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Blood, 1 September 2006, Vol. 108, No. 5, pp. 1770-1773. Prepublished online as a Blood First Edition Paper on May 4, 2006; DOI 10.1182/blood-2005-10-006536. This Article Full Text (PDF) All Versions of this Article: blood-2005-10-006536v1 108/5/1770    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Park, K.-D. Articles by Szabolcs, P. Search for Related Content PubMed PubMed Citation Articles by Park, K.-D. Articles by Szabolcs, P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 25, 2005 Accepted April 18, 2006 In vitro priming and expansion of cytomegalovirus- specific Th1 and Tc1 T cells from naive Cord Blood lymphocytes Kyung-Duk Park, Luciana Marti, Joanne Kurtzberg, and Paul Szabolcs* Department of Pediatrics, Pediatric Blood and Marrow Transplant Program, Duke University, Durham, NC * Corresponding author; email: szabo001{at}mc.duke.edu. Adoptive transfer of CMV-specific cytotoxic T cells (CTL) expanded in vitro from memory donor T cells can reduce the incidence of CMV disease in allogeneic transplant recipients. However, this approach has been unavailable in the cord blood (CB) transplant setting because CB T cells are antigen-naïve and biased towards Th2/Tc2 function. We developed a protocol to in vitro prime and expand CMV-specific CTL from CB. T cells were primed with cytokines to trigger skewing towards Th1/Tc1 lineage before encountering monocyte and CD34+ progenitor-derived dendritic cells loaded with CMV-antigen and its immune complex. CMV-pulsed cultures expanded significantly more over 4-6 weeks than CMV- cultures despite identical cytokine milieu. T cells isolated from CMV+ cultures showed a preferential expansion of CD45RA-/RO+/CD27+ T cells compared to CMV- cultures. CMV-specific IFN and TNF producing CD4+ (Th1) and CD8+ (Tc1) T cells were enriched after 3-4 weeks and CMV-specific cytotoxicity developed 1-2 weeks later. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. W. Childs and C. S. Zerbe Expanding multiviral reactive T cells from cord blood Blood, August 27, 2009; 114(9): 1725 - 1726. [Abstract] [Full Text] [PDF] P. J. Hanley, C. R. Y. Cruz, B. Savoldo, A. M. Leen, M. Stanojevic, M. Khalil, W. Decker, J. J. Molldrem, H. Liu, A. P. Gee, et al. Functionally active virus-specific T cells that target CMV, adenovirus, and EBV can be expanded from naive T-cell populations in cord blood and will target a range of viral epitopes Blood, August 27, 2009; 114(9): 1958 - 1967. [Abstract] [Full Text] [PDF] B. A. Colleton, X.-L. Huang, N. M. Melhem, Z. Fan, L. Borowski, G. Rappocciolo, and C. R. Rinaldo Primary Human Immunodeficiency Virus Type 1-Specific CD8+ T-Cell Responses Induced by Myeloid Dendritic Cells J. Virol., June 15, 2009; 83(12): 6288 - 6299. [Abstract] [Full Text] [PDF] K. V. Komanduri, L. S. St. John, M. de Lima, J. McMannis, S. Rosinski, I. McNiece, S. G. Bryan, I. Kaur, S. Martin, E. D. Wieder, et al. Delayed immune reconstitution after cord blood transplantation is characterized by impaired thymopoiesis and late memory T-cell skewing Blood, December 15, 2007; 110(13): 4543 - 4551. [Abstract] [Full Text] [PDF] J. N. Barker Umbilical Cord Blood (UCB) Transplantation: An Alternative to the Use of Unrelated Volunteer Donors? Hematology, January 1, 2007; 2007(1): 55 - 61. [Abstract] [Full Text] [PDF]

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