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Blood, 15 October 2006, Vol. 108, No. 8, pp. 2578-2586. Prepublished online as a Blood First Edition Paper on July 6, 2006; DOI 10.1182/blood-2005-12-010827. This Article Full Text (PDF) All Versions of this Article: blood-2005-12-010827v1 108/8/2578    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Imbert, A.-M. Articles by Chabannon, C. Search for Related Content PubMed PubMed Citation Articles by Imbert, A.-M. Articles by Chabannon, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 1, 2005 Accepted June 15, 2006 CD99 Expressed on human mobilized peripheral blood CD34+ cells is involved in trans-endothelial migration Anne-Marie Imbert*, Ghania Belaaloui, Florence Bardin, Cecile Tonnelle, Marc Lopez, and Christian Chabannon Centre de Therapie Cellulaire et Genique, Institut Paoli-Calmettes, France Equipe d'Hematopoiese Moleculaire et Fonctionnelle, UMR 599 Inserm, France * Corresponding author; email: imbertam{at}marseille.fnclcc.fr. Haematopoietic progenitor cell trafficking is an important phenomenon throughout life. It is thought to occur in sequential steps, similarly to what has been described for mature leukocytes. Molecular actors have been identified for each step of leukocyte migration: recently, CD99 was shown to play a part during trans-endothelial migration. We explored the expression and role of CD99 on human haematopoietic progenitors. We demonstrate that i) CD34+ cells express CD99, albeit with various intensities, ii) subsets of CD34+ cells with high or low levels of CD99 expression produce different numbers of erythroid, NK or dendritic cells in in vitro differentiation assays, iii) the level of CD99 expression is related to the ability to differentiate towards B cells, iv) CD34+ cells that migrate through an endothelial monolayer in response to SDF-1a and SCF display the highest level of CD99 expression, v) binding of a neutralizing antibody to CD99 partially inhibits trans-endothelial migration of CD34+ progenitors in an in vitro assay, vi) binding of a neutralizing antibody to CD99 reduces homing of CD34+ progenitors xeno-transplanted in NOD-SCID mice. We conclude that expression of CD99 on human CD34+ progenitors has functional significance, and that CD99 may be involved in trans-endothelial migration of progenitors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Buxton, C. E. Bacchi, G. Gualco, L. M. Weiss, C. W. Zuppan, E. H. Rowsell, Q. Huang, and J. Wang Frequent Expression of CD99 in Anaplastic Large Cell Lymphoma: A Clinicopathologic and Immunohistochemical Study of 160 Cases Am J Clin Pathol, April 1, 2009; 131(4): 574 - 579. [Abstract] [Full Text] [PDF]

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