, 2009;
15(13):
4277 - 4287.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Kondo, B. Maecker, M. R. Weihrauch, C. Wickenhauser, W. Zeng, L. M. Nadler, J. L. Schultze, and M. S. von Bergwelt-Baildon
Cyclin D1-Specific Cytotoxic T Lymphocytes Are Present in the Repertoire of Cancer Patients: Implications for Cancer Immunotherapy
Clin. Cancer Res.,
October 15, 2008;
14(20):
6574 - 6579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Tu, Y.-L. Lau, J. Zheng, Y. Liu, P.-L. Chan, H. Mao, K. Dionis, P. Schneider, and D. B. Lewis
Efficient generation of human alloantigen-specific CD4+ regulatory T cells from naive precursors by CD40-activated B cells
Blood,
September 15, 2008;
112(6):
2554 - 2562.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Shimabukuro-Vornhagen, T. Liebig, and M. von Bergwelt-Baildon
Statins inhibit human APC function: implications for the treatment of GVHD
Blood,
August 15, 2008;
112(4):
1544 - 1545.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sarantopoulos, K. E. Stevenson, H. T. Kim, N. S. Bhuiya, C. S. Cutler, R. J. Soiffer, J. H. Antin, and J. Ritz
High Levels of B-Cell Activating Factor in Patients with Active Chronic Graft-Versus-Host Disease
Clin. Cancer Res.,
October 15, 2007;
13(20):
6107 - 6114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhang, K. M. Snyder, M. M. Suhoski, M. V. Maus, V. Kapoor, C. H. June, and C. L. Mackall
4-1BB Is Superior to CD28 Costimulation for Generating CD8+ Cytotoxic Lymphocytes for Adoptive Immunotherapy
J. Immunol.,
October 1, 2007;
179(7):
4910 - 4918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Leclerc, D. Beauseigle, J. Denis, H. Morin, C. Pare, A. Lamarre, and R. Lapointe
Proteasome-Independent Major Histocompatibility Complex Class I Cross-Presentation Mediated by Papaya Mosaic Virus-Like Particles Leads to Expansion of Specific Human T Cells
J. Virol.,
February 1, 2007;
81(3):
1319 - 1326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. Stone, S. Barzee, V. Snarsky, C. A. Spina, J. D. Lifson, V. K. B. Pillai, R. R. Amara, F. Villinger, and R. S. Kornbluth
Macaque Multimeric Soluble CD40 Ligand and GITR Ligand Constructs Are Immunostimulatory Molecules In Vitro
Clin. Vaccine Immunol.,
November 1, 2006;
13(11):
1223 - 1230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Tang, M. Murtadha, M. Schnell, L. C. Eisenlohr, J. Hooper, and P. Flomenberg
Human T-cell responses to vaccinia virus envelope proteins.
J. Virol.,
October 1, 2006;
80(20):
10010 - 10020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Chung, B.-S. Kim, Y.-J. Kim, H.-J. Ko, S.-Y. Ko, D.-H. Kim, and C.-Y. Kang
CD1d-Restricted T Cells License B Cells to Generate Long-Lasting Cytotoxic Antitumor Immunity In vivo.
Cancer Res.,
July 1, 2006;
66(13):
6843 - 6850.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. von Bergwelt-Baildon, A. Shimabukuro-Vornhagen, A. Popov, N. Klein-Gonzalez, F. Fiore, S. Debey, A. Draube, B. Maecker, I. Menezes, L. M. Nadler, et al.
CD40-activated B cells express full lymph node homing triad and induce T-cell chemotaxis: potential as cellular adjuvants
Blood,
April 1, 2006;
107(7):
2786 - 2789.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Hirano, M. O. Butler, Z. Xia, S. Ansen, M. S. von Bergwelt-Baildon, D. Neuberg, G. J. Freeman, and L. M. Nadler
Engagement of CD83 ligand induces prolonged expansion of CD8+ T cells and preferential enrichment for antigen specificity
Blood,
February 15, 2006;
107(4):
1528 - 1536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. F. Rousseau, E. Biagi, A. Dutour, E. S. Yvon, M. P. Brown, T. Lin, Z. Mei, B. Grilley, E. Popek, H. E. Heslop, et al.
