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Blood, 1 September 2006, Vol. 108, No. 5, pp. 1515-1523. Prepublished online as a Blood First Edition Paper on April 27, 2006; DOI 10.1182/blood-2005-11-011874. This Article Full Text (PDF) All Versions of this Article: blood-2005-11-011874v1 108/5/1515    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chang, K.-H. Articles by Papayannopoulou, T. Search for Related Content PubMed PubMed Citation Articles by Chang, K.-H. Articles by Papayannopoulou, T. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 30, 2005 Accepted April 18, 2006 Definitive-like erythroid cells derived from human embryonic stem cells co-express high levels of embryonic and fetal globins with little or no adult globin Kai-Hsin Chang, Angelique M Nelson, Hua Cao, Linlin Wang, Betty Nakamoto, Carol B Ware, and Thalia Papayannopoulou* University of Washington, Department of Medicine, Hematology division, Seattle, WA University of Washington, Department of Comparative Medicine, Seattle, WA University of Washington, Department of Medicine, Medical Genetics division, Seattle, WA * Corresponding author; email: thalp{at}u.washington.edu. Human embryonic stem cells are a promising tool to study events associated with the earliest ontogenetic stages of hematopoiesis. We describe the generation of erythroid cells from hES (H1) by subsequent processing of cells present at early and late stages of EB differentiation. Kinetics of hematopoietic marker emergence suggest that CD45(+) hematopoiesis peaks at late D14EB differentiation stages, although low level CD45(-) erythroid differentiation can be seen before that stage. By morphologic criteria, hES- derived erythroid cells were of definitive type, but these cells both at mRNA and protein levels co-expressed high levels of embryonic ( ) and fetal ( ) globins, with little or no adult globin ( ). This globin expression pattern was not altered by the presence or absence of fetal bovine serum, vascular endothelial growth factor, Flt3-L, or coculture with OP-9, during erythroid differentiation and was not culture time dependent. The co-expression of both embryonic and fetal globins by definitive type erythroid cells does not faithfully mimic either yolk sac embryonic or their fetal liver counterparts. Nevertheless, the high frequency of erythroid cells co- expressing embryonic and fetal globin generated from ES cells can serve as an invaluable tool to further explore molecular mechanisms. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. S. Kaufman Toward clinical therapies using hematopoietic cells derived from human pluripotent stem cells Blood, October 22, 2009; 114(17): 3513 - 3523. [Abstract] [Full Text] [PDF] O. Klimchenko, M. Mori, A. DiStefano, T. Langlois, F. Larbret, Y. Lecluse, O. Feraud, W. Vainchenker, F. Norol, and N. Debili A common bipotent progenitor generates the erythroid and megakaryocyte lineages in embryonic stem cell-derived primitive hematopoiesis Blood, August 20, 2009; 114(8): 1506 - 1517. [Abstract] [Full Text] [PDF] S. L. McKinney-Freeman, O. Naveiras, F. Yates, S. Loewer, M. Philitas, M. Curran, P. J. Park, and G. Q. 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