Immunotherapy of high-risk acute leukemia with a recipient (autologous) vaccine expressing transgenic human CD40L and IL-2 after chemotherapy and allogeneic stem cell transplantation
Blood,
February 15, 2006;
107(4):
1332 - 1341.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Weihrauch, S. Ansen, E. Jurkiewicz, C. Geisen, Z. Xia, K. S. Anderson, E. Gracien, M. Schmidt, B. Wittig, V. Diehl, et al.
Phase I/II Combined Chemoimmunotherapy with Carcinoembryonic Antigen-Derived HLA-A2-Restricted CAP-1 Peptide and Irinotecan, 5-Fluorouracil, and Leucovorin in Patients with Primary Metastatic Colorectal Cancer
Clin. Cancer Res.,
August 15, 2005;
11(16):
5993 - 6001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Hoogendoorn, J. Olde Wolbers, W. M. Smit, M. R. Schaafsma, I. Jedema, R. M.Y. Barge, R. Willemze, and J.H. F. Falkenburg
Primary Allogeneic T-Cell Responses against Mantle Cell Lymphoma Antigen-Presenting Cells for Adoptive Immunotherapy after Stem Cell Transplantation
Clin. Cancer Res.,
July 15, 2005;
11(14):
5310 - 5318.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Fujiwara, J. J. Melenhorst, F. El Ouriaghli, S. Kajigaya, M. Grube, G. Sconocchia, K. Rezvani, D. A. Price, N. F. Hensel, D. C. Douek, et al.
In vitro Induction of Myeloid Leukemia-Specific CD4 and CD8 T Cells by CD40 Ligand - Activated B Cells Gene Modified to Express Primary Granule Proteins
Clin. Cancer Res.,
June 15, 2005;
11(12):
4495 - 4503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. N. Haining, D. G. Anderson, S. R. Little, M. S. von Berwelt-Baildon, A. A. Cardoso, P. Alves, K. Kosmatopoulos, L. M. Nadler, R. Langer, and D. S. Kohane
pH-Triggered Microparticles for Peptide Vaccination
J. Immunol.,
August 15, 2004;
173(4):
2578 - 2585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Palena, M. Zhu, J. Schlom, and K.-Y. Tsang
Human B cells that hyperexpress a triad of costimulatory molecules via avipox-vector infection: an alternative source of efficient antigen-presenting cells
Blood,
July 1, 2004;
104(1):
192 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. von Bergwelt-Baildon, B. Maecker, J. Schultze, and J. G. Gribben
CD40 activation: potential for specific immunotherapy in B-CLL
Ann. Onc.,
June 1, 2004;
15(6):
853 - 857.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. von Bergwelt-Baildon, J. L. Schultze, B. Maecker, I. Menezes, L. M. Nadler, R. Lapointe, J. Thibodeau, and P. Hwu
Correspondence re R. Lapointe et al., CD40-stimulated B Lymphocytes Pulsed with Tumor Antigens Are Effective Antigen-presenting Cells That Can Generate Specific T Cells. Cancer Res 2003;63:2836-43.
Cancer Res.,
June 1, 2004;
64(11):
4055 - 4057.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Y. Mapara and M. Sykes
Tolerance and Cancer: Mechanisms of Tumor Evasion and Strategies for Breaking Tolerance
J. Clin. Oncol.,
March 15, 2004;
22(6):
1136 - 1151.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Coughlin, B. A. Vance, S. A. Grupp, and R. H. Vonderheide
RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy
Blood,
March 15, 2004;
103(6):
2046 - 2054.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Takahashi, J. P. McCoy Jr, C. Carvallo, C. Rivera, T. Igarashi, R. Srinivasan, N. S. Young, and R. W. Childs
In vitro and in vivo evidence of PNH cell sensitivity to immune attack after nonmyeloablative allogeneic hematopoietic cell transplantation
Blood,
February 15, 2004;
103(4):
1383 - 1390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Wagner, H. Poeck, B. Jahrsdoerfer, S. Rothenfusser, D. Prell, B. Bohle, E. Tuma, T. Giese, J. W. Ellwart, S. Endres, et al.
IL-12p70-Dependent Th1 Induction by Human B Cells Requires Combined Activation with CD40 Ligand and CpG DNA
J. Immunol.,
January 15, 2004;
172(2):
954 - 963.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Maecker, D. H. Sherr, R. H. Vonderheide, M. S. von Bergwelt-Baildon, N. Hirano, K. S. Anderson, Z. Xia, M. O. Butler, K. W. Wucherpfennig, C. O'Hara, et al.
The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells
Blood,
November 1, 2003;
102(9):
3287 - 3294.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